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Sequence variation in two mitochondrial DNA regions and internal transcribed spacer among isolates of the nematode Oesophagostomum asperum originating from goats in Hunan Province, China

  • F. Li (a1), T. Hu (a1), N.C. Duan (a1), W.Y. Li (a2), Q. Teng (a2), H. Li (a2), W. Liu (a1), Y. Liu (a1) and T.Y. Cheng (a1)...


The present study examined sequence variability in two mitochondrial DNA (mtDNA) regions, namely cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit 1 (nad1), and internal transcribed spacer (ITS) of nuclear ribosomal DNA (rDNA) among Oesophagostomum asperum isolates from goats in Hunan Province, China. A portion of the cox1 (pcox1), nad1 (pnad1) genes and the ITS (ITS1+5.8S rDNA+ITS2) rDNA were amplified by polymerase chain reaction (PCR) separately from adult O. asperum individuals and the representative amplicons were subjected to sequencing from both directions. The lengths of pcox1, pnad1 and ITS rDNA were 366 bp, 681 bp and 785 bp, respectively. The A+T contents of gene sequences were 71.5–72% for pcox1, 73.7–74.2% for pnad1 and 58–58.8% for ITS rDNA. Intra-specific sequence variations within O. asperum were 0–1.6% for pcox1, 0–1.9% for pnad1 and 0–1.7% for ITS rDNA, while inter-specific sequence differences among members of the genus Oesophagostomum were significantly higher, being 11.1–12.5%, 13.3–17.7% and 8.5–18.6% for pcox1, pnad1 and ITS rDNA, respectively. Phylogenetic analyses using combined sequences of pcox1 and pnad1, with three different computational algorithms (Bayesian inference, maximum likelihood and maximum parsimony), revealed distinct groups with high statistical support. These findings demonstrated the existence of intra-specific variation in mtDNA and rDNA sequences among O. asperum isolates from goats in Hunan Province, China, and have implications for studying molecular epidemiology and population genetics of O. asperum.


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Blouin, M.S. (2002) Molecular prospecting for cryptic species of nematodes: mitochondrial DNA versus internal transcribed spacer. International Journal for Parasitology 32, 527531.
Bowles, J., Blair, D. & McManus, D.P. (1992) Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Molecular and Biochemical Parasitology 54, 165173.
Bowman, D.D. (2009) Georgis' parasitology for veterinarians. 9th edn. pp. 176177. Philadelphia, Saunders.
Chen, J., Yang, Z.M., Wang, J.P., Wang, K.G., Tian, X.G. & Wen, M. (2010) Parasitic infection status of Xiaoxiang goat in Leishan count. Guizhou Agricultural Science 38, 180181(in Chinese).
Chilton, N.B., Gasser, R.B. & Beveridge, I. (1995) Differences in a ribosomal DNA sequence of morphologically indistinguishable species within the Hypodontus macropi complex (Nematoda: Strongyloidea). International Journal for Parasitology 25, 647651.
Dai, R.S., Liu, G.H., Song, H.Q., Lin, R.Q., Yuan, Z.G., Li, M.W., Huang, S.Y., Liu, W. & Zhu, X.Q. (2012) Sequence variability in two mitochondrial DNA regions and internal transcribed spacer among three cestodes infecting animals and humans from China. Journal of Helminthology 86, 245251.
de Gruijter, J.M., Blotkamp, J., Gasser, R.B., Amponsah, S. & Polderman, A.M. (2006) Morphological variability within Oesophagostomum bifurcum among different primate species from Ghana. Journal of Helminthology 80, 357361.
Dobson, R.J., Barnes, E.H., Birclijin, S.D. & Gill, J.H. (1992) The survival of Ostertagia circumcincta and Trichostrongylus colubriformis in faecal culture as a source of bias in apportioning egg counts to worm species. International Journal for Parasitology 22, 10051008.
Gasser, R.B., de Gruijter, J.M. & Polderman, A.M. (2006) Insights into the epidemiology and genetic make-up of Oesophagostomum bifurcum from human and non-human primates using molecular tools. Parasitology 132, 453460.
Guindon, S. & Gascuel, O. (2003) A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Systematic Biology 52, 696704.
Jacquiet, P., Colas, F., Cabaret, J., Dia, M.L., Cheikh, D. & Thiam, A. (1995) Dry areas: an example of seasonal evolution of helminth infection of sheep and goats in southern Mauritania. Veterinary Parasitology 56, 137148.
Jia, Y.Q., Zhao, G.H., Hu, B., Cheng, W.Y., Du, S.Z., Bian, Q.Q., Ma, X.T. & Yu, S.K. (2014) Genetic variability in the three mitochondrial genes among Oesophagostomum asperum isolates from different regions in Shaanxi and Hunan Provinces, China. Mitochondrial DNA 25, 298302.
Krief, S., Vermeulen, B., Lafosse, S., Kasenene, J.M., Nieguitsila, A., Berthelemy, M., L'Hostis, M., Bain, O. & Guillot, J. (2010) Nodular worm infection in wild chimpanzees in Western Uganda: a risk for human health? PLoS Neglected Tropical Diseases 4, e630.
Legesse, M. & Erko, B. (2004) Zoonotic intestinal parasites in Papio anubis (baboon) and Cercopithecus aethiops (vervet) from four localities in Ethiopia. Acta Tropica 90, 231236.
Li, J.Y., Liu, G.H., Wang, Y., Song, H.Q., Lin, R.Q., Zou, F.C., Liu, W., Xu, M.J. & Zhu, X.Q. (2013) Sequence variation in three mitochondrial DNA genes among isolates of Ascaridia galli originating from Guangdong, Hunan and Yunnan provinces, China. Journal of Helminthology 87, 371375.
Li, M.W., Lin, R.Q., Song, H.Q., Sani, R.A., Wu, X.Y. & Zhu, X.Q. (2008) Electrophoretic analysis of sequence variability in three mitochondrial DNA regions for ascaridoid parasites of human and animal health significance. Electrophoresis 29, 29122917.
Librado, P. & Rozas, J. (2009) DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25, 14511452.
Lin, R.Q., Liu, G.H., Song, H.Q., Zhang, Y., Li, M.W., Zou, F.C., Yuan, Z.G., Weng, Y.B. & Zhu, X.Q. (2012) Sequence variability in three mitochondrial genes between the two pig nodule worms Oesophagostomum dentatum and O. quadrispinulatum. Mitochondrial DNA 23, 182186.
Lin, R.Q., Liu, G.H., D'Amelio, S., Zhang, Y., Song, H.Q., Weng, Y.B., Zou, F.C. & Zhu, X.Q. (2013) Sequence variation in four mitochondrial genes among sibling species within Contracaecum rudolphii sensu lato. Molecular and Cellular Probes 27, 145148.
Liu, G.H., Zhou, W., Nisbet, A.J., Xu, M.J., Zhou, D.H., Zhao, G.H., Wang, S.K., Song, H.Q., Lin, R.Q. & Zhu, X.Q. (2014) Characterization of Trichuris trichiura from humans and T. suis from pigs in China using internal transcribed spacers of nuclear ribosomal DNA. Journal of Helminthology 88, 6468.
Makouloutou, P., Setsuda, A., Yokoyama, M., Tsuji, T., Saita, E., Torii, H., Kaneshiro, Y., Sasaki, M., Maeda, K., Une, Y., Hasegawa, H. & Sato, H. (2013) Genetic variation of Gongylonema pulchrum from wild animals and cattle in Japan based on ribosomal RNA and mitochondrial cytochrome c oxidase subunit I genes. Journal of Helminthology 87, 326335.
McManus, D.P. & Bowles, J. (1996) Molecular genetics approaches to parasite identification: their value in diagnostic parasitology and systematics. International Journal for Parasitology 26, 687704.
Meng, J.B., Gao, X.Y., Wen, Z.Y., Du, X.P., Wang, N., Wang, J., Meng, P., Su, C.Y., Hu, B., Jin, H.L. & Yu, S.K. (2012) Primary investigation on parasitic helminth species and infection species and infection status in Shenmu country. Progress Veterinary Medicine 33, 135136(in Chinese).
Page, R.D. (1996) TREEVIEW: an application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences 12, 357358.
Pekmezci, G.Z., Onuk, E.E., Bolukbas, C.S., Yardimci, B., Gurler, A.T., Acici, M. & Umur, S. (2014) Molecular identification of Anisakis species (Nematoda: Anisakidae) from marine fishes collected in Turkish waters. Veterinary Parasitology 201, 8294.
Pfukenyi, D.M. & Mukaratirwa, S. (2013) A review of the epidemiology and control of gastrointestinal nematode infections in cattle in Zimbabwe. Onderstepoort Journal of Veterinary Research 80, 612.
Posada, D. (2008) jModelTest phylogenetic model averaging. Molecular Biology and Evolution 25, 12531256.
Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19, 15721574.
Romstad, A., Gasser, R.B., Monti, J.R., Polderman, A.M., Nansen, P., Pit, D.S. & Chilton, N.B. (1997) Differentiation of Oesophagostomum bifurcum from Necator americanus by PCR using genetic markers in spacer ribosomal DNA. Molecular and Cellular Probes 11, 169176.
Sissay, M.M., Uggla, A. & Waller, P.J. (2007) Prevalence and seasonal incidence of nematode parasites and fluke infections of sheep and goats in eastern Ethiopia. Tropical Animal Health and Production 39, 521531.
Swofford, D.L. (2012) Paup*: Phylogenetic analysis using parsimony, version 4.0b10. Sunderland, Massachusetts, Sinauer Associates.
Talavera, G. & Castresana, J. (2007) Improvement of phylogenies after removing divergent and ambiguously aligned blocks from protein sequence alignments. Systematic Biology 56, 564577.
Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. & Higgins, D.G. (1997) The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 24, 48764882.
Wang, Y., Liu, G.H., Li, J.Y., Xu, M.J., Ye, Y.G., Zhou, D.H., Song, H.Q., Lin, R.Q. & Zhu, X.Q. (2013) Genetic variability among Trichuris ovis isolates from different hosts in Guangdong Province, China revealed by sequences of three mitochondrial genes. Mitochondrial DNA 24, 5054.
Zhao, G.H., Mo, X.H., Zou, F.C., Weng, Y.B., Lin, R.Q., Xia, C.M. & Zhu, X.Q. (2009) Genetic variability among Schistosoma japonicum isolates from different endemic regions in China revealed by sequences of three mitochondrial DNA genes. Veterinary Parasitology 162, 6774.
Zhao, G.H., Hu, B., Song, J.K., Jia, Y.Q., Li, H.M., Wang, C.R., Lin, Q., Xu, Q.X., Yu, S.K. & Deng, Y. (2014) Characterization of Oesophagostomum asperum and O. columbianum by internal transcribed spacers of nuclear ribosomal DNA. Journal of Helminthology 88, 7481.
Zhao, L., Liu, G.H., Zhao, G.H., Cai, J.Z., Zhu, X.Q. & Qian, A.D. (2013) Genetic differences between Chabertia ovina and C. erschowi revealed by sequence analysis of four mitochondrial genes. Mitochondrial DNA 24, 263266.
Zhu, X.Q., Chilton, N.B., Jacobs, D.E., Boes, J. & Gasser, R.B. (1999) Characterisation of Ascaris from human and pig hosts by nuclear ribosomal DNA sequences. International Journal for Parasitology 29, 469478.

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Sequence variation in two mitochondrial DNA regions and internal transcribed spacer among isolates of the nematode Oesophagostomum asperum originating from goats in Hunan Province, China

  • F. Li (a1), T. Hu (a1), N.C. Duan (a1), W.Y. Li (a2), Q. Teng (a2), H. Li (a2), W. Liu (a1), Y. Liu (a1) and T.Y. Cheng (a1)...


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