Skip to main content Accessibility help
×
Home

Strongyloides ratti: the role of interleukin-5 in protection against tissue migrating larvae and intestinal adult worms

  • K. Watanabe (a1), O. Sasaki (a2), S. Hamano (a3), K. Kishihara (a4), K. Nomoto (a4), I. Tada (a3) and Y. Aoki (a1)...

Abstract

To determine the role of interleukin-5 (IL-5) and eosinophils in protection against Strongyloides ratti, mice treated with anti-IL-5 monoclonal antibody (mAb) were infected with S. ratti larvae. Strongyloides ratti egg numbers in faeces (EPG) in mAb treated mice were higher than those in control mice on days 6 and 7 after inoculation. The numbers of migrating worms in mAb treated mice 36 h after inoculation were higher than those observed in control mice. Intestinal worm numbers in mAb treated mice 5 days after inoculation were higher than those in control mice. These results show that eosinophils effectively protected the host against S. ratti infection by mainly the larval stage in primary infections. The involvement of eosinophils in protection against secondary infection was also examined. Before secondary infection, mice were treated with anti-IL-5 mAb and infected with S. ratti. Patent infections were not observed in either mAb treated or control Ab treated mice. The numbers of migrating worms in the head and lungs of mAb treated mice increased to 60% of that in primary infected mice. Intestinal worms were not found in mAb treated mice or in contcrol mice after oral implantation of adult worms. Eosinophils were therefore mainly involved in protection against tissue migrating worms in secondary infections.

Copyright

Corresponding author

*Fax: +81 95 849 7805 E-mail: kanji@net.nagasaki-u.ac.jp

References

Hide All
Abe, T., Sugaya, H., Ishida, K., Khan, W.I., Tasdemir, I. & Yoshimura, K. (1993) Intestinal protection against Strongyloides ratti and mastocytosis induced by administration of interleukin-3 in mice. Immunology 80, 116121.
Behm, C.A. & Ovington, K.S. (2000) The role of eosinophils in parasitic helminth infections: insights from genetically modified mice. Parasitology Today 16, 202209.
Butterworth, A.E., Sturrock, R.F., Houba, V., Mahmouda, A.F., Sher, A. & Rees, P.H. (1975) Eosinophils as mediators of antibody-dependent damage to schistosomula. Nature 256, 727729.
Coffman, R.L., Seymour, B.W., Hudak, S., Jackson, J. & Rennick, D. (1989) Antibody to interleukin-5 inhibits helminth-induced eosinophilia in mice. Science 245, 308310.
Dawkins, H.J.S., Muir, G.M. & Grove, D.I. (1981) Histopathological appearances in primary and secondary infection with Strongyloides ratti in mice. International Journal for Parasitology 11, 97103.
Herbert, D.R., Lee, J.J., Lee, N.A., Nolan, T.J., Schad, G.A. & Abraham, D. (2000) Role of IL-5 in innate and adaptive immunity to larval Strongyloides stercoralis in mice. Journal of Immunology 165, 45444551.
Hitoshi, Y., Yamaguchi, N., Korenaga, M., Mita, S., Tominaga, A. & Takatsu, K. (1991) In vivo administration of antibody to murine IL-5 receptor inhibits eosinophilia of IL-5 transgenic mice. International Immunology 3, 135139.
Korenaga, M., Hitoshi, Y., Yamaguchi, N., Sato, Y., Takatsu, K. & Tada, I. (1991) The role of interleukin-5 in protective immunity to Strongyloides venezuelensis infection in mice. Immunology 72, 502507.
Maruyama, H., Yabu, Y., Yoshida, A., Nawa, Y. & Ohta, N. (2000) A role of mast cell glycosaminoglycans for the immunological expulsion of the intestinal nematode, Strongyloides venezuelensis. Journal of Immunology 164, 37493754.
Meeusen, E.N.T. & Balic, A. (2000) Do eosinophils have a role in the killing of helminth parasites? Parasitology Today 16, 95101.
Mould, A.W., Matthaei, K.I., Young, I.G. & Foster, P.S. (1997) Relationship between interleukin-5 and eotaxin in regulating blood and tissue eosinophilia in mice. Journal of Clinical Investigation 99, 10641071.
Nawa, Y., Kiyota, M., Korenaga, M. & Kotani, M. (1985) Defective protective capacity of W/Wv mice against Strongyloides ratti infection and its reconstitution with bone marrow cells. Parasite Immunology 7, 429438.
Newlands, G.F.J., Gibson, S., Knox, D.P., Grenics, R., Wakelin, D. & Miller, H.R.P. (1987) Characterization and mast cell origin of a chymotrypsin-like protease isolated from intestines of mice infected with Trichinella spiralis. Immunology 62, 629634.
Onah, D.N., Uchiyama, F., Nagakui, Y., Ono, M., Takai, T. & Nawa, Y. (2000) Mucosal defense against a gastrointestinal nematode: response of mucosal mast cells and mouse mast cell protease 1 during primary Strongyloides venezuelensis infection in FcR gamma-knockout mice. Infection and Immunity 68, 49684971.
Ovington, K.S., McKie, K., Matthaei, K.I., Young, I.G. & Behm, C.A. (1998) Regulation of primary Strongyloides ratti infections in mice: a role for interleukin-5. Immunology 95, 488493.
Shin, S.H., Osada, Y., Chai, J.Y., Matsumoto, N., Takatsu, K. & Kojima, S. (1997) Protective roles of eosinophils in Nippostrongylus braziliensis infection. International Archives of Allergy and Immunology 114, Supplement 1, 4550.
Sugaya, H., Aoki, M., Yoshida, T., Takatsu, K. & Yoshimura, K. (1997) Eosinophilia and intracranial worm recovery in interleukin-5 transgenic and interleukin-5 receptor alpha chain-knockout mice infected with Angiostrongylus cantonensis. Parasitology Research 83, 583590.
Tada, I., Mimori, T. & Nakai, M. (1979) Migration route of Strongyloides ratti in albino rats. Japanese Journal of Parasitology 28, 219227.
Takatsu, K. (1998) Interleukin-5 and B cell differentiation. Cytokine and Growth Factor Reviews 9, 2535.
Watanabe, K., Hamano, S., Noda, K., Koga, M. & Tada, I. (1999) Strongyloides ratti. Additive effect of testosterone implantation and carbon injection on the susceptibility of female mice. Parasitology Research 85, 522526.
Watanabe, K., Noda, K., Hamano, S., Koga, M., Kishihara, K., Nomoto, K. & Tada, I. (2000) The crucial role of granulocytes in the early host defense against Strongyloides ratti infection in mice. Parasitology Research 86, 188193.
Watanabe, K., Hamano, S., Yada, S., Noda, K., Kishihara, K., Nomoto, K. & Tada, I. (2001) The effect of interleukin-4 on the induction of intestinal mast cells and chronological cytokine profiles during intestinal nematode Strongyloides ratti infection. Parasitology Research 87, 149154.

Strongyloides ratti: the role of interleukin-5 in protection against tissue migrating larvae and intestinal adult worms

  • K. Watanabe (a1), O. Sasaki (a2), S. Hamano (a3), K. Kishihara (a4), K. Nomoto (a4), I. Tada (a3) and Y. Aoki (a1)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed