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Specific gravity of Opisthorchis viverrini eggs

Published online by Cambridge University Press:  05 June 2009

T. Harnnoi ,
A. Wijit ,
N. Morakote ,
V. Pipitgool  and
W. Maleewong
T. Harnnoi*
Affiliation:
Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand
A. Wijit
Affiliation:
Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand
N. Morakote
Affiliation:
Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand
V. Pipitgool
Affiliation:
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
W. Maleewong
Affiliation:
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
*
*Fax: 66 53 217144, E-mail: tharnnoi@suandok01.medicine.cmu.ac.th
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Abstract

The specific gravity of the eggs of the liver fluke Opisthorchis viverrini was determined using a sucrose gradient centrifugation and found to range from 1.2713 to 1.3043. The peak egg count was located at the sucrose fraction with a specific gravity of 1.2814. An attempt to float eggs in saturated sodium nitrate solution, sp.gr. 1.4, failed. Examination of human stool specimens for O. viverrini eggs by simple flotation in saturated sodium nitrate solution and the formol-ether sedimentation technique revealed that the flotation technique was not as efficient as the sedimentation technique. It was suggested that the flotation techniques were inappropriate for the detection of O. viverrini eggs in faeces or contaminated soil.

Type
Research Note
Information
Journal of Helminthology , Volume 72 , Issue 4 , December 1998 , pp. 359 - 361
Copyright
Copyright © Cambridge University Press 1998

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