Skip to main content Accessibility help
×
Home

Proteoglycans from adult worms of Schistosoma haematobium

  • R.R. Hamed (a1), T.M. Maharem (a2) and A.S. El-Guindy (a1)

Abstract

Different types of proteoglycans (PGs) from adult worms of Schistosoma haematobium, were sequentially extracted using chaotropic agents under associative conditions (0.5 M GnCl), dissociative conditions (4 M GnCl) and detergents (Triton X-100 and SDS). The extracts were designated Fl, F2, F3 and F4, respectively. The highest amount of uronic acid and carbohydrate was detected in the associative extract (Fl) while the highest amount of protein was detected in the SDS extract (F4). Agarose polyacrylamide gel electrophoresis (A-PAGE) indicated the presence of a different PG in each extract with different electrophoretic mobilities. Agarose gel electrophoresis of glycosaminoglycan (GAG) separated from GnCl, associative and dissociative extracts, and the residue suggested the presence of dermatan sulphate in the two extracts and the residue, in addition to a GAG-like material found in the associative extract only. This glycosaminoglycan showed resistance to digestion with all mucopolysaccharidases and nitrous acid treatment. Gel filtration chromatography of associative extract on Sepharose CL-6B indicated the presence of three main uronic acid peaks (P1, P2 and P3). Chondroitin sulphate was the main GAG that could be detected in peak one (P1). Peak two (P2) contains carbohydrate and uronic acid but has no protein or absorbance at 280 nm. P2 has two types of GAGs: dermatan sulphate and a GAG-like material. The role of this PG in helping the adult schistosomes in evading immobilization by the host blood clotting cascade is discussed. Antibodies to peak one and peak two were detected in hamster sera infected with S. haematobium and S. mansoni using the ELISA test. The specificity of peak two was found to be evident in its low cross-reactivity (18.9%) when confronted with S. mansoni infected sera.

Copyright

References

Hide All
Albano, R.M. & Mourano, P.A.S. (1983) Presence of sulfated glycans in ascidian tunic and in the body wall of sea cucumber. Biochimica et Biophysica Acta 760, 192196.
Andreani, D.D. & Gray, C.H. (1956) Serum polysaccharide in diabetes mellitus. Clinica Chimica Acta 1, 715.
Bergwerf, A.A., Van Dam, G.J., Rotmans, J.P., Deelder, A.M., Kamerling, J.P. & Vliegenthart, J.F.G. (1994) The immunologically reactive part of immunopurified circulating anodic antigen from Schistosoma mansoni is a threonine-linked polysaccharide consisting of →6)-(β-D-glcpA-(1→3))-β-D-GalpNAc-(1→repeating units. Journal of Biological Chemistry 269, 3151031517.
Bitter, T. & Muir, H.M. (1962) A modified uronic carbazole reaction. Analytical Biochemistry 4, 330334.
Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248254.
Carney, S.L. (1986) Proteoglycans. pp. 97141in Chapplin, M.F. & Kennedy, J.F. (Eds) Carbohydrate analysis. Oxford, Washington DC, IRL Press.
Cassaro, C.M. & Dietrich, C.P. (1977) Distribution of sulfated mucopolysaccharides in invertebrates. Journal of Biological Chemistry 252, 22542261.
Cooper, N.R. (1985) The classical complement pathway activation and regulation of the first complement component. Advances in Immunology 37, 151216.
Damle, S.P., Kieras, F.J., Tzeng, W. & Gregory, J.D. (1979) Isolation and characterization of proteochondroitin sulfate from pig skin. Journal of Biological Chemistry 254, 16141620.
Dunne, D.W. (1990) Schistosome carbohydrates. Parasitology Today 6 (2), 4548.
Engvall, E. & Berlman, P. (1971) Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin-G. Immunochemistry 8, 781784.
Gowda, D.C., Bhavanandon, V.P. & Davidson, E.A. (1986) Isolation and characterization of proteoglycans secreted by normal and malignant human mammary cells. Journal of Biological Chemistry 261, 49264934.
Hockley, D.J. & McLaren, D.J. (1973) Schistosoma mansoni: Changes in the outer membrane of the tegument during development from cercariae to adult worms. International Journal for Parasitology 3, 1325.
Hook, M., Kjellen, L., Johansson, S. & Robinson, J. (1984) Cell surface glycosaminoglycans. Annual Revieiv of Biochemistry 53, 847869.
Horton, D. & Philips, K.D. (1973) Nitrous acid deamintion of glycosides and acetates of 2-amino-2- deoxy-D-glucose. Carbohydrate Research 30, 367374.
Jackson, R.L., Busch, S.J. & Cardin, A.D. (1991) Glycosaminoglycans: molecular properties, protein interactions, and role in physiological processes. Physiological Reviews 71, 481539.
Kelly, C., Payares, G., Simpson, A.J.G. & Smithers, S.R. (1987) Surface and species-specific antigens of S. haematobium. Parasitology 95, 253266.
Kjellen, L. & Lindahl, U. (1991) Proteoglycans: Structures and interactions. Annual Review of Biochemistry 60, 443475.
Kojima, T., Cataldo, W.L., Marchildon, G.A., Marcum, J.A. & Rosenberg, R.D. (1992) Isolation and characterization of heparan sulfate proteoglycans produced by the cloned rat microvascular endothelial cells. Journal of Biological Chemistry 267, 48594869.
Lagunoff, D. & Warren, G. (1962) Determination of 2-deoxy-2-sulfamatoaminohexose content of mucopolysacchrides. Archives of Biochemistry and Biophysics 99, 396403.
McDevitt, C.A. & Muir, H. (1971) Gel electrophoresis of proteoglycans and glycosaminoglycans on large pore composite polyacrylamide-agarose gels. Analytical Biochemistry 44, 612622.
Nash, T.E., Prescott, B. & Neva, F.A. (1974) The characteristics of a circulating antigen. Journal of Immunology 112, 15001508.
Nash, T.E., Nasir-Ud-Din, & Jeanloz, R.W. (1977) Further purification and characterization of circulating antigen in schistosomiasis. Journal of Immunology 119, 16271634.
Omer-Ali, P., Smithers, S.R., Bickle, Q., Philips, S.M., Horn, D. & Simpson, A.J.G. (1988) Analysis of the anti-Schistosoma mansoni surface antibody response during murine infection and its contribution to protective immunity. Journal of Immunology 140, 32733279.
Poole, A.R., Webber, C., Reiner, A. & Roughley, P.J. (1989) Studies of monoclonal antibody to skeletal keratan sulfate. Biochemical Journal 260, 849856.
Robertson, N.P. & Cain, G.D. (1985) Isolation and characterization of glycosaminoglycans from S. mansoni. Comparative Biochemistry and Physiology 82, 299306.
Robertson, N.P., Oaks, J.A. & Cain, G.D. (1984) Biochemical characterization of polysaccharides of the eggs and adults of Hymenolepis diminuta. Molecular and Biochemical Parasitology 10, 99109.
Simpson, A.J.G., Correa-Oliveira, R., Smithers, S.R. & Sher, A. (1983) The exposed carbohydrates of schistosomula of Schistosoma mansoni and their modification during maturation in vivo. Molecular and Biochemical Parasitology 8, 191205.
Tsang, V.C.W., Hubbard, W.J. & Damian, R.T. (1977) Coagulation factor XIIa (activated Hageman Factor) inhibitor from adult S. mansoni. American Journal of Tropical Medicine and Hygiene 26, 243247.
Van Dam, G.J., Seino, J., Rotman, J.P., Daha, M.R. & Deelder, A.M. (1993) Schistosoma mansoni circulating anoding antigen but not circulatory cathoding antigen interacts with complement C1q. European Immunology 23, 28072812.

Proteoglycans from adult worms of Schistosoma haematobium

  • R.R. Hamed (a1), T.M. Maharem (a2) and A.S. El-Guindy (a1)

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed