Skip to main content Accessibility help
×
Home

Expression of Ki-67 antigen and proliferative cell nuclear antigen in benign and malignant epithelial lesions of the larynx

  • Nina Zidar (a1), Nina Gale (a1), Andrej Cör (a2) and Vinko Kambič (a3)

Abstract

In an attempt to analyse the proliferative activity in benign and malignant laryngeal epithelial lesions, and to determine the relationship to their histologic grade, we studied the expression of proliferative cell nuclear antigen (PCNA) and Ki-67 antigen on 20 squamous carcinomas, and on 30 biopsies of epithelial hyperplasia categorized according to the Kambiˇ-Lenart classification into simple, abnormal, and atypical hyperplasias. In simple hyperplasia, both antibodies stained the nuclei of the occasional cells in the basal layer. In abnormal hyperplasia (mild dysplasia), positive cells occupied up to a third, and in atypical hyperplasia (moderate and severe dysplasia) they occupied from two-thirds to the entire epithelial thickness. In squamous carcinoma, we have found a statistically significant correlation between its grade and the percentage of Ki-67-(p<0.01) and PCNA-(p<0.00001) positive cells. Our results suggest that the proliferative fraction progressively increases with the degree of epithelial hyperplasia and the grade of carcinoma. We conclude that the patterns of immunoreactivity to PCNA and Ki-67 antigen correspond to the histologic grade of both benign and malignant epithelial lesions of the larynx. This method should be regarded as a useful adjunct to traditional histological techniques allowing more objective grading of benign and malignant epithelial lesions.

Copyright

Corresponding author

Address for correspondence: Nina Zidar, M.D., Institute of Pathology, Medical Faculty, Korytkova 2, 1105 Ljubljana, Slovenia. Fax: +386 61 301 816.

Footnotes

Hide All

A part of the study was presented at the XVth European Congress of Pathology in Copenhagen, September 3–9, 1995.

Footnotes

References

Hide All
Brown, D. C.Gatter, K. C. (1990) Monoclonal antibody Ki-67; its use in histopathology. Histopathology 17: 489503.
Coltrera, M. D., Zarbo, R. J., Sakr, W. A., Gown, A. M. (1992) Markers of dysplasia of the upper aerodigestive tract. American Journal of Pathology 141: 817825.
Crissman, J. D. (1993) Upper aerodigestive tract. In Pathology of Incipient Neoplasia. 2nd Edition. (Henson, D. E., Albores-Saavedra, J., eds.), Saunders, Philadelphia, pp 4463.
Ferlito, A., Friedmann, I. (1993) Squamous cell carcinoma. In Neoplasms of the Larynx. 1st Edition. (Ferlito, A., ed.), Churchill Livingstone, Edinburgh, pp 113133.
Haerslev, T., Jacobsen, G. K. (1995) An immunohistochemical study of p53 with correlations to histopathological parameters, c-erbB-2, proliferating cell nuclear antigen, and prognosis. Human Pathology 26: 295301.
Hall, P. A., Levison, D. A., Woods, A. L., Yu, C. C. W., Kellock, D. B., Watkins, J. A., Barnes, D. M., Gillett, C. E., Camplejohn, R., Dover, R., Waseem, N. H., Lane, D. P. (1990) Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: an index of cell proliferation with evidence of deregulated expression in some neoplasms. Journal of Pathology 162: 285294.
Kambič, V., Lenart, I. (1971) Notre classification des hyperplasies de l'épithelium du larynx au point de vue pronostic. Journal Français d'Oto-Rhino-Laryngologie 20: 11451150.
Kambič, V. (1978) Difficulties in management of vocal cord precancerous lesions. Journal of Laryngology and Otology 92: 305315.
Kambič, V., Gale, N. (1986) Significance of keratosis and dyskeratosis for classifying hyperplastic aberrations of laryngeal mucosa. American Journal of Otolaryngology 7: 323333.
Kambič, V., Gale, N., Ferluga, D. (1992) Laryngeal hyperplastic lesions, follow-up study and application of lectins and anticytokeratins for their evaluation. Pathology, Research and Practice 188: 10671077.
Kambič, V., Gale, N. (1995) Epithelial Hyperplastic Lesions of the Larynx, 1st Edition, Elsevier, Amsterdam, pp 39162.
Kearsley, J. H., Furlong, K. L., Cooke, R. A., Waters, M. J. (1990) An immunohistochemical assessment of cellular proliferation markers in head and neck squamous cell cancers. British Journal of Cancer 61: 821827.
Linden, M. D., Chan, K., Kubus, J., Brown, R. D., Zarbo, R. J. (1993) Ki-67 and proliferating cell nuclear antigen tumour proliferative indices in DNA diploid colorectal adenocarcinomas. American Journal of Clinical Pathology 100: 206212.
Mittal, K. R., Demopoulos, R. I., Goswami, S. (1993) Proliferating cell nuclear antigen (cyclin) expression in normal and abnormal cervical squamous epithelia. American Journal of Surgical Pathology 17: 117122.
Munck-Wikland, E., Edström, S., Jungmark, E., Kuylenstierna, R., Lindholm, J., Auer, G. (1994) Nuclear DNA content, proliferating-cell nuclear antigen (PCNA) and p53 immunostaining in predicting progression of laryngeal cancer in situ lesions. International Journal of Cancer 56: 9599.
Nishioka, H., Hiasa, Y., Hayashi, I., Kitahori, Y., Konishi, N., Sugimura, M. (1993) Immunohistochemical detection of p53 oncoprotein in human oral squamous cell carcinomas and leukoplakias: comparison with proliferating cell nuclear antigen staining and correlation with clinicopathological findings. Oncology 50: 426429.
Sabattini, E., Gerdes, J., Gherlinzoni, F., Poggi, S., Zucchini, L., Melilli, G., Grigioni, F., del Vecchio, M. T., Leoncini, L., Falini, B., Pileri, S. A. (1993) Comparison between the monoclonal antibodies Ki-67 and PC10 in 125 malignant lymphomas. Journal of Pathology 169: 397403.
Scott, R. J., Hall, P. A., Haldane, J. S., Van Noorden, S., Price, Y., Lane, D. P., Wright, N. (1991) A comparison of immunohistochemical markers of cell proliferation with experimentally determined growth fraction. Journal of Pathology 165: 173178.
Shanmugaratnam, K., Sobin, L. H. (1991) Histological Typing of Tumours of the Upper Respiratory Tract and Ear, 2nd Edition, Springer-Verlag, Berlin, pp 2628.
Shin, D. M., Voravud, N., Ro, J. Y., Lee, J. S., Hong, W. K., Hittelman, W. N. (1993) Sequential increases in proliferating cell nuclear antigen expression in head and neck tumorigenesis: A potential biomarker. Journal of the National Cancer Institute 85: 971978.
Shurbaji, M. S., Brooks, S. K., Thurmond, T. S. (1993) Proliferating cell nuclear antigen immunoreactivity in cervical intraepithelial neoplasia and benign cervical epithelium. American Journal of Clinical Pathology 100: 2226.
Thomas, M., Noguchi, M., Kitagawa, H., Kinoshita, K., Miyazaki, I. (1993) Poor prognostic value of proliferating cell nuclear antigen labelling index in breast carcinoma. Journal of Clinical Pathology 46: 525528.

Keywords

Expression of Ki-67 antigen and proliferative cell nuclear antigen in benign and malignant epithelial lesions of the larynx

  • Nina Zidar (a1), Nina Gale (a1), Andrej Cör (a2) and Vinko Kambič (a3)

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed