Skip to main content Accessibility help

Trichrome staining of Gyrodactylus sclerites and soft tissues following fixation in ammonium picrate-glycerin, including an improved rendition of the haptoral bars of G. turnbulli

  • G.R. Richards (a1) and J.C. Chubb (a1)


A simple technique using modified Mallory stain in the transferral of Gyrodactylus specimens from ammonium picrate-glycerin to a permanent mountant is described. Hamuli, their connecting bars and the penis sclerites are well defined by the technique as are muscles and tendons, cell nuclei, tegument and gland cells. As well as being useful in the study of general anatomy, the technique enhances the observation of the taxonomically important ventral and dorsal bars. In order to show this, improved illustrations of the dorsal and ventral bars of G. turnbulli are given along with explicit demonstrations of differences in morphology of the ventral bars of G. bullatarudis and G. rasini - two easily confused species.



Hide All
An, L, Jara, C.A. & Cone, D.K. (1991) Five species of Gyrodactylus Nordmann 1832 (Monogenea) from freshwater fishes of Peru. Canadian journal of Zoology 69,11991202.
Bradbury, P. & Gordon, K.C. (1982) Connective tissue and stains, pp. 122144 in Bancroft, J.D. & Stevens, A. (Eds) Theory and practice of histological techniques. 2nd edn.Edinburgh, London, Melbourne and New York, Churchill Livingstone.
Ergens, R. (1965a) Die morphogenese der chitenoiden Teile des Haptors bei Gyrodactylus decorus Malmberg, 1956 (Monogenoidea) und ihre morphologisch-metrische Variabilität. Zeitschrift für Parasitenkunde 25, 359370.
Ergens, R. (1965b) Die morphogenese der chitenoiden Teile des Haptors bei Gyrodactylus tincae (Malmberg, 1956) Malmberg, 1964 (Monogenoidea) und ihre morpholigisch- metrische Variabilität. Zeitschrift für Parasitenkunde 26, 173184.
Ergens, R. (1969) The suitability of ammonium picrateglycerin in preparing slides of lower Monogenoidea. Folia Parasitologica 16, 320.
Ergens, R. (1983) A survey of the results of studies on Gyrodactylus katherineri Malmberg, 1964 (Gyrodactylidae: Monogenea). Folia Parasitologica 30, 319327.
Harris, P.D. (1986) Species of Gyrodactylus von Nordmann, 1832 (Monogenea Gyrodactylidae) from poeciliid fishes, with a description of G. turnbulli sp. nov. from the guppy, Poecilia reticulata Peters. Journal of Natural History 20, 183191.
Kayton, RJ. (1983) Histochemical and X-ray elemental analysis of the sclerites of Gyrodactylus spp. (Platyhelminthes: Monogenoidea) from the Utah chub, Gila atraria (Girard). Journal of Parasitology 69, 862865.
Kritsky, D.C., Leiby, P.D. & Kayton, R.J. (1978) A rapid stain technique for haptoral bars of Gyrodactylus species (Monogenea). Journal of Parasitology 64, 172174.
Lyons, K.M. (1966) The chemical nature and evolutionary significance of monogenean attachment sclerites. Parasitology 56, 63100.
Malmberg, G. (1956) Om förekomsten av Gyrodactylus på svenska fiskar. Skrifter utigivna av Södra Sveriges Fiskeriforening Êrsskrift 1956, 1976.
Malmberg, G. (1970) The excretory systems and the marginal hooks as a basis for the systematics of Gyrodactylus. Arkiv för Zoologi 23, 1273.
Mo, T.A. & Appleby, C. (1990) A special technique for studying haptoral sclerites of monogeneans. Systematic Parasitology 17, 103108.
Reuter, M. (1987) Immunocytochemical demonstration of serotonin and neuropeptides in the nervous system of Gyrodactylus salaris (Monogenea). Acta zoologica 68, 187193.
Shinn, A.P., Gibson, D.I. & Sommerville, C. (1993) An SEM study of the haptoral sclerites of the genus Gyrodactylus Nordmann, 1832 (Monogenea) following extraction by digestion and sonication techniques. Systematic Parasitology 25, 135144.
Zdárska, Z. (1976) Selective staining of opisthaptor sclerites of some monogeneans. Folia Parasitologica 23, 182.


Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed