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Volumetry of human taste buds using laser scanning microscopy

  • T Just (a1), E Srur (a1), O Stachs (a2) and H W Pau (a1)



In vivo laser scanning confocal microscopy is a relatively new, non-invasive method for assessment of oral cavity epithelia. The penetration depth of approximately 200–400 µm allows visualisation of fungiform papillae and their taste buds.

Materials and methods:

This paper describes the technique of in vivo volumetry of human taste buds. Confocal laser scanning microscopy used a diode laser at 670 nm for illumination. Digital laser scanning confocal microscopy equipment consisted of the Heidelberg Retina Tomograph HRTII and the Rostock Cornea Module. Volume scans of fungiform papillae were used for three-dimensional reconstruction of the taste bud.


This technique supplied information on taste bud structure and enabled measurement and calculation of taste bud volume. Volumetric data from a 23-year-old man over a nine-day period showed only a small deviation in values. After three to four weeks, phenomenological changes in taste bud structures were found (i.e. a significant increase in volume, followed by disappearance of the taste bud and appearance of a new taste bud).


The data obtained indicate the potential application of this non-invasive imaging modality: to evaluate variation of taste bud volume in human fungiform papillae with ageing; to study the effects of chorda tympani nerve transection on taste bud volume; and to demonstrate recovery of taste buds in patients with a severed chorda tympani nerve who show recovery of gustatory sensibility after surgery.


Corresponding author

Address for correspondence: Dr Tino Just, Department of Otorhinolaryngology and Head and Neck Surgery, University of Rostock, Doberaner Strasse 137–139, 18057 Rostock, Germany. Fax: +49 (0)381 494 8302 E-mail:


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1Kullaa-Mikkonen, A, Koponen, A, Seilonen, A. Quantitative study of human fungiform papillae and taste buds: variation with aging and in different morphological forms of the tongue. Gerodontics 1987;3:131–5
2Miller, IJ Jr, Reedy, FE Jr. Variations in human taste bud density and taste intensity perception. Physiol Behav 1990;47:1213–19
3Miller, IJ Jr, Reedy, FE Jr. Quantification of fungiform papillae and taste pores in living human subjects. Chem Senses 1990;15:281–94
4Guthoff, RF, Baudouin, C, Stave, J. Atlas of Confocal Laser Scanning In-Vivo Microscopy in Ophthalmology. Berlin, Heidelberg, New York: Springer-Verlag, 2006
5Just, T, Zeisner, C, Stave, J, Pau, HW. Confocal laser-scanning microscopy to analyse the epithelium of the tongue [in German]. Laryngorhinootologie 2004;83:108–12
6Just, T, Stave, J, Pau, HW, Guthoff, R. In vivo observation of papillae of the human tongue using confocal laser scanning microscopy. ORL J Otorhinolaryngol Relat Spec 2005;67:207–12
7Just, T, Pau, HW, Bombor, I, Guthoff, RF, Fietkau, R, Hummel, T. Confocal microscopy of the peripheral gustatory system: comparison between healthy subjects and patients suffering from taste disorders during radiochemotherapy. Laryngoscope 2005;115:2178–82
8Mistretta, CM. Anatomy and neurophysiology of the taste system in aged animals. Ann N Y Acad Sci 1989;561:277–90
9Zeng, Q, Oakley, B. p53 and BAX: putative death factors in taste cell turnover. J Comp Neurol 1999;413:168–80
10Guagliardo, NA, Hill, DL. Fungiform taste bud degeneration in C57BL/6J mice following chorda-lingual nerve transection. J Comp Neurol 2007;504:206–16


Volumetry of human taste buds using laser scanning microscopy

  • T Just (a1), E Srur (a1), O Stachs (a2) and H W Pau (a1)


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