Skip to main content Accessibility help
×
Home

Serodiagnostic potential of chemically synthesized glycosphingolipid antigens in an enzyme-linked immunosorbent assay for alveolar echinococcosis

  • K. Yamano (a1), N. Hada (a2), T. Yamamura (a2), T. Takeda (a2), H. Honma (a1) and Y. Sawada (a1)...

Abstract

In the serodiagnosis of alveolar echinococcosis, the detection of specific reactions against not only protein but also carbohydrate antigen is useful and both antigens supplement each other. Though recombinant protein antigens have recently advanced, the preparation of carbohydrate antigen still depends on extraction from crude antigens. In the latter case, it is not conventional to obtain carbohydrate antigen as a single component for examination and research. Therefore, chemically synthesized carbohydrate antigens were prepared for serodiagnosis by the enzyme-linked immunosorbent assay (ELISA). Four antigens with the structure of glycosphingolipids from Echinococcus multilocularis were examined and one antigen, Galβ1-6(Fucα1-3)Galβ1-6Galβ1-ceramide, was found to show significant serodiagnostic potential in differentiating alveolar from cystic echinococcosis.

Copyright

Corresponding author

*Corresponding author: Fax: 81 11 736 9476, Email: yamano@iph.pref.hokkaido.jp

References

Hide All
Felleisen, R. & Gottstein, B. (1994) Comparative analysis of full-length antigen II/3 from Echinococcus multilocularis and E. granulosus. Parasitology 109, 223232.
Frosch, P.M., Frosch, M., Pfister, T., Schaad, V. & Bitter-Suermann, D. (1991) Cloning and characterization of an immunodominant major surface antigen of Echinococcus multilocularis. Molecular and Biochemical Parasitology 48, 121130.
Furuya, K., Sato, H. & Kumagai, M. (1987) Application of Western blotting method for immunoserologic diagnosis of hydatidosis. Journal of Clinical and Experimental Medicine (IGAKU NO AYUMI) 141, 4142 (in Japanese).
Furuya, K., Sasaki, S., Honma, H., Kumagai, M., Sato, N., Takahashi, M. & Uchino, J. (1989) Serologic investigations of human alveolar hydatid disease by Western blotting and indirect histo-immunoperoxidase techniques. Japanese Journal of Parasitology 38, 184193.
Furuya, K., Nishizuka, M., Honma, H., Kumagai, M., Sato, N., Takahashi, M. & Uchino, J. (1990) Prevalence of human alveolar echinococcosis in Hokkaido as evaluated by Western blotting. Japanese Journal of Medical Science and Biology 43, 4349.
Furuya, K., Kawanaka, M., Yamano, K., Sato, N. & Honma, H. (2004) Laboratory evaluation of commercial immunoblot assay kit for serodiagnosis of Echinococcus infections using sera from patients with alveolar hydatidosis in Hokkaido. Journal of the Japanese Association for Infectious Diseases 78, 320326.
Hada, N., Hayashi, E. & Takeda, T. (1999) Synthetic studies on glycosphingolipids from the parasite Echinococcus multilocularis. Carbohydrate Research 316, 5870.
Helbig, M., Frosch, P., Kern, P. & Frosch, M. (1993) Serological differentiation between cystic and alveolar echinococcosis by use of recombinant larval antigens. Journal of Clinical Microbiology 31, 32113215.
Hubert, K., Cordero, E., Frosch, M. & Solomon, F. (1999) Activities of the EM10 protein from Echinococcus multilocularis in cultured mammalian cells demonstrate functional relationships to ERM family members. Cell Motility and the Cytoskeleton 42, 178188.
Hülsmeier, A.J., Gehrig, P.M., Geyer, R., Sack, R., Gottstein, B., Deplazes, P. & Köler, P. (2002) Major Echinococcus multilocularis antigen is a mucin-type glycoprotein. Journal of Biological Chemistry 277, 57425748.
Ito, A., Xiao, N., Liance, M., Sato, M.O., Sako, Y., Mamuti, W., Ishikawa, Y., Nakao, M., Yamasaki, H., Nakaya, K., Bardonnet, K., Bresson-Hadni, S. & Vuitton, D.A. (2002) Evaluation of an enzyme-linked immunosorbent assay (ELISA) with affinity-purified Em18 and an ELISA with recombinant Em18 for differential diagnosis of alveolar echinococcosis: results of a blind test. Journal of Clinical Microbiology 40, 41614165.
Kannagi, R., Nudelman, E., Levery, S.B. & Hakomori, S. (1982) A series of human erythrocyte glycosphingolipids reacting to the monoclonal antibody directed to developmentally-regulated antigen, SSEA-1. Journal of Biological Chemistry 257, 1486514874.
Kimura, H., Furuya, K., Kawase, S., Sato, C., Yamano, K., Takahashi, K., Uraguchi, K., Ito, T., Yagi, K. & Sato, N. (1999) Recent epidemiologic trends in alveolar echinococcosis prevalence in humans and animals in Hokkaido. Journal of the Japanese Association for Infectious Diseases 52, 117120.
Kouguchi, H., Suzuki, T., Yamano, K., Honma, H. & Sawada, Y. (2005) Characterization of various recombinant antigens from Echinococcus multilocularis for use in the immunodiagnosis. Protein Journal 24, 5764.
Marcus, D.M., Kundu, S.K. & Suzuki, A. (1981) The P blood group system: recent progress in immunochemistry and genetics. Seminars in Hematology 18, 6371.
Nagano, M., Sato, C. & Furuya, K. (1995) Human alveolar echinococcosis seroprevalence assessed by Western blotting in Hokkaido. Japanese Journal of Medical Science and Biology 48, 157161.
Persat, F., Vincent, C., Mojon, M. & Petavy, A.F. (1991) Detection of antibodies against glycolipids of Echinococcus multilocularis metacestodes in sera of patients with alveolar hydatid disease. Parasite Immunology 13, 379389.
Persat, F., Bouhours, J.F., Mojon, M. & Petavy, A.F. (1992) Glycosphingolipids with Galβ1-6Gal sequences in metacestodes of the parasite Echinococcus multilocularis. Journal of Biological Chemistry 267, 87648769.
Sato, C. & Furuya, K. (1994) Isolation and characterization of a diagnostic polysaccharide antigen from larval Echinococcus multilocularis. Japanese Journal of Medical Science and Biology 47, 6571.
Sato, H., Mitamura, H., Arai, J. & Kumagai, M. (1983a) Serological diagnosis of human hydatid diseases by enzyme-linked immunosorbent assay (Part 1) enzyme-linked immunosorbent assay by multilocular Echinococcus antigen. Report of the Hokkaido Institute of Public Health 33, 815 (in Japanese).
Sato, H., Mitamura, H., Arai, J. & Kumagai, M. (1983b) Serological diagnosis of human hydatid diseases by enzyme-linked immunosorbent assay (Part 2) application and evaluation on the health examination. Report of the Hokkaido Institute of Public Health 33, 1620 (in Japanese).
Vogel, M., Gottstein, B., Muller, N. & Seeback, T. (1988) Production of a recombinant antigen of Echinococcus multilocularis with high immunodiagnostic sensitivity and specificity. Molecular and Biochemical Parasitology 31, 117125.
Watkins, W.M. (1980) Biochemistry and genetics of the ABO, Lewis and P blood group systems. Advances in Human Genetics 10, 1136.
Yamamura, T., Hada, N., Kaburaki, A., Yamano, K. & Takeda, T. (2004) Synthetic studies on glycosphingolipids from Protostomia phyla: total syntheses of glycosphingolipids from the parasite, Echinococcus multilocularis. Carbohydrate Research 339, 24792759.
Yamano, K., Yagi, K., Furuya, K., Sawada, Y. & Honma, H. (2005) Active alveolar hydatidosis with sero-negativity for antibody to the 18 kDa antigen. Journal of the Japanese Association for Infectious Diseases 58, 122124.

Related content

Powered by UNSILO

Serodiagnostic potential of chemically synthesized glycosphingolipid antigens in an enzyme-linked immunosorbent assay for alveolar echinococcosis

  • K. Yamano (a1), N. Hada (a2), T. Yamamura (a2), T. Takeda (a2), H. Honma (a1) and Y. Sawada (a1)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed.