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Effect of Ti6Al4V surface morphology on the osteogenic differentiation of human embryonic stem cells

Published online by Cambridge University Press:  26 October 2017

Leonardo Marasca Antonini ,
Vinícius Kothe ,
Gwendolen C. Reilly ,
Robert Owen ,
Jossano Saldanha Marcuzzo  and
Célia de Fraga Malfatti
Leonardo Marasca Antonini*
Affiliation:
Laboratório de Pesquisa em Corrosão/Programa de Pós-Graduação em Engenharia de Minas, Metalúrgica e de Materiais (LAPEC/PPGE3M), Universidade Federal do Rio Grande do Sul, Porto Alegre, RS 91501-970, Brasil
Vinícius Kothe
Affiliation:
Laboratório de Pesquisa em Corrosão/Programa de Pós-Graduação em Engenharia de Minas, Metalúrgica e de Materiais (LAPEC/PPGE3M), Universidade Federal do Rio Grande do Sul, Porto Alegre, RS 91501-970, Brasil
Gwendolen C. Reilly
Affiliation:
Institute for in silico Medicine (INSIGNEO), University of Sheffield, Sheffield S1 3JD, U.K.
Robert Owen
Affiliation:
Institute for in silico Medicine (INSIGNEO), University of Sheffield, Sheffield S1 3JD, U.K.
Jossano Saldanha Marcuzzo
Affiliation:
Instituto Nacional de Pesquisas Espaciais (INPE), São José dos Campos, SP 12228-970, Brasil
Célia de Fraga Malfatti
Affiliation:
Laboratório de Pesquisa em Corrosão/Programa de Pós-Graduação em Engenharia de Minas, Metalúrgica e de Materiais (LAPEC/PPGE3M), Universidade Federal do Rio Grande do Sul, Porto Alegre, RS 91501-970, Brasil
*
a)Address all correspondence to this author. e-mail: leomantonini@gmail.com
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Abstract

Ti6Al4V alloys usually need to undergo some kind of surface treatment to enable good bone growth and implant integration. In this work, three treatments that modify the titanium alloy surface were studied with the aim of promoting osteogenic differentiation of human-embryonic-stem-cell-derived mesenchymal progenitors (hESC-MPs). The surface treatments used were mechanical polishing and electropolishing for 4 or 12 min in an H2SO4/HF/glycerine solution. The samples were characterised by atomic force microscopy, profilometry, X-ray photoelectron spectroscopy, and wettability. Samples were seeded with hESC-MPs in osteogenic media, and the cell number and alkaline phosphatase activity were measured. The electropolishing surface treatments influenced the nanometric morphology and wettability. However, the electropolished surfaces contributed in the same way as the mechanically polished surface to osteogenic differentiation, indicating that differentiation was strongly influenced by microroughness, which did not differ among the treatments used in the present work.

Keywords

hESC-MPsTi6Al4Velectropolishingcell differentiationsurface treatment
Type
Articles
Information
Journal of Materials Research , Volume 32 , Issue 20 , 27 October 2017 , pp. 3811 - 3821
Copyright
Copyright © Materials Research Society 2017 

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Footnotes

Contributing Editor: Lakshmi Nair

References

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