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A simple method for purification of Strongyloides venezuelensis eggs from rat faeces

Published online by Cambridge University Press:  18 November 2020

W.H. Roldán Open the ORCID record for W.H. Roldán [Opens in a new window] ,
F. Martins de Paula  and
R.C.B. Gryschek
W.H. Roldán*
Affiliation:
Laboratório de Imunopatologia da Esquistossomose, Laboratório de Investigação Médica 06 (LIM-06), Hospital das Clínicas da Faculdade de Medicina, USP, São Paulo, Brazil Instituto de Medicina Tropical de São Paulo, USP, São Paulo, Brazil
F. Martins de Paula
Affiliation:
Laboratório de Imunopatologia da Esquistossomose, Laboratório de Investigação Médica 06 (LIM-06), Hospital das Clínicas da Faculdade de Medicina, USP, São Paulo, Brazil Instituto de Medicina Tropical de São Paulo, USP, São Paulo, Brazil
R.C.B. Gryschek
Affiliation:
Laboratório de Imunopatologia da Esquistossomose, Laboratório de Investigação Médica 06 (LIM-06), Hospital das Clínicas da Faculdade de Medicina, USP, São Paulo, Brazil Instituto de Medicina Tropical de São Paulo, USP, São Paulo, Brazil
*
Author for correspondence: W.H. Roldán, E-mail: williamroldang@gmail.com
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Abstract

The aim of this study was to develop a simple method to purify Strongyloides eggs from rat faeces using a sucrose gradient centrifugal-flotation technique. This procedure is simple, rapid and possesses a high efficiency in recovering Strongyloides eggs without faecal detritus in less than one hour, thus eliminating the use of complex apparatus and different chemical substances. The possibility of working with pure and live Strongyloides eggs opens up a wide range of future studies on the biology of this parasite. This study constitutes the first report in the scientific literature on purifying Strongyloides eggs using a sucrose density gradient.

Keywords

Strongyloidesnematodeeggpurificationmethodfaeces
Type
Short Communication
Information
Journal of Helminthology , Volume 94 , 2020 , e210
Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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