Skip to main content Accessibility help
×
Home

The predatory capability of three nematophagous fungi in the control of Haemonchus contortus infective larvae in ovine faeces

  • J. Flores-Crespo (a1), D. Herrera-Rodríguez (a1), P. Mendoza de Gives (a1), E. Liébano-Hernández (a1), V.M. Vázquez-Prats (a1) and M.E. López-Arellano (a1)...

Abstract

The effect of oral administration of three different nematode-trapping fungi, in aqueous suspension containing either Dactylaria sp. or Arthrobotrys oligospora conidia or Duddingtonia flagrans chlamydospores, on the number of Haemonchus contortus infective larvae in sheep faeces, was evaluated. The three selected species of fungi produce three-dimensional adhesive nets in the presence of nematodes. Sixteen Creole sheep were divided into four groups of four animals each. Groups 1 and 2 were orally drenched with a suspension containing 2×107 conidia of either A. oligospora or Dactylaria sp. Group 3, received a similar treatment, with D. flagrans chlamydospores, instead of conidia, being administered, at the same dose. Group 4 acted as control, without any fungi. Faecal samples were collected directly from the rectum of each sheep and faecal cultures were prepared and incubated at 15 and 21 days. Larvae were recovered from faecal cultures and counted. The highest reduction of the nematode population occurred in the D. flagrans group, reaching reductions of 96.3% and 91.4% in individual samplings in plates incubated for 15 and 21 days, respectively. Arthrobotrys oligospora showed moderate reductions in the faecal larval population, ranging between 25–64% at 15 days incubation. In general, Dactylaria sp., was less efficient in its trapping ability. Despite the inconsistent results with Dactylaria sp., reduction percentages of 73.4% and 80.7% were recorded in individual samplings during the first and second days, in plates incubated for 15 days. Duddingtonia flagrans, was shown to be a potential biological control agent of H. contortus infective larvae.

Copyright

Corresponding author

*Fax: 01777 320 55 44 E-mail: pedromdgives@hotmail.com

References

Hide All
Araújo, J.V., Santos, M.A., Ferraz, S. & Maia, A.S. (1993) Antagonistic effect of predaceous Arthrobotrys fungi on infective Haemonchus placei larvae. Journal of Helminthology 7, 136138.
Barron, G.L. (1977) The nematode destroying fungi. 140 pp. Guelph, Canada, Canadian Biological Publications Ltd.
Bisset, S.A., Vlasoff, C., West, J. & Morrison, L. (1997) Epidemiology of nematodosis in Romney lambs selectively bred for resistance or susceptibility to nematode infection. Veterinary Parasitology 70, 255259.
Charleston, T. (1994) Control of gastrointestinal parasites in beef production systems. Veterinary Continuing Education, Massey University, No. 159, 157174.
Cheville, F.N. (1988) Introduction to veterinary pathology. Ames Iowa, University Press.
Faedo, M., Larsen, M. & Waller, J.P. (1997) The potential of nematophagous fungi to control the free-living stages of nematode parasites of sheep: comparison between Australian isolates of Arthrobotrys spp. and Duddingtonia flagrans. Veterinary Parasitology 72, 149155.
Faedo, M., Barnes, E.H., Dobson, R.J. & Waller, J.P. (1998) The potential of nematophagous fungi to control the free-living stages of nematode parasites of sheep: pasture plot study with Duddingtonia flagrans. Veterinary Parasitology 76, 129135.
Flores Crespo, J., Herrera Rodríguez, D., Vázquez Prats, V., Flores Crespo, R., Liébano Hernández, E. & Mendoza-De Gives, P. (1999) Acción depredadora in vitro de ocho aislados de hongos contra el nematodo Panagrellus redivivus. Revista Latinoamericana de Microbiología 41, 239244.
Flores-Crespo, J., Mendoza-de Gives, P., Herrera Rodríguez, D., Liébano Hernández, E., Vázquez Prats, V. & Clark, S.J. (2001) Comparison of the use of two nematode-trapping fungi in the control of Haemonchus contortus infective larvae in ovine faeces. International Journal of Nematology 11, 253259.
Githigia, S.M., Kimoro, C.O., Mwangi, D.M. & Gichanga, E.J. (1995) Prevalence and economic significance of Oesophagostomum and other helminths: survey in selected abattoirs around Nairobi, Kenya. Bulletin of Animal Health and Production in Africa 43, 2933.
González Cruz, M.E., Mendoza de Gives, P. & Quiróz Romero, H. (1998) Comparison of the trapping ability of Arthrobotrys robusta and Monacrosporium gephyropagum on infective larvae of Strongyloides papillosus. Journal of Helminthology 72, 209214.
Grønvold, J., Nansen, P., Henriksen, S.A., Thylin, J. & Wolstrup, J. (1988) The capability of the predacious fungus Arthrobotrys oligospora (Hyphomycetales) to reduce numbers of infective larvae of Ostertagia ostertagi (Trichostrongylidae) in cow pats and herbage during the grazing season in Denmark. Journal of Helminthology 62, 271280.
Grønvold, J., Henriksen, S.A., Nansen, P., Wolstrup, J. & Thylin, J. (1989) Attempts to control infection with Ostertagia ostertagi (Trichostrongylidae) in grazing calves by adding mycelium of the nematode-trapping predacious fungus Arthrobotrys oligospora (Hyphomycetales) to cow pats. Journal of Helminthology 63, 115126.
Grønvold, J., Wolstrup, J., Nansen, P., Henriksen, S.A., Larsen, M. & Bresciani, J. (1993) Biological control of nematode parasites in cattle with nematode-trapping fungi: a survey of Danish studies. Veterinary Parasitology 48, 311325.
Gruner, L., Peloille, M., Sauve, C. & Cortet, J. (1985) Survie et conservation de 1'activité pedratice vis-à-vis de nematodes Trichostrongylides après ingestion par des ovins the trios hyphomycetes predateurs. Comptes Rendus des Seances de la Societé de Biologie, París v. 300, Serie III, n, 14, 525528.
Hurley, D., Aguilar, A., Garibay, J. & Landeros, J. (1981) Técnicas de diseño experimental. Pruebas a posteriori. Diferencia mínima significativa. Prueba de Tukey. México D. F. Centro de Investigación y Estudios Avanzados, Universidad Nacional Autónoma de México. pp. 3753.
Larsen, M. (2000) Prospects for controlling animal parasitic nematodes by predacious microfungi. Parasitology 120, S121S131.
Larsen, M., Wolstrup, J., Henriksen, S.A., Dackman, C., Grønvold, J. & Nansen, P. (1991) In vitro stress selection of nematophagous fungi for biocontrol of parasitic nematodes in ruminants. Journal of Helminthology 65, 193200.
Larsen, M., Wolstrup, J., Henriksen, S.A., Grønvold, J. & Nansen, P. (1992) In vivo passage through calves of nematophagous fungi selected for biocontrol of parasitic nematodes in ruminants. Journal of Helminthology 66, 137141.
Larsen, M., Faedo, M. & Waller, P. (1994) The potential of nematophagous fungi to the control the free-living stages of nematode parasites of sheep: survey for the presence of fungi in fresh faeces of grazing livestock in Australia. Veterinary Parasitology 53, 275281.
Larsen, M., Faedo, M., Waller, P. & Hennesy, D.R. (1998) The potential of nematophagous fungi to control the free-living stages of nematode parasites of sheep: studies with Duddingtonia flagrans. Veterinary Parasitology 76, 121128.
Liébano-Hernández, E. (1989) Cultivo e identificación larvaria de nematodos del tracto gastroentérico. pp. 4071in Ruelas, R.C. & Garfias, R.B.(Eds) Diagnóstico de helmintos y hemoparásitos de rumiantes. Asociación Mexicana de Parasitología Veterinaria, A.C., México, D.F.
Llerandi-Juarez, R.D. & Mendoza-de Gives, P. (1998) Resistance of nematophagous fungi chlamydospores to the digestive processes of sheep in Mexico. Journal of Helminthology 72, 155158.
Medway, W., Prier, E.J. & Wilkinson, S.J. (1980) Patología clínica veterinaria. Unión Tipográfica Editorial México. pp. 224230.
Mendoza-de Gives, P., Zavaleta-Mejia, E., Quiroz-Romero, H., Herrera-Rodriguez, D. & Perdomo-Roldan, F. (1992) Interaction between the nematode-destroying fungus Arthrobotrys robusta (Hyphomycetales) and Haemonchus contortus infective larvae in vitro. Veterinary Parasitology 41, 101107.
Mendoza-de Gives, P., Flores Crespo, J., Herrera Rodríguez, D., Vázquez Prats, V.M., Liébano-Hernández, E. & Ontiveros Fernández, G.E. (1998) Biological control of Haemonchus contortus infective larvae in ovine faeces by administering an oral suspension of Duddingtonia flagrans chlamydospores to sheep. Journal of Helminthology 72, 347347.
Mendoza-De Gives, P., Davies, G.K., Clark, S.J. & Behnke, J.M. (1999) Predatory behaviour of trapping fungi against srf mutants of Caenorhabditis elegans and different plant and animal parasitic nematodes. Parasitology 119, 95104.
Nansen, P. (1986) Induction losses and control of helminths in ruminants of temperate regions. International Journal for Parasitology 17, 425434.
Nansen, P. (1987) Ruminant nematodiasis in temperate climates: some production losses associated with intensive husbandry methods. pp. 6972in Kenning, H.D.W. & Guerrero, J.(Eds) The economic impact of parasitism in cattle. Proceedings of the MSD AGVET Symposium. 19 August, 1987, in association with the XXIII World Veterinary Congress, Montreal, Quebec, Canada.
Pryadko, E.I. & Osipov, P.P. (1986) Trials of nematophagous fungi in field conditions. Biologicheskaya 1, 3033.
Pryadko, E.I., Shol, V.A., Isabaev, M.I. & Shaltaeva, K.B. (1985) Nematophagous fungi as a biological control agent of nematode infections in farm animals, pp. 186201in Gvozdev, E.V.(Ed.) Gel'minty zhivotnykh v ekosystemakh Kazakhstana. Alma-Ata, USSR, “Nauka” Kazakhskoi SSR.
Sauer, S. (1996) Epidemiologie und klinsche auswirkungen von infektionen mit Magen-Darm-Strongyliden bei Schaflammern in Koppelhaltung. 151 pp. Inaugural-Disertation, Fachbereich Veterinarmedizin., Justus-Liebig Universitat, Giessen, Germany.
Síntesis geográfica de Morelos (1981) Coordinación de Servicios Nacionales de Estadística, Geografía e Informática (INEGI). Secreatría de Programación y Presupuesto, México, D.F.
Soulsby, E.J.L. (1987) Parasitología y enfermedades parasitarias de los animales domésticos. Ed. Interamericana 1a edición.
Ulloa, M. & Hanlin, R. (1978) Atlas de micología básica. México, D.F., Editorial Concepto.
Waller, P.J. (1993) Nematophagous fungi prospective biological control agents of animal parasitic nematodes. Parasitology Today 9, 529531.
Waller, P.J. & Faedo, M. (1993) The potential of nematophagous fungi to control the free-living stages of nematode parasites of sheep: screening studies. Veterinary Parasitology 49, 285297.

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed