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Responses of inbred mouse strains to infection with intestinal nematodes

  • M.S. Dehlawi (a1) and P.K. Goyal (a2)

Abstract

Comparisons were made of the immune and inflammatory responses of four strains of inbred mice to infection with the intestinal nematodes Trichinella spiralis and Nippostrongylus brasiliensis to determine whether genetically determined ‘high responsiveness’ to infection, seen most clearly in intestinal responses, is independent of the parasite concerned and necessarily correlated with protection. The time course of infection was followed by counting adult worms at intervals after infection. Mucosal mast cells and Paneth cell numbers were determined as indices of the intestinal inflammatory response. Levels of IgG2a and IgG1 antibodies and of the cytokines IFN-γ and IL-5 released from in vitro-stimulated mesenteric node lymphocytes were measured to assess type 1 and type 2 responses. NIH and CBA mice were the most resistant to T. spiralis and N. brasiliensis respectively, resistance in each case being correlated with the most intense intestinal inflammatory responses. C57BL/10 (B10) and B10.BR were the least resistant to T. spiralis, but were as resistant as CBA to N. brasiliensis, despite their intestinal inflammatory responses to both parasites being much lower than the other two strains. Mice infected with T. spiralis made the expected switch from a type 1 (IFN-γ) to a type 2 (IL-5) response between days 2 and 8, and there were no significant differences in levels of these cytokines between the strains. In contrast, when infected with N. brasiliensis, CBA showed an IFN-γ response at day 4, all strains switching to IL-5 by day 8 and NIH mice releasing the greatest amount of IL-5. The results indicate that the ‘high responder’ phenotype to intestinal nematode infection is in part determined by host characteristics, but is also determined by the parasite concerned – seen most clearly by the differences between NIH and CBA when infected with T. spiralis and N. brasiliensis. The fact that ‘low responder’ B10 background mice were more resistant to N. brasiliensis than ‘high responder’ NIH implies that each parasite elicits a particular pattern of protective host responses, rather than parasites being differentially susceptible to the same response profile.

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Corresponding author

* Author and address for correspondence: Department of Immunology, Groote Schuur Hospital, Old Main Building H47, Observatory 7924, Cape Town, South Africa E-mail: pkgoyal50@yahoo.com

References

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Alizadeh, H. & Wakelin, D. (1983) Genetic factors controlling the intestinal mast cell response in mice infected with Trichinella spiralis. Clinical and Experimental Immunology 49, 331337.
Artis, D. & Grencis, R.K. (2001) T helper cell cytokine responses during intestinal nematode infection: induction, regulation and effector function. pp. 331371in Kennedy, M.W. & Harnett, W.(Eds) Parasitic nematodes. Molecular biology, biochemistry and immunology. Wallingford, Oxon, CABI Publishing.
Dehlawi, M.S. & Wakelin, D. (2002) Parameters of intestinal inflammation in mice given graded infections of the nematode Trichinella spiralis. Journal of Helminthology 76, 113117.
Faulkner, H.F., Humphreys, N.H., Renauld, J.C., Van Snick, J. & Grencis, R.K. (1997) Interleukin-9 is involved in host-protective immunity to intestinal nematode infection. European Journal of Immunology 27, 25362540.
Finkelman, F.D., Shea-Donohue, T., Goldhill, J., Sullivan, C.A., Morris, S.C., Madden, K.B., Gause, W.C. & Urban, J.F. (1997) Cytokine regulation of host defense against parasitic gastrointestinal nematodes: lessons from studies with rodent models. Annual Reviews of Immunology 15, 505534.
Goyal, P.K., Wheatcroft, J. & Wakelin, D. (2002) Tyvelose and protective responses to the intestinal stages of Trichinella spiralis. Parasitology International 51, 9198.
Hermanek, J.P., Goyal, P.K. & Wakelin, D. (1994) Lymphocyte, antibody and cytokine responses during concurrent infections between helminths that selectively promote T helper 1 or T helper 2 activity. Parasite Immunology 16, 111117.
Ishikawa, N., Goyal, P.K., Mahida, Y.R., Li, K-F & Wakelin, D. (1998) Early cytokine responses during intestinal parasitic infections. Immunology 93, 257263.
Kamal, M., Wakelin, D., Oulette, A.J., Smith, A., Podolsky, D.K. & Mahida, Y.R. (2001) Mucosal T cells regulate Paneth and intermediate cell numbers in the small intestine of Trichinella spiralis-infected mice. Clinical and Experimental Immunology 126, 117125.
Lawrence, C.E., Kennedy, M.W. & Garside, P. (2001) Gut immunopathology in helminth infections–paradigm lost. pp. 373397in Kennedy, M.W. & Harnett, W.(Eds) Parasitic nematodes. Molecular biology, biochemistry and immunology. Wallingford, Oxon, CABI Publishing.
Lee, T.D.G. & Wakelin, D. (1982) The use of host strain variation to assess the significance of mucosal mast cells in the spontaneous cure response of mice to the nematode Trichuris muris. International Archives of Allergy and Applied Immunology 67, 302305.
Madden, K.B., Urban, J.F., Zitener, H.J., Schrader, J.W., Finkelman, F.D. & Katona, I. (1991) Antibodies to IL-3 and IL-4 suppress helminth-induced intestinal mastocytosis. Journal of Immunology 147, 13871392.
Robinson, K., Bellaby, T. & Wakelin, D. (1995) Immune response profiles in vaccinated and non-vaccinated high- and low-responder mice during infection with the intestinal nematode Trichinella spiralis. Parasitology 110, 7178.
Robinson, M., Wahid, F.N., Behnke, J.M. & Gilbert, F.S. (1989) Immunological relationships during primary infection with Heligmosomoides polygyrus (Nematospiroides dubius): dose-dependent expulsion of adult worms. Parasitology 98, 115124.
Urban, J.F., Noben-Trauth, N., Donaldson, D.D., Madden, K.B., Morris, S.C. & Finkleman, F.D. (1998) IL-13, IL-4R alpha and Stat6 are required for the expulsion of the gastrointestinal nematode parasite Nippostrongylus brasiliensis. Immunity 8, 255264.
Urban, J.F., Schopf, L., Morris, S.C., Orekhova, T., Madden, K.B., Betts, C.J., Gamble, H.R., Byrd, C., Donaldson, D., Else, K.J. & Finkleman, F.D. (2000) Stat 6 signaling promotes protective immunity against Trichinella spiralis through a mast cell- and T cell-dependent mechanism. Journal of Immunology 164, 20462052.
Wahid, F.N. & Behnke, J.M. (1993) Immunological relationships during primary infection with Heligmosomoides polygyrus. Regulation of fast responder phenotype by H-2 and non-H-2 genes. Parasitology 107, 343350.
Wakelin, D. (1980) Genetic control of immunity to parasites. Infection with Trichinella spiralis in inbred and congenic mice showing rapid and slow responses to infection. Parasite Immunology 2, 8598.
Wakelin, D. (1988) Helminth infections. pp. 153224in Wakelin, D. & Blackwell, J.M.(Eds) Genetics of resistance to bacterial and parasitic infection. London, Taylor & Francis.
Wakelin, D. & Donachie, A.M. (1983) Genetic control of eosinophilia. Mouse strain variation in response to antigens of parasite origin. Clinical and Experimental Immunology 51, 239246.
Wakelin, D. & Lloyd, M. (1976) Immunity to primary and challenge infections of Trichinella spiralis in mice: a re-examination of conventional parameters. Parasitology 72, 173182.
Wassom, D.L., Brooks, B.O., Cypess, R.H. & David, C.S. (1983) A survey of susceptibility to infection with Trichinella spiralis of inbred mouse strains sharing common H-2 alleles but different genetic backgrounds. Journal of Parasitology 69, 10331037.

Responses of inbred mouse strains to infection with intestinal nematodes

  • M.S. Dehlawi (a1) and P.K. Goyal (a2)

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