Hostname: page-component-8448b6f56d-wq2xx Total loading time: 0 Render date: 2024-04-24T06:25:06.068Z Has data issue: false hasContentIssue false
  • English
  • Français

Schistosoma mansoni: an in vivo study of drug-induced autophagy in the gastrodermis

Published online by Cambridge University Press:  18 November 2009

Johanna Clarkson  and
D. A. Erasmus
Johanna Clarkson
Affiliation:
Department of Zoology, University college, Cardiff, Wales
Get access Rights & Permissions [Opens in a new window]

Abstract

The effects of Astiban, Lucanthone, Hycanthone and Niridazole on autophagic activities in the gastrodermis of Schistosoma mansoni were determined in vivo, using different dosage levels and dosage times. With Astiban, high levels of autophagy were observed in the gastrodermis 2 hours after an injection of the drug into the mouse, and this response had declined by 20 hours, marking a recovery by the parasite from the drug. Hycanthone and Lucanthone produced an autophagic response several days after the onset of treatment, and no recovery was observed in the morphology of the gastrodermis after the drug was discontinued. The effects of Niridazole on the gastrodermis were to produce the most dramatic ultrastructural changes after high doses and over several days of treatment. With all the drugs examined, gastrodermal autophagy was characterized by the formation of vacuoles containing cell components, lipid droplets and sometimes hydrolytic enzyme reaction product. The autophagic vacuoles appeared to be formed by the sequestration of cytoplasmic material by the basal membrane infoldings, and the transfer of enzymes into the vacuole from within the limiting membrane. The residues from intracellular digestion appeared to be emptied into the caecal lumen.

Type
Research Note
Information
Journal of Helminthology , Volume 58 , Issue 1 , March 1984 , pp. 59 - 68
Copyright
Copyright © Cambridge University Press 1984

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Archer, S. & Yarinsky, A. (1972) Recent developments in the chemotherapy of schistosomiasis. Progress in Drug Research, 16, 12–62.Google ScholarPubMed
Bogitsh, B.J. (1975) Cytochemistry of gastrodermal autophagy following starvation in Schistosoma mansoni. Journal of Parasitology, 61, 237–248.CrossRefGoogle ScholarPubMed
Bueding, E. (1950) Carbohydrate metabolism of Schistosoma mansoni. Journal of General Physiology, 33, 475–495.CrossRefGoogle ScholarPubMed
Bueding, E. & Fisher, J. (1969) Biochemical effects of schistosomicides. Annals of the New York Academy of Sciences, 160, 536–543.CrossRefGoogle ScholarPubMed
Buttle, G.A.H. & Khayyal, M.T. (1962) Rapid hepatic shift of worms in mice infected with Schistosoma mansoni after two single injections of tartar emetic. Nature, 194, 780–781.Google Scholar
Clarkson, J. (1980) Schistosoma mansoni: studies on the structure and functioning of the gut. Ph.D. Thesis, University of Wales.Google Scholar
Coles, G.C. (1973) The metabolism of schistosomes: a review. International Journal of Biochemistry, 4, 319–337.CrossRefGoogle Scholar
Coles, G.C. & Chappell, C.H. (1979) Schistosoma mansoni: effects of antimony in immature and adult worms. Experimental Parasitology, 47, 49–53.CrossRefGoogle ScholarPubMed
De duve, C. (1964) From cytases to lysosomes. Federation Proceedings, 23, 1045–1049.Google Scholar
Duvall, R.H. & Dewitt, W.B. (1967) An improved perfusion technique for recovering adult schistosomes from laboratory animals. American Journal of Tropical Medicine and Hygiene, 16, 483–486.CrossRefGoogle ScholarPubMed
Erasmus, D.A. (1974) The application of X-ray analysis in the transmission electron microscope to a study of drug distribution in the parasite Schistosoma mansoni (Platyhelminthes). Journal of Microscopy, 102, 59–69.CrossRefGoogle ScholarPubMed
Erasmus, D.A. (1975) Schistosoma mansoni: development of the vitelline cell, its role in drug sequestration and changes induced by Astiban. Experimental Parasitology, 38, 240–256.CrossRefGoogle ScholarPubMed
Erasmus, D.A. & Popiel, I. (1980) Schistosoma mansoni: drug induced changes in the cell population of the vitelline gland. Experimental Parasitology, 50, 171–187.CrossRefGoogle ScholarPubMed
Ericsson, J.L.E. (1969) Mechanics of cellular autophagy. In: Lysosomes in Biology and Pathology (Dingle, J.T. and Fell, H.B., Editors), Vol. 2, pp. 345–394. North-Holland Publ. Co.: Amsterdam.Google Scholar
Friedheim, E.A.H. (1973) Chemotherapy of schistosomes. In: Chemotherapy of Helminthiasis (Cavier, R. and Hawking, F., Editors). Vol. 1, pp. 29–144. Pergamon Press: Oxford.Google Scholar
Hernandez, P., Dennis, E.W. & Farah, A. (1971) Metabolism of the schistosomidical agent Hycanthone by rats and monkeys. Bulletin of the World Health Organization, 45, 27–34.Google Scholar
Katz, N. (1977) Chemotherapy of schistosomiasis mansoni. Advances in Pharmacology and Chemotherapy, 14, 1–70.CrossRefGoogle ScholarPubMed
Katz, N. & Pellegrino, J. (1974) Experimental chemotherapy of schistosomiasis mansoni. Advances in Parasitology, 12, 369–390.CrossRefGoogle ScholarPubMed
Khayyal, M.T., Girgis, NI. & Mcconnell, E. (1968) The possible inactivation of antimony by schistosomes. Annals of Tropical Medicine and Parasitology, 62, 74–80.CrossRefGoogle ScholarPubMed
Magzoub, M. (1971) Electron microscopy of normal and treated Schistosoma mansoni. Sudan Medical Journal, 9, 178–182.Google Scholar
Molokhia, M.M. & Smith, H. (1969) Tissue distribution of trivalent antimony in mice infected with Schistosoma mansoni. Bulletin of the World Health Organization. 40, 123–128.Google ScholarPubMed
Novikoff, A.B. & Goldfischer, S. (1961) Nucleoside diphosphatase activity in the Golgi apparatus and its usefulness for cytological studies. Journal of Histochemistry and Cytochemistry, 47, 802–810.Google ScholarPubMed
Popiel, I. & Erasmus, D.A. (1981) Schistosoma mansoni: Niridazole-induced damage to the vitelline gland. Experimental Parasitology, 52, 35–48.CrossRefGoogle Scholar
Ryder, T.A. & Bowen, I.D. (1975) A method for the fine structural localisation of acid phosphatase activity using p-nitrophenyl phosphate as substrate. Journal of Histochemistry and Cytochemistry, 23, 235–237.Google Scholar
Senft, A.W. (1969) Considerations of schistosomc physiology in the search for anti-bilharziasis drugs. Annals of the New York Academy of Sciences, 160, 571–592.CrossRefGoogle ScholarPubMed
Senft, A.W., Senft, D.G., Hillman, G.R., Polk, D. & Kryger, S. (1976) Influence of Hycanthone on morphology and serotonin uptake of Schistosoma mansoni. American Journal of Tropical Medicine and Hygiene, 25, 832–840.CrossRefGoogle Scholar
Smithers, S.R. & Terry, R.J. (1965) The infection of laboratory hosts with cercariae of Schistosoma mansoni and the recovery of adult worms. Parasitology, 55, 695–700.CrossRefGoogle ScholarPubMed
Standen, O.D. (1953) Experimental schistosomiasis III Chemotherapy and mode of drug action. Annals of Tropical Medicine and Parasitology, 47, 26–43.CrossRefGoogle ScholarPubMed
Striebel, H.P. (1969) The effects of Niridazole in experimental schistosomiasis. Annals of the New York Academy of Sciences, 165, 491–518.CrossRefGoogle Scholar