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Fabrication of thermoplastic polyurethane tissue engineering scaffold by combining microcellular injection molding and particle leaching

  • Hao-Yang Mi (a1), Xin Jing (a2), Max R. Salick (a3), Lih-Sheng Turng (a4) and Xiang-Fang Peng (a5)...


Microcellular injection molding, a process capable of mass-producing complex plastic parts, and particle leaching methods were combined to fabricate porous thermoplastic polyurethane tissue engineering scaffolds. Water soluble polyvinyl alcohol (PVOH) and sodium chloride (NaCl) were used as porogens to improve the porosity and interconnectivity as well as the hydrophilicity of the scaffolds. It was found in the study that the microcellular injection molding process was effective at producing high pore density and porosity. The addition of PVOH decreased the pore diameter and increased the pore density. Furthermore, scaffolds with NaCl and PVOH porogens showed more interconnected pores. The 3T3 fibroblast cell culture was used to confirm the biocompatibility of the scaffolds. Residual PVOH content after leaching increased the hydrophilicity of the scaffolds and further improved cell adhesion and proliferation. The resulting scaffolds offer an alternative scalable tissue scaffold fabrication method for soft tissue scaffold production.


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1.Wang, X.W., Lin, P., Yao, Q.H., and Chen, C.Y.: Development of small-diameter vascular grafts. World J. Surg. 31, 682 (2007).
2.Jang, D.W., Nguyen, T.H., Sarkar, S.K., and Lee, B.T.: Microwave sintering and in vitro study of defect-free stable porous multilayered HAp-ZrO2 artificial bone scaffold. Sci. Technol. Adv. Mat. 13, (2012).
3.Li, B., Davidson, J.M., and Guelcher, S.A.: The effect of the local delivery of platelet-derived growth factor from reactive two-component polyurethane scaffolds on the healing in rat skin excisional wounds. Biomaterials 30, 3486 (2009).
4.Tian, Z.C., Zhu, Y.L., Qiu, J.J., Guan, H.F., Li, L.Y., Zheng, S.C., Dong, X.H., and Xiao, J.: Synthesis and characterization of UPPE-PLGA-rhBMP2 scaffolds for bone regeneration. J. Huazhong U. Sci.-Med. 32, 563 (2012).
5.Rosado, A.M. and Brewster, L.P.: Regeneration: Letting the scaffold do the work. J. Surg. Res. 180, 49 (2013).
6.Zhang, Y.Z., Venugopal, J., Huang, Z.M., Lim, C.T., and Ramakrishna, S.: Characterization of the surface biocompatibility of the electrospun PCL-collagen nanofibers using fibroblasts. Biomacromolecules 6, 2583 (2005).
7.Yang, S.F., Leong, K.F., Du, Z.H., and Chua, C.K.: The design of scaffolds for use in tissue engineering. Part 1. Traditional factors. Tissue Eng. 7, 679 (2001).
8.Gupta, B., Patra, S., and Ray, A.R.: Preparation of porous polycaprolactone tubular matrix by salt leaching process. J. Appl. Polym. Sci. 126, 1505 (2012).
9.Sherwood, J.K., Riley, S.L., Palazzolo, R., Brown, S.C., Monkhouse, D.C., Coates, M., Griffith, L.G., Landeen, L.K., and Ratcliffe, A.: A three-dimensional osteochondral composite scaffold for articular cartilage repair. Biomaterials 23, 4739 (2002).
10.Shao, X.X., Hutmacher, D.W., Ho, S.T., Goh, J.C.H., and Lee, E.H.: Evaluation of a hybrid scaffold/cell construct in repair of high-load-bearing osteochondral defects in rabbits. Biomaterials 27, 1071 (2006).
11.Odedra, D., Chiu, L., Reis, L., Rask, F., Chiang, K., and Radisic, M.: Cardiac tissue engineering. In Biomaterials for Tissue Engineering Applications: A Review of the Past and Future Trends, Burdick, J.A. and Mauck, R.L. ed.; Springer, New York, 2011; p. 421.
12.Liu, X.H. and Ma, P.X.: Polymeric scaffolds for bone tissue engineering. Ann. Biomed. Eng. 32, 477 (2004).
13.Ghasemi-Mobarakeh, L., Prabhakaran, M.P., Morshed, M., Nasr-Esfahani, M.H., and Ramakrishna, S.: Bio-functionalized PCL nanofibrous scaffolds for nerve tissue engineering. Mat. Sci. Eng. C-Mater. 30, 1129 (2010).
14.Shor, L., Yildirim, E.D., Guceri, S., and Sun, W.: Precision extruding deposition for freeform fabrication of PCL and PCL-HA tissue scaffolds. Biological and Medical Physics. Biomedical Engineering, (Springer-Verlag, New York, 2010); p. 91.
15.Yeo, A., Wong, W.J., and Teoh, S.H.: Surface modification of PCL-TCP scaffolds in rabbit calvaria defects: Evaluation of scaffold degradation profile, biomechanical properties and bone healing patterns. J. Biomed. Mater. Res. A 93, 1358 (2010).
16.Ajami-Henriquez, D., Rodriguez, M., Sabino, M., Castillo, R.V., Muller, A.J., Boschetti-de-Fierro, A., Abetz, C., Abetz, V., and Dubois, P.: Evaluation of cell affinity on poly(L-lactide) and poly(epsilon-caprolactone) blends and on PLLA-b-PCL diblock copolymer surfaces. J. Biomed. Mater. Res. A 87, 405 (2008).
17.Navarro, M., Aparicio, C., Charles-Harris, M., Ginebra, M.P., Engel, E., and Planell, J.A.: Development of a biodegradable composite scaffold for bone tissue engineering: Physicochemical, topographical, mechanical, degradation, and biological properties. Adv. Polym. Sci. 200, 209 (2006).
18.Nieponice, A., Soletti, L., Guan, J.J., Hong, Y., Gharaibeh, B., Maul, T.M., Huard, J., Wagner, W.R., and Vorp, D.A.: In Vivo assessment of a tissue-engineered vascular graft combining a biodegradable elastomeric scaffold and muscle-derived stem cells in a rat model. Tissue Eng. Pt. A 16, 1215 (2010).
19.Danielsson, C., Ruault, S., Simonet, M., Neuenschwander, P., and Frey, P.: Polyesterurethane foam scaffold for smooth muscle cell tissue engineering. Biomaterials 27, 1410 (2006).
20.Hentschel, T. and Munstedt, H.: Thermoplastic polyurethane - the material used for the Erlanger silver catheter. Infection 27, S43 (1999).
21.Maurus, P.B. and Kaeding, C.C.: Bioabsorbable implant material review. Oper. Techn. Sport. Med. 12, 158 (2004).
22.Lamba, N., Woodhouse, K., and Cooper, S.: Polyurethanes in Biomedical Applications (CRC Press, New York, 1998).
23.Huang, C., Chen, R., Ke, Q.F., Morsi, Y., Zhang, K.H., and Mo, X.M.: Electrospun collagen-chitosan-TPU nanofibrous scaffolds for tissue engineered tubular grafts. Colloid Surface B 82, 307 (2011).
24.Dempsey, D.K., Schwartz, C.J., Ward, R.S., Iyer, A.V., Parakka, J.P., and Cosgriff-Hernandez, E.M.: Micropatterning of electrospun polyurethane fibers through control of surface topography. Macromol. Mater. Eng. 295, 990 (2010).
25.Martinez-Perez, C.A., Garcia-Casillas, P.E., Romero, P., Martinez-Villafane, A., Moller, A.D., and Romero-Garcia, J.: Porous biodegradable polyurethane scaffolds prepared by thermally induced phase separation. J. Adv. Mater.-Covina 1, 5 (2006).
26.Sin, D., Miao, X.G., Liu, G., Wei, F., Chadwick, G., Yan, C., and Friis, T.: Polyurethane (PU) scaffolds prepared by solvent casting/particulate leaching (SCPL) combined with centrifugation. Mat. Sci. Eng. C 30, 78 (2010).
27.He, K. and Wang, X.H.: Rapid prototyping of tubular polyurethane and cell/hydrogel constructs. J. Bioact. Compat. Pol. 26, 363 (2011).
28.Ito, S., Matsunaga, K., Tajima, M., and Yoshida, Y.: Generation of microcellular polyurethane with supercritical carbon dioxide. J. Appl. Polym. Sci. 106, 3581 (2007).
29.Leicher, S., Will, J., Haugen, H., and Wintermantel, E.: MuCell (R) technology for injection molding: A processing method for polyether-urethane scaffolds. J. Mater. Sci. 40, 4613 (2005).
30.Gerhardt, L.J., Manke, C.W., and Gulari, E.: Rheology of polydimethylsiloxane swollen with supercritical carbon dioxide. J. Polym. Sci. Pol. Phys. 35, 523 (1997).
31.Wu, L.B., Jing, D.Y., and Ding, J.D.: A “room-temperature” injection molding/particulate leaching approach for fabrication of biodegradable three-dimensional porous scaffolds. Biomaterials 27, 185 (2006).
32.Liu, S.J., Hsueh, C.L., Ueng, S.W.N., Lin, S.S., and Chen, J.K.: Manufacture of solvent-free polylactic-glycolic acid (PLGA) scaffolds for tissue engineering. Asia-Pac. J. Chem. Eng. 4, 154 (2009).
33.Kramschuster, A. and Turng, L.S.: An injection molding process for manufacturing highly porous and interconnected biodegradable polymer matrices for use as tissue engineering scaffolds. J. Biomed. Mater. Res. B. 92, 366 (2010).
34.Naguib, H.E. and Park, C.B.: Strategies for achieving ultra low-density polypropylene foams. Polym. Eng. Sci. 42, 1481 (2002).
35.Gong, P.J. and Ohshima, M.: The effect of interfacial miscibility on the cell morphology of polyethylene terephthalate/bisphenol a polycarbonate blend foams. J. Polym. Sci. Pol. Phys. 50, 1173 (2012).
36.Leung, S.N., Park, C.B., and Li, H.: Numerical simulation of polymeric foaming processes using modified nucleation theory. Plast. Rubber Compos. 35, 93 (2006).
37.Oh, S.H., Kang, S.G., Kim, E.S., Cho, S.H., and Lee, J.H.: Fabrication and characterization of hydrophilic poly(lactic-co-glycolic acid)/poly(vinyl alcohol) blend cell scaffolds by melt-molding particulate-leaching method. Biomaterials 24, 4011 (2003).
38.O'Brien, F.J., Harley, B.A., Yannas, I.V., and Gibson, L.J.: The effect of pore size on cell adhesion in collagen-GAG scaffolds. Biomaterials 26, 433 (2005).


Fabrication of thermoplastic polyurethane tissue engineering scaffold by combining microcellular injection molding and particle leaching

  • Hao-Yang Mi (a1), Xin Jing (a2), Max R. Salick (a3), Lih-Sheng Turng (a4) and Xiang-Fang Peng (a5)...


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