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Local and systemic effects of low-dose transtympanic methotrexate: in vivo animal study

Published online by Cambridge University Press:  10 December 2012

E Sozen ,
S B Erol ,
O Yildirim ,
B U Coskun ,
T Basak ,
F T Kayhan  and
B Dadas
E Sozen*
Affiliation:
Department of Otorhinolaryngology (Clinic II), Sisli Etfal Training and Research Hospital, Istanbul, Turkey
S B Erol
Affiliation:
Department of Otorhinolaryngology (Clinic II), Sisli Etfal Training and Research Hospital, Istanbul, Turkey
O Yildirim
Affiliation:
Department of Otorhinolaryngology (Clinic II), Sisli Etfal Training and Research Hospital, Istanbul, Turkey
B U Coskun
Affiliation:
Department of Otorhinolaryngology (Clinic II), Sisli Etfal Training and Research Hospital, Istanbul, Turkey
T Basak
Affiliation:
Department of Pathology, Sisli Etfal Training and Research Hospital, Istanbul, Turkey
F T Kayhan
Affiliation:
Department of Otorhinolaryngology, Bakırkoy Dr Sadi Konuk Training and Research Hospital, Istanbul, Turkey
B Dadas
Affiliation:
Department of Otorhinolaryngology (Clinic II), Sisli Etfal Training and Research Hospital, Istanbul, Turkey
*
Address for correspondence: Dr Esra Sozen, Department of Otorhinolaryngology, Clinic II, Sisli Etfal Training and Research Hospital, Istanbul, Turkey Fax: +90 2122962264 E-mail: esrasoz@gmail.com
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Abstract

Objective:

To evaluate the safety of low-dose transtympanic methotrexate in a rat model.

Design:

Experimental animal study.

Setting:

Tertiary training and research hospital.

Methods:

Twenty-four rats were randomly divided into three study groups. Diluted methotrexate solution was administered transtympanically to fill the middle-ear cavity, twice a week in group one and three times a week in group two. Ringer lactate solution was administered transtympanically three times a week in the control group.

Main outcome measures: Local and systemic effects of low-dose transtympanic methotrexate.

Results:

In the methotrexate groups, middle-ear mucosal oedema was present in all animals. Auditory brainstem response thresholds indicated no inner-ear dysfunction in any group. Liver function and serum haemoglobin levels showed no statistically significant difference in any group. However, liver biopsies from groups one and two showed mild portal hyperaemia.

Conclusion:

These findings are encouraging, and support further investigation of the topical application of methotrexate in autoimmune hearing diseases, as an alternative or adjunct to transtympanic steroids.

Keywords

MethotrexateEar, MiddleEar, InnerAutoimmunityDrug Administration Routes
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 127 , Issue 2 , February 2013 , pp. 148 - 152
Copyright
Copyright © JLO (1984) Limited 2012

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