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Scanning and transmission electron microscopy of the female reproductive system of Schistosoma margrebowiei Le Roux, 1933

Published online by Cambridge University Press:  05 June 2009

A. H. H. Awad  and
A. J. Probert
A. H. H. Awad
Affiliation:
School of Biological Sciences, University of Wales, Bangor, Gwynedd LL57 2UW
A. J. Probert
Affiliation:
School of Biological Sciences, University of Wales, Bangor, Gwynedd LL57 2UW
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Abstract

Transmission electron microscopy shows that the uterus of female Schistosoma margrebowiei possesses the same ultrastructure as that of the tegument but lacks spines and sense organs. It does not possess secretory cells and opens at the gonopore which by scanning electron microscopy was seen to be composed of numerous leaf-like protrusions. The morphology of the ovary is comparable with that of other Digenea. Immature and mature ova possess cortically arranged granules and occur within the posterior zone of the ovary. Cilia and lamellae line the luminal surface of the oviduct and ootype, the lamellae running unidirectionally along the duct. Only a single type of secretory cell is seen within Mehlis' gland and this produces dense bodies which are associated with Goldi bodies. Narrow cytoplasmic channels supported by microtubules deliver these secretory bodies to the ootype. The vitelline duct is lined with cilia and lamellae and the vitelline gland contains four types of cells, S1, S2, S3 and S4. Calcareous corpuscles are found within mature S4 cells.

Keywords

Schistosoma margrebowieifemale reproductive systemscanning electron microscopytransmission electron microscopyultrastructure
Type
Research Article
Information
Journal of Helminthology , Volume 64 , Issue 3 , September 1990 , pp. 181 - 192
Copyright
Copyright © Cambridge University Press 1990

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References

REFERENCES

Anderson, E. (1968) Oocyte differentiation in the sea urchin Arbacia punlulata with particular reference to the origin of the cortical granules and their participation in the cortical reactions. Journal of Cell Biology, 37, 514–539.Google Scholar
Awad, A. H. H. & Probert, A. J. (1989) Scanning and transmission electron microscopy of the male reproductive system of Schistosoma margrebowiei Le Roux 1933. Journal of Helminthology, 63, 197–205.CrossRefGoogle Scholar
Bjorkman, N. & Thorsell, W. (1964) On the ultrastructure of the ovary of the liver fluke Fasciola hepatica. Zeitschrift für Zellforschung und Mikroskopische Anatomie, 63, 538–549.CrossRefGoogle Scholar
Bogitsh, B. T. (1970) Observations on the cytochemistry of the Mehlis' gland cells of Haematoloechus medioplexus. Journal of Parasitology, 56, 1084–1094.CrossRefGoogle Scholar
Burton, P. R. (1960) Gametogenesis and fertilization in the frog lung fluke Haematoloechus medioplexus Stafford (Trematoda: Plagiorchiidae). Journal of Morphology, 107, 93–122.Google Scholar
Burton, P. R. (1967) Fine structure of the reproductive system of a frog lung fluke I. Mehlis' gland and associated ducts. Journal of Parasitology, 53, 540–555.Google Scholar
Clegg, J. A. (1965) Secretion of lipoprotein by Mehlis' gland in Fasciola hepatica. Annals of the New York Academy of Science, 118, 969–986.Google Scholar
Coil, W. H. (1963) Observations on the histochemistry of Syncoelium spathulatum n.sp. Proceedings of the Helminthological Society of Washington, 30, 60–65.Google Scholar
Coil, W. H. (1965) Observations on egg-shell formation in Hydrophitrema gigantica Sanders 1960 (Hemiuridae: Digenea). Zeitschrift für Parasitenkunde, 25, 510–517.Google Scholar
Coil, W. H. (1966) Egg shell formation in the notocotylid trematode Ogmocotyle indica (Bhalerao 1942) Ruiz 1946. Zeitschrift für Parasitenkunde, 27, 205–209.CrossRefGoogle ScholarPubMed
Coil, W. H. (1972) Observations on the histochemistry of egg-shell formation in Lintonium vibex (Trematoda: Fellodistomatidae) Zeitschrift für Parasitenkunde, 39, 195–199.CrossRefGoogle Scholar
Eklu-Natey, D. T., Swiderski, Z., Moczen, T. & Huggel, H. (1982) Ultrastructure of Mehlis' gland and ootype in Schistosoma haematobium (Bilhartz 1851). Tenth International Congress of Electron Microscopy, 3, 609–61.Google Scholar
Erasmus, D. A. (1973) A comparative study of the reproductive sytem of mature, immature and ‘unisexual’ female Schistosoma mansoni. Parasitology, 67, 165–183.CrossRefGoogle Scholar
Erasmus, D. A. (1975) Schistosoma mansoni: Development of the vitelline cell, its role in drug sequestration, and changes induced by Astiban. Experimental Parasitology, 38, 240–256.Google Scholar
Erasmus, D. A. & Davies, T. W. (1979) Schistosoma mansoni and Schistosoma haematobium: Calcium metabolism of the vitelline cell. Experimental Parasitology, 47, 91–106.CrossRefGoogle ScholarPubMed
Erasmus, D. A. & Popiel, I. (1980) Schistosoma mansoni: Drug induced changes in the cell population of the vitelline gland. Experimental Parasitology, 50, 171–187.CrossRefGoogle ScholarPubMed
Erasmus, D. A., Popiel, I. & Shaw, J. R. (1982) A comparative study of the vitelline cell in Schistosoma mansoni, Schistosoma haematobium andSchistosoma mattheei. Parasitology, 84, 283–287.CrossRefGoogle ScholarPubMed
Grant, W. C., Harkema, R. & Muse, K. E. (1977) Ultrastructure of Pharyngostomoides procyonis (Diplostomatidae) Part 2. The female reproductive system. Journal of Parasitology, 63, 1019–1030.Google Scholar
Gresson, R. A. R. (1964) Oogenesis in the hermaphroditic Digenea (Trematoda). Parasitology, 54, 409–421.Google Scholar
Guraya, S. S. (1982) Recent progress in the structure, origin, composition and function of the cortical granules in animal eggs. International Review of Cytology, 78, 257–356.Google Scholar
Henneguy, L. F. (1906) Recherches sur le mode de formation de L'oeuf ectolectithe du Distomum hepaticum. Archives d'Anatomie Microscopique et de Morphologic Experimentale, 9, 47–87.Google Scholar
Irwin, S. (1970) An electron microscope study of Mehlis' gland and egg-shell formation in Fasciola hepatica. Journal of Parasitology, 56, Abstract 4, section 2.Google Scholar
Irwin, S. W. B. & Threadgold, L. T. (1972) Electron microscope studies of Fasciola hepatica X. Egg formation. Experimental Parasitology, 31, 321–331.Google Scholar
Kamson, O. A. (1976) Histological and ultrastructural studies on male Schistosoma mansoni. PhD Thesis University of Wales.Google Scholar
Leitch, B. & Probert, A. J. (1990) Schistosoma haematobium: The effect of Astiban on the cell composition and ultrastructure of the vitelline gland and the ultrastructure of the tegument and gastrodermis. Journal of Helminthology, 64, 65–69.Google Scholar
Leuckart, R. (1886) Die parasiten des menschen und die von ihner herruhrenden krankheiten 2 Aufl. Bd. 1, 2. Abt., S. 230–232. Leipzig: winteresche Verlagshandlung 1886–1901.Google Scholar
Moore, D. V., Yolles, T. K. & Meleney, H. E. (1949) A comparison of common laboratory animals as experimental hosts for Schistosoma mansoni. Journal of Parasitology, 53, 156–170.Google Scholar
Nollen, P. M., Floyd, R. D., Koltzow, R. G. & Deters, D. L. (1976) The timing of reproductive cell development and movement in Schistosoma mansoni, S. japonicum and S. haematobium using techniques of autoradiography and transplantation. Journal of Parasitology, 62, 227–231.Google Scholar
Probert, A. J. & Awad, A. H. H. (1987) Scanning electron microscopy of the tegument of adult S. margrebowiei Le Roux 1933 with particular reference to the structure of the tubercles. Parasitology, 95, 491–498.Google Scholar
Rao, H. K. (1959) Observations on the Mehlis' gland complex in the liver fluke. Fasciola hepatica. Journal of Parasitology, 45, 347–351.CrossRefGoogle ScholarPubMed
RAO, H. K. (1963) The structure of the Mehlis' gland complex and histochemistry of Artyfechinostomum mehrai (Farqui) (Trematoda: Echinostomatidae) from a girl at Andra Pradesh, India. Parasitology, 53, 1–5.Google Scholar
Roberts, J. E. (1986) Schistosoma mansoni: studies on the development of the female reproductive system. PhD Thesis University of Wales.Google Scholar
Smithers, S. R. & Terry, R. J. (1965) The infection of laboratory hosts with cercariae of Schistosoma mansoni and the recovery of the adult worms. Parasitology, 55, 695–700.CrossRefGoogle ScholarPubMed
Spence, I. M. & Silk, M. H. (1970) Ultrastructural studies of the blood fluke Schistosoma mansoni. IV. The digestive system. South African Journal of Medical Science, 35, 93–112.Google ScholarPubMed
Spence, I. M. & Silk, M. H. (1971a) Ultrastructural studies of the blood fluke Schistosoma mansoni. V. The female reproductive system—A preliminary report. South African Journal of Medical Science, 36, 41–50.Google Scholar
Spence, I. M. & Silk, M. H. (1971b) Ultrastructural studies of the blood fluke Schistosoma mansoni. VI. The Mehlis' gland. South African Journal of Medical Science, 36, 69–76.Google ScholarPubMed
Thulin, J. (1982) Structure and function of the female reproductive ducts in the fish blood fluke Aporocotyle simplex Odhner 1900 (Digenea Sanguinicolidae). Sarsia, 67, 227–248.Google Scholar