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A possible relationship between Thromboxane B2 and Leukotriene B4 and the encapsulation of Dirofilaria repens worms in human subcutaneous dirofilariasis

  • R. Morchón (a1), E. Carretón (a2), R. García (a1), T. Zueva (a1), V. Kartashev (a3) and F. Simón (a1)...

Abstract

Human subcutaneous dirofilariosis has several clinical presentations. Many cases present as subcutaneous nodules, as a consequence of a local inflammatory reaction that encapsulates and destroys the worms. In addition, there are cases in which migrating worms located in the ocular area remain unencapsulated. In the present work, the levels of two pro-inflammatory eicosanoids, thromboxane B2 (TxB2) and leukotriene B4 (LTB4) are analysed by commercial Enzime-Linked immunosorbent assay (ELISA) in serum samples from 43 individuals, 28 diagnosed as having subcutaneous dirofilariasis presenting a subcutaneous nodule, five diagnosed as having dirofilariasis, in which the worms remained unencapsulated in the periphery of the eye, and ten healthy individuals living in a non-endemic area, used as controls. The worms were surgically removed, identifying Dirofilaria repens as the causative agent in all cases, by Polymerase Chain Reaction (PCR). Individuals with nodules showed significantly higher levels of TxB2 and LTB4 than healthy controls, whereas significant differences in LTB4 levels were observed between individuals with unencapsulated worms and healthy controls. It is speculated that the absence of LTB4 may contribute to the fact that worms remain unencapsulated as a part of immune evasion mechanisms.

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Corresponding author

Author for correspondence: R. Morchón, E-mail: rmorgar@usal.es

References

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Bogatcheva, NV, Sergeeva, MG, Dudek, SM and Verin, AD (2005) Arachidonic acid cascade in endothelial pathobiology. Microvascular Research 69, 107127.
Brattig, NW (2004) Pathogenesis and host responses in human onchocerciasis: impact of Onchocerca filariae and Wolbachia endobacteria. Microbes and Infection 6, 113128.
Brattig, NW, Schwohl, A, Hoerauf, A and Büttner, DW (2009) Identification of the lipid mediator prostaglandin E2 in tissue immune cells of humans infected with the filaria Onchocerca volvulus. Acta Tropica 112, 231235.
Capelli, G, Genchi, C, Baneth, G, et al. (2018) Recent advances on Dirofilaria repens in dogs and humans in Europe. Parasite & Vectors 11, 663.
Cook-Mills, JM and Deem, TL (2005) Active participation of endothelial cells in inflammation. Journal of Leukocyte Biology 77, 487495.
European Academies Science Advisory Council (EASAC) (2010) Climate change and infectious diseases in Europe. Available at https://www.easac.eu/fileadmin/PDF_s/reports_statements/Climate_change_and_infectious_diseases_in_Europe.pdf (accessed 27 May 2019).
Genchi, C, Kramer, LH and Rivasi, F (2011) Dirofilarial infections in Europe. Vector Borne and Zoonotic Diseases 11, 13071317.
Gioia, G, Lecová, L, Genchi, M, Ferri, E, Genchi, C and Mortarino, M (2010) Highly sensitive multiplex PCR for simultaneous detection and discrimination of Dirofilaria immitis and Dirofilaria repens in canine peripheral blood. Veterinary Parasitology 172, 160163.
González-Miguel, J, Rosario, L, Rota-Nodari, E, Morchón, R and Simón, F (2010a) Identification of immunoreactive proteins of Dirofilaria immitis and D. repens recognized by sera from patient with pulmonary and subcutaneous dirofilariosis. Parasitology Internatational 59, 248256.
González-Miguel, J, Morchón, R, Siles-Lucas, M, Oleaga, A and Simón, F (2010b) Identification of Dirofilaria immitis immunreactive proteins recognized by sera from infected cats using two-dimensional electrophoresis and mass spectrometry. Molecular and Biochemical Parasitology 174, 7882.
Grandi, G, Morchón, R, Kramer, L, Kartashev, V and Simón, F (2008) Wolbachia in Dirofilaria repens, an agent causing human subcutaneous dirofilariasis. Journal of Parasitology 94, 14211423.
Kartashev, V and Simón, F (2018) Migrating Dirofilaria repens. The New England Journal of Medicine 378, e35.
Kartashev, V, Tverdokhlebova, T, Korzan, A, Vedenkov, A, Simón, L, González-Miguel, J, Morchón, R, Siles-Lucas, M and Simón, F (2015) Human subcutaneous/ocular dirofilariosis in Russian Federation and Belarus, 1997-2013. International Journal of Infectious Diseases 33, 209211.
Kramer, LH, Kartashev, VV, Grandi, G, Morchón, R, Nagorni, SA, Karanis, P and Simón, F (2007) Human subcutaneous dirofilariasis, Russia. Emerging Infectious Diseases 13, 150152.
Liu, LX and Weller, PF (1992) Intravascular filarial parasites inhibit platelet aggregation. Role of parasite-derived prostanoids. The Journal of Clinical Investigation 89, 11131120.
Liu, LX, Buhlmann, JE and Weller, PF (1992) Release of prostaglandin E2 by microfilariae of Wuchereria bancrofti and Brugia malayi. The American Journal of Tropical Medicine and Hygiene 46, 520523.
Moore, J, McDermott, L, Price, NC, Kelly, SN and Cooper, A (1999) Sequence-divergent units of the ABA-1 polyprotein array of the nematode Ascaris suum have similar fatty-acid- and retinol-binding properties but different binding-site environments. Biochemical Journal 340, 337343.
Morchón, R, López-Belmonte, J, Rodríguez-Barbero, A and Simón, F (2006) High levels of serum thromboxane B2 are generated during human pulmonary dirofilariasis. Clinical Vaccine Immunology 13, 11751176.
Morchón, R, Rodríguez-Barbero, A, Velasco, S, López-Belmonte, J and Simón, F (2008) Vascular endothelial cell activation by adult Dirofilaria immitis antigens. Parasitology International 57, 441446.
Morchón, R, González-Miguel, J, Carretón, E, Kramer, LH, Velero, L, Montoya-Alonso, JA, Simón, F and Siles-Lucas, M (2014) Proteomic analysis of the somatic and surface compartments from Dirofilaria immitis adult worms. Veterinary Parasitology 203, 144152.
Pampiglione, S, Canestri Trotti, G and Rivasi, F (1995) Human dirofilariasis due to Dirofilaria (Nochtiella) repens: a review of world literature. Parassitologia 37, 149193.
Rogerio, AP and Anibal, FF (2012) Role of leukotrienes on protozoan and helminth infections. Mediators of Inflammation 2012, 595694.
Rola-Pleszcynski, M, Gagnon, L and Sirois, P (1983) Leukotriene B4 augments human natural cytotoxic cell activity. Biochemical and Biophysical Research Communications 113, 531537.
Sankari, T, Hoti, SL, Das, L, Govindaraj, V and Das, PK (2013) Effect of Diethylcarbamazine (DEC) on prostaglandin levels in Wuchereria bancrofti infected microfilaraemics. Parasitology Research 112, 23532359.
Simón, F, Siles-Lucas, M, Morchón, R, González-Miguel, J, Mellado, I, Carretón, E and Montoya-Alonso, JA (2012) Human and animal dirofilariasis: the emergence of a zoonotic mosaic. Clinical Microbiology Reviews 25, 507544.
Tezuka, H, Imai, S, Muto, R, Furuhashi, Y and Fujita, K (2002) Recombinant Dirofilaria immitis polyprotein that stimulates murine B cells to produce nonspecific polyclonal immunoglobulin E antibody. Infection and Immunity 70, 12351244.
Yaqoob, P (2004) Fatty acids and the immune system: from basic science to clinical applications. Proceedings of the Nutrition Society 63, 89104.

Keywords

A possible relationship between Thromboxane B2 and Leukotriene B4 and the encapsulation of Dirofilaria repens worms in human subcutaneous dirofilariasis

  • R. Morchón (a1), E. Carretón (a2), R. García (a1), T. Zueva (a1), V. Kartashev (a3) and F. Simón (a1)...

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