Skip to main content Accessibility help

Localisation of basic fibroblast growth factor in cholesteatoma matrix: an immunochemical study

  • M A Hamed (a1), R H Sayed (a1), K Shiogama (a2), M A Eltaher (a1), K Suzuki (a3) and S Nakata (a4)...



To investigate the expression of basic fibroblast growth factor in the matrix of human acquired cholesteatoma compared to the deep meatal skin. This topic does not appear to have been fully investigated before.


An immunochemical study was conducted. Cholesteatoma tissues from adult patients were collected during surgery (n = 19). Control specimens were taken from the deep meatal skin (n = 8) and compared.


A highly significant difference in basic fibroblast growth factor expression was identified between cholesteatoma and skin (mean ± standard error = 58.53 ± 3.6 per cent in cholesteatoma vs 40.6 ± 3.5 per cent in skin; p = 0.005). Both basal and parabasal keratinocytes were stained positive with basic fibroblast growth factor. Additionally, there was specific staining in the basal columnar middle-ear epithelium and mast cell membrane.


Basic fibroblast growth factor plays an active role in proliferative activity of cholesteatoma through its overexpression in basal and parabasal layers of cholesteatoma matrix. Moreover, its expression in the mast cell membrane supports its role in bone resorption activity.


Corresponding author

Author for correspondence: Dr Mahmood A Hamed, Department of Otorhinolaryngology, Faculty of Medicine, Sohag University, East District, Postal code 82524, Sohag, Egypt E-mail:


Hide All

Dr M A Hamed takes responsibility for the integrity of the content of the paper



Hide All
1Hamed, MA, Nakata, S, Sayed, RH, Ueda, H, Badawy, BS, Nishimura, Y et al. Pathogenesis and bone resorption in acquired cholesteatoma: current knowledge and future prospectives. Clin Exp Otorhinolaryngol 2016;9:298308
2Olszewska, E, Wagner, M, Bernal-Sprekelsen, M, Ebmeyer, J, Dazert, S, Hildmann, H et al. Etiopathogenesis of cholesteatoma. Eur Arch Otorhinolaryngol 2004;261:624
3Albino, AP, Kimmelman, CP, Parisier, SC. Cholesteatoma: a molecular and cellular puzzle. Am J Otol 1998;19:719
4Sudhoff, H, Dazert, S, Gonzales, AM, Borkowski, G, Park, SY, Baird, A et al. Angiogenesis and angiogenic growth factors in middle ear cholesteatoma. Am J Otol 2000;21:793–8
5Milewski, C, Fedorowski, A, Stan, AC, Walter, GF. Basic fibroblast growth factor (b-FGF) in the perimatrix of cholesteatoma [in German]. HNO 1998;46:804–8
6Hewitt, SM, Baskin, DG, Frevert, CW, Stahl, WL, Rosa-Molinar, E. Controls for immunohistochemistry: the Histochemical Society's standards of practice for validation of immunohistochemical assays. J Histochem Cytochem 2014;62:693–7
7Fina, M, Baird, A, Ryan, A. Direct application of basic fibroblast growth factor improves tympanic membrane perforation healing. Laryngoscope 1993;103:804–9
8Zhang, Q, Lou, Z. Impact of basic fibroblast growth factor on healing of tympanic membrane perforations due to direct penetrating trauma: a prospective non-blinded/controlled study. Clin Otolaryngol 2012;37:446–51
9Lou, Z, Huang, P, Yang, J, Xiao, J, Chang, J. Direct application of bFGF without edge trimming on human subacute tympanic membrane perforation. Am J Otolaryngol 2016;37:156–61
10Koutnouyan, HA, Baird, A, Ryan, AF. Acidic and basic FGF mRNA expression in the middle ear mucosa during experimental acute and chronic otitis media. Laryngoscope 1994;104:350–8
11Ryan, AF, Baird, A. Growth factors during proliferation of the middle ear mucosa. Acta Otolaryngol 1993;113:6874
12Berger, G, Hawke, M, Ekem, JK. Bone resorption in chronic otitis media. The role of mast cells. Acta Otolaryngol 1985;100:7280
13Albino, AP, Reed, JA, Bogdany, JK, Sassoon, J, Parisier, SC. Increased numbers of mast cells in human middle ear cholesteatomas: implications for treatment. Am J Otol 1998;19:266–72


Localisation of basic fibroblast growth factor in cholesteatoma matrix: an immunochemical study

  • M A Hamed (a1), R H Sayed (a1), K Shiogama (a2), M A Eltaher (a1), K Suzuki (a3) and S Nakata (a4)...


Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed