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Recognition of deglycosylated larval proteins of Gnathostoma spinigerum by a monoclonal antibody and human gnathostomiasis antiserum

  • P. Uparanukraw (a1), T. Harnnoi (a1), A. Dantrakool (a1), N. Morakote (a1) and W. Chaicumpa (a2)...

Abstract

The study on the recognition of 35S-labelled somatic antigens of Gnathostoma spinigerum advanced third-stage larva (aL3) has revealed that the mAb GN6/24 immunoprecipitated 26- and 24-kDa proteins from the undigested and N-glycosidase F-digested larval extracts, respectively. The recognition of the deglycosylated form of the glycoprotein indicated that the mAb reacted with the peptide epitope on the 26-kDa protein. Human gnathostomiasis antiserum immunoprecipitated most of the N-glycosidase F-digested larval proteins including the deglycosylated 26-kDa protein.

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Corresponding author

*Fax: 6653 217144 E-mail: puparanu@sd01.med.cmu.ac.th

References

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