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Proteolytic activity in Caenorhabditis elegans : soluble and insoluble fractions

  • C. Gimenez-Pardo (a1), C. Vazquez-Lopez, C. de Armas-Serra and F. Rodriguez-Caabeiro

Abstract

Proteolytic activities of soluble and insoluble fractions of the free-living soil nematode Caenorhabditis elegans were measured across a range of substrates, temperatures and pH conditions. Several protease inhibitors were also tested under these conditions. Results of these studies indicate that proteolytic activity is present in cytosolic (CF) and non-cytosolic (NCF) fractions of C. elegans extracts at every condition of pH, temperature and buffer assayed. On the other hand, the use of different protease inhibitors demonstrated the existence of exo and endoproteases types in CF as well as NCF. Moreover our results show that the use of two protease inhibitor mixed types proposed by several authors are not enough to avoid this lytic activity when homogenates of this nematode are employed in biochemical assays.

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Corresponding author

*Fax: 91 8854636 E-mail: celia.vazquez@uni.alcalá.es

References

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Armas-Serra, C. de., Giménez-Pardo, C., Jiménez-González, A., Bernadina, W.E. & Rodríguez-Caabeiro, F. (1995) Purification and preliminary characterization of a protease from the excretion–secretion products of Trichinella spiralis muscle larvae. Veterinary Parasitology 59, 157168.
Brenner, S. (1974) The genetics of Caenorhabditis elegans. Genetics 77, 7194.
Cox, G.N., Pratt, D., Hageman, R. & Boisvenue, R.J. (1990) Molecular cloning and primary sequence of a cysteine protease expressed by Haemonchus contortus adult worms. Molecular and Biochemical Parasitology 41, 2534.
Criado-Fornelio, A., Armas-Serra, C., de. Giménez-Pardo, C., Casado-Escribano, N., Jiménez-González, A. & Rodríguez-Caabeiro, F. (1992) Proteolytic enzymes from Trichinella spiralis larvae. Veterinary Parasitology 45, 133140.
Cross, K. & Barry, D. (1995) A coat for all seasons: the surface of a model nematode. TRP3:. Research and Funding News from Wellcome Trust 3, 1921.
Epstein, H.F. & Liu, F. (1995) Proteins and protein assemblies. Methods in Cell Biology, 48, 437450.
Kingston, I.B. & Petitt, J. (1990) Structure and expression of Ascaris suum collagen genes: a comparison with Caenorhabditis elegans. Acta Tropica 47(5–6), 283287.
Lackey, A., James, E.R., Sakanari, J.A., Resnick, S.D., Brown, M., Bianco, A.E. & Sakanari, J.H. (1989) Extracellular proteases of Onchocerca. Experimental Parasitology 68, 176185.
Lowry, O.H., Rosebrough, N.J., Farr, A.L. & Randall, R.J. (1951) Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193, 265275.
Peterson, G.L. (1983) Determination of total protein. Methods in Enzymology 91, 95119.
Richer, J.K., Sakanari, J.A., Frank, G.R. & Grieve, R.B. (1992) Dirofilaria immitis: proteases produced by third and fourth-stage larvae. Experimental Parasitology 75, 213222.
Rohrer, S.P., Evans, D.V. & Bergstrom, A. (1990) A membrane associated glutamate binding protein from Caenorhabditis elegans and Haemonchus contortus. Comparative Biochemistry and Physiology 95, 223228.
Salvesen, G. & Nagase, H. (1989) Inhibition of proteolytic activity. pp. 83105 in Benyon, R.J. & Bond, J.S. (Eds) Proteolytic enzymes: a practical approach. IRL Press.
Sarkis, G.J., Kurpiewski, M.R., Ashcom, J.D., Jen-Jacobson, L. & Jacobson, L.A. (1988) Proteases of the nematode Caenorhabditis elegans. Archives of Biochemistry and Biophysics 261, 8090.
Schaeffer, J.M. & Haines, H.W. (1990) Avermectin binding in Caenorhabditis elegans a two-state for the avermectin binding site. Biochemical Pharmacology 38, 23292338.
Ward, S. (1988) Caenorhabditis elegans: a model for parasite nematodes. in England, P.T. & Sher, A.J. (Eds) The biology of parasitism. A molecular and immunological approach. New York, Alan R. Liss, Inc.

Proteolytic activity in Caenorhabditis elegans : soluble and insoluble fractions

  • C. Gimenez-Pardo (a1), C. Vazquez-Lopez, C. de Armas-Serra and F. Rodriguez-Caabeiro

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