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Protective effect of (-)-epigallocatechin-3-gallate against cisplatin-induced ototoxicity

  • S I Cho (a1), J H Lee (a1), J H Park (a1) and N Y Do (a1)

Abstract

Objective:

Ototoxicity due to cisplatin therapy interferes with treatment and often forces a reduction in the dosage, duration and frequency of the cisplatin therapy. (-)-Epigallocatechin-3-gallate is known to have the highest antioxidant potency among all tea catechins. This study aimed to investigate the effect of (-)-epigallocatechin-3-gallate on cisplatin ototoxicity in an auditory cell line: House Ear Institute-Organ of Corti 1 cells.

Methods:

Cultured House Ear Institute-Organ of Corti 1 cells were exposed to cisplatin with or without pre-treatment with (-)-epigallocatechin-3-gallate. Cell viability was evaluated using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Hoechst 33258 staining was used to identify cells undergoing apoptosis. Western blot analysis was conducted to determine whether (-)-epigallocatechin-3-gallate inhibited cisplatin-induced caspase activation. Intracellular reactive oxygen species production was examined to investigate whether (-)-epigallocatechin-3-gallate was capable of scavenging cisplatin-induced reactive oxygen species accumulation.

Results:

Cell viability significantly increased in cells pre-treated with (-)-epigallocatechin-3-gallate compared with cells exposed to cisplatin alone. Cisplatin increased cleaved caspase-3 on Western blot analysis; however, pre-treatment with (-)-epigallocatechin-3-gallate inhibited the expression of caspase-3. (-)-Epigallocatechin-3-gallate attenuated reactive oxygen species production and apoptosis in House Ear Institute-Organ of Corti 1 cells.

Conclusion:

(-)-Epigallocatechin-3-gallate protected against cisplatin cytotoxicity through anti-apoptotic and anti-oxidative effects. Therefore, (-)-epigallocatechin-3-gallate could play a preventive role in cisplatin-induced ototoxicity.

Copyright

Corresponding author

Address for correspondence: Dr S I Cho, Department of Otolaryngology-Head and Neck Surgery, Chosun University Hospital, 365 Pilmun-daero, Dong-gu, Gwangju 501-717, South Korea Fax: +82-62-225-2702 E-mail: chosi@chosun.ac.kr

References

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1Hartmann, JT, Lipp, HP. Toxicity of platinum compounds. Expert Opin Pharmacother 2003;4:889901
2Hamers, FP, Klis, SF, Gispen, WH, Smoorenburg, GF. Application of a neuroprotective ACTH(4–9) analog to affect cisplatin ototoxicity: an electrocochleographic study in guinea pigs. Eur Arch Otorhinolaryngol 1994;251:23–9
3Dehne, N, Lautemann, J, Petrat, F, Rauen, U, de Groot, H. Cisplatin ototoxicity: involvement of iron and enhanced formation of superoxide anion radicals. Toxicol Appl Pharmacol 2001;174:2734
4Pigeolet, E, Corbisier, P, Houbion, A, Lambert, D, Michiels, C, Raes, M et al. Glutathione peroxidase, superoxide dismutase, and catalase inactivation by peroxides and oxygen derived free radicals. Mech Ageing Dev 1990;51:283–97
5Clerici, WJ, DiMartino, DL, Prasad, MR. Direct effects of reactive oxygen species on cochlear outer hair cell shape in vitro. Hear Res 1995;84:3040
6Rybak, LP, Kelly, T. Ototoxicity: bioprotective mechanisms. Curr Opin Otolaryngol Head Neck Surg 2003;11:328–33
7Wei, H, Meng, Z. Protective effects of epigallocatechin-3-gallate against lead-induced oxidative damage. Hum Exp Toxicol 2011;30:1521–8
8Guo, Q, Zhao, B, Li, M, Shen, S, Xin, W. Studies on protective mechanisms of four components of green tea polyphenols against lipid peroxidation in synaptosomes. Biochim Biophys Acta 1996;1304:210–22
9Mandel, SA, Amit, T, Kalfon, L, Reznichenko, L, Youdim, MB. Targeting multiple neurodegenerative diseases etiologies with multimodel-acting green tea catechins. J Nutr 2008;133:1578–83S
10Landis-Piwowar, KR, Huo, C, Chen, D, Milacic, V, Shi, G, Chan, TH et al. A novel prodrug of the green tea polyphenol (-)-epigallocatechin-3-gallate as a potential anticancer agent. Cancer Res 2007;67:4303–10
11Choi, YT, Jung, CH, Lee, SR, Bae, JH, Baek, WK, Suh, MH et al. The green tea (-)-epigallocatechin gallate attenuates beta-amyloid-induced neurotoxicity in cultured hippocampal neurons. Life Sci 2001;70:603–14
12Nie, G, Cao, Y, Zhao, B. Protective effects of green tea polyphenols and their major component, (-)-epigallocatechin-3-gallate (EGCG), on 6-hydroxydopamine-induced apoptosis in PC12 cells. Redox Rep 2002;7:171–7
13Sheng, R, Gu, ZL, Xie, ML, Zhou, WX, Guo, CY. Epigallocatechin gallate protects H9c2 cardiomyoblasts against hydrogen dioxides-induced apoptosis and telomere attrition. Eur J Pharmacol 2010;641:199206
14Yao, K, Ye, P, Zhang, L, Tan, J, Tang, X, Zhang, Y. Epigallocatechin gallate protects against oxidative stress-induced mitochondria-dependent apoptosis in human lens epithelial cells. Mol Vis 2008;14:217–23
15Park, SJ, Park, SH, Chang, JW, Choi, J, Jung, HH, Im, GJ. Protective effect of klotho protein against cisplatin ototoxicity in an auditory cell line. J Laryngol Otol 2012;126:1003–9
16Chen, FQ, Hill, K, Guan, YJ, Schacht, J, Sha, SH. Activation of apoptotic pathways in the absence of cell death in an inner-ear immortomouse cell line. Hear Res 2012;284:3341
17Wang, D, Lippard, SJ. Cellular processing of platinum anticancer drugs. Nat Rev Drug Discov 2005;4:307–20
18Lee, JE, Nakagawa, T, Kim, TS, Endo, T, Shiga, A, Iguchi, F et al. Role of reactive radicals in degeneration of the auditory system of mice following cisplatin treatment. Acta Otolaryngol 2004;124:1131–5
19Alam, SA, Ikeda, K, Oshima, T, Suzuki, M, Kawase, T, Kikuchi, T et al. Cisplatin-induced apoptotic cell death in Mongolian gerbil cochlea. Hear Res 2000;141:2838
20Watanabe, K, Inai, S, Jinnouchi, K, Baba, S, Yagi, T. Expression of caspase-activated deoxyribonuclease (CAD) and caspase 3 (CPP32) in the cochlea of cisplatin (CDDP)-treated guinea pigs. Auris Nasus Larynx 2003;30:219–25
21Wang, J, Ladrech, S, Pujol, R, Brabet, P, Van De Water, TR, Puel, JL. Caspase inhibitors, but not c-Jun NH 2-terminal kinase inhibitor treatment, prevent cisplatin-induced hearing loss. Cancer Res 2004;64:9217–24
22Rybak, LP, Whitworth, CA. Ototoxicity: therapeutic opportunities. Drug Discov Today 2005;10:1313–21
23Rybak, LP, Whitworth, CA, Mukherjea, D, Ramkumar, V. Mechanisms of cisplatin-induced ototoxicity and prevention. Hear Res 2007;226:157–67
24Ahmad, N, Feyes, DK, Nieminen, AL, Agarwal, R, Mukhtar, H. Green tea constituent epigallocatechin-3-gallate and induction of apoptosis and cell cycle arrest in human carcinoma cells. J Natl Cancer Inst 1997;89:1881–6
25Lin, YL, Lin, JK. (-)-Epigallocatechin-3-gallate blocks the induction of nitric oxide synthase by down-regulating lipopolysaccharide-induced activity of transcription factor nuclear factor-kappaB. Mol Pharmacol 1997;52:465–72
26Mukhtar, H, Ahmad, N. Tea polyphenols: prevention of cancer and optimizing health. Am J Clin Nutr 2000;71:1698–702S
27Kim, CH, Kang, SU, Pyun, J, Lee, MH, Hwang, HS, Lee, H. Epicatechin protects auditory cells against cisplatin-induced death. Apoptosis 2008;13:1184–94
28Chen, D, Milacic, V, Chen, MS, Wan, SB, Lam, WH, Huo, C et al. Tea polyphenols, their biological effects and potential molecular targets. Histol Histopathol 2008;23:487–96
29Koh, SH, Kwon, H, Kim, KS, Kim, J, Kim, MH, Yu, HJ et al. Epigallocatechin gallate prevents oxidative-stress-induced death of mutant Cu/Zn-superoxide dismutase (G93A) motoneuron cells by alteration of cell survival and death signals. Toxicology 2004;202:213–25
30Jin, CF, Shen, SR Sr, Zhao, BL. Different effects of five catechins on 6-hydroxydopamine-induced apoptosis in PC12 cells. J Agric Food Chem 2001;49:6033–8
31Choi, JH, Rhee, IK, Park, KY, Park, KY, Kim, JK, Rhee, SJ. Action of green tea catechin on bone metabolic disorder in chronic cadmium-poisoned rats. Life Sci 2003;73:1479–89
32Young, IS, Woodside, JV. Antioxidants in health and disease. J Clin Pathol 2001;54:176–86
33Nie, G, Jin, C, Cao, Y, Shen, S, Zhao, B. Distinct effects of tea catechins on 6-hydroxydopamine-induced apoptosis in PC12 cells. Arch Biochem Biophys 2002;397:8490
34Gonçalves, MS, Silveira, AF, Teixeira, AR, Hyppolito, MA. Mechanisms of cisplatin ototoxicity: theoretical review. J Laryngol Otol 2013;127:536–41

Keywords

Protective effect of (-)-epigallocatechin-3-gallate against cisplatin-induced ototoxicity

  • S I Cho (a1), J H Lee (a1), J H Park (a1) and N Y Do (a1)

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