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Recognition of deglycosylated larval proteins of Gnathostoma spinigerum by a monoclonal antibody and human gnathostomiasis antiserum

  • P. Uparanukraw (a1), T. Harnnoi (a1), A. Dantrakool (a1), N. Morakote (a1) and W. Chaicumpa (a2)...


The study on the recognition of 35S-labelled somatic antigens of Gnathostoma spinigerum advanced third-stage larva (aL3) has revealed that the mAb GN6/24 immunoprecipitated 26- and 24-kDa proteins from the undigested and N-glycosidase F-digested larval extracts, respectively. The recognition of the deglycosylated form of the glycoprotein indicated that the mAb reacted with the peptide epitope on the 26-kDa protein. Human gnathostomiasis antiserum immunoprecipitated most of the N-glycosidase F-digested larval proteins including the deglycosylated 26-kDa protein.


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Anantaphruti, M.T. (1989) ELISA for diagnosis of gnatho-stomiasis using antigens from Gnathostoma doloresi and G. spinigerum. Southeast Asian Journal of Tropical Medicine and Public Health 20, 297304.
Boongird, P., Phuapradit, P., Siridej, N., Chirachariyavej, T., Chuahirun, S. & Vejjajiva, A. (1977) Neurological manifestations of gnathostomiasis. Journal of Neurological Sciences 15, 9297.
Chaicumpa, W., Ruangkunaporn, Y., Nopparatana, C., Chongsa-Nguan, M., Tapchaisri, P. & Setasuban, P. (1991) Monoclonal antibody to a diagnostic Mr 24,000 antigen of Gnathostoma spinigerum. International Journal for Parasitology 21, 735738.
Daengsavang, S. (1980) A monograph on the genus Gnatho-stoma and gnathostomiasis in Thailand. Tokyo, Southeast Asian Medical Information Center.
Dharmkrong-At, A., Migasena, S., Suntharasamai, P., Bunnag, D., Priwan, R. & Sirisinha, S. (1986) Enzyme-linked immunosorbent assay for detection of antibody to Gnathostoma antigen in patients with intermittent cutaneous migratory swelling. Journal of Clinical Microbiology 23, 847851.
Maleewong, W., Morakote, N., Thamasonthi, W., Charuchinda, K., Tesana, S. & Khamboonruang, C. (1988) Serodiagnosis of human gnathostomiasis. Southeast Asian Journal of Tropical Medicine and Public Health 19, 201205.
Maleewong, W., Intapan, P.M., Khempila, J., Wongwajana, S., Wongkham, C. & Morakote, N. (1995) Gnathostoma spinigerum: growth and development of third-stage larvae in vitro. Journal of Parasitology 81, 800803.
Nopparatana, C., Setasuban, P., Chaicumpa, W. & Tapchaisri, P. (1991) Purification of Gnathostoma spinigerum specific antigen and immunodiagnosis of human gnathostomiasis. International Journal for Parasitology 21, 677687.
Punyagupta, S., Bunnag, T. & Juttijudata, P. (1990) Eosinophilic meningitis in Thailand: clinical and epidemiological characteristics of 162 patients with myeloencephalitis probably caused by Gnathostoma spinigerum. Journal of Neurological Sciences 51, 8087.
Rusnak, J.M. & Lucey, D.R. (1993) Clinical gnathostomiasis: case report and review of the English-language literature. Clinical Infectious Diseases 16, 3350.
Suntharasamai, P., Desakorn, V., Migasena, S., Bunnag, D & Harinasuta, T. (1985) ELISA for immunodiagnosis of human gnathostomiasis. Southeast Asian. Journal of Tropical Medicine and Public Health 16, 274279.
Tapchaisri, P., Nopparatana, C., Chaicumpa, W. & Setasubun, P. (1991) Specific antigen of Gnathostoma spinigerum for immunodiagnosis of human gnathostomiasis. International Journal for Parasitology 21, 315319.
Teekhasaenee, C., Ritch, R. & Kanchanaranya, C. (1986) Ocular parasitic infection in Thailand. Review of Infectious Diseases 8, 350356.
Tuntipopipat, S., Chawengkirttikul, R. & Sirisinha, S. (1993) A simplified method for the fractionation of Gnathostoma-specific antigens for serodiagnosis of human gnathostomiasis. Journal of Helminthology 67, 297304.


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