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The occurrence of the filarial nematode Dirofilaria repens in canine hosts from Maio Island, Cape Verde

  • R. Marcos (a1), C. Pereira (a1), J.P. Maia (a2) (a3), M. Santos (a1), C. Luzzago (a4), S. Lauzi (a4), C. Genchi (a4), A. Faustino (a1) and P. Puente-Payo (a1)...

Abstract

The prevalence of canine Dirofilaria infection in Maio Island (Cape Verde) was analysed by serology, morphological and molecular identification of the parasite species. Blood and sera were collected from 150 dogs and 80 cats aged over 6 months from various localities of the island. DNA was extracted from blood and samples were screened by polymerase chain reaction (PCR) using microfilaria-specific primers. No Dirofilaria immitis was found in dogs while D. repens microfilariae were found in 5.3% of dogs and 6% were positive by PCR. The species identity was confirmed by sequencing of PCR products, which showed almost 100% homology with D. repens European sequences published in GenBank. No difference in Dirofilaria infection was observed between males and females or in dogs with different weights. However, older dogs and those from the western part of Maio Island were more frequently infected. No Dirofilaria was found in cats. This study represents the first evidence of D. repens in Cape Verde (West Africa) and highlights the need for implementing control measures and for a better surveillance of dirofilariosis in Africa.

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Altschul, S.F., Gish, W., Miller, W., Myers, E.W. & Lipman, D.J. (1990) Basic local alignment search tool. Journal of Molecular Biology 215, 403410.
Alves, J., Gomes, B., Rodrigues, R., Silva, J., Arez, A.P., Pinto, J. & Sousa, C.A. (2010) Mosquito fauna on the Cape Verde Islands (West Africa): an update on species distribution and a new finding. Journal of Vector Ecology 35, 307312.
Antunes, A.C.L. (2013) Estimation and characterization of the dog and cat population on Maio island, Cape Verde: an integration of household survey data and remote sensing imagery. MSc thesis, Faculty of Veterinary Medicine, University of Lisbon.
Baneth, G., Volansky, Z., Anug, Y., Favia, G., Bain, O., Goldstein, R.E. & Harrus, S. (2002) Dirofilaria repens infection in a dog: diagnosis and treatment with melarsomine and doramectine. Veterinary Parasitology 105, 173178.
Cardoso, L., Mendão, C. & Madeira de Carvalho, L. (2012) Prevalence of Dirofilaria immitis, Ehrlichia canis, Borrelia burgdorferi sensu lato, Anaplasma spp. and Leishmania infantum in apparently healthy and CVBD-suspect dogs in Portugal – a national serological study. Parasites & Vectors 5, 62.
Casiraghi, M., Anderson, T.J., Bandi, C., Bazzocchi, C. & Genchi, C. (2001) A phylogenetic analysis of filarial nematodes: comparison with the phylogeny of Wolbachia endosymbionts. Parasitology 122, 93103.
Casiraghi, M., Bain, O., Guerrero, R., Martin, C., Pocacqua, V., Gardner, S.L., Franceschi, A. & Bandi, C. (2004) Mapping the presence of Wolbachia pipientis on the phylogeny of filarial nematodes: evidence for symbiont loss during evolution. International Journal of Parasitology 34, 191203.
Courtney, C.H. & Cornell, J.A. (1990) Evaluation of heartworm immunodiagnostic tests. Journal of the American Veterinary Medical Association 197, 724729.
Ferri, E., Barbuto, M., Bain, O., Galimberti, A., Uni, S., Guerrero, R., Ferte, H., Bandi, C., Martin, C. & Casiraghi, M. (2009) Integrated taxonomy: traditional approach and DNA barcoding for the identification of filaroid worms and related parasites (Nematoda). Frontiers in Zoology 6, 1.
Garcia, E.M.O. (2010) Gestão integrada dos recursos hídricos da ilha do Maio (Cabo Verde): o contributo das águas subterrâneas. MSc Thesis, Faculty of Sciences, University of Minho.
Genchi, C., Rinaldi, L., Cascone, C., Mortarino, M. & Cringoli, G. (2005) Is heartworm disease really spreading in Europe? Veterinary Parasitology 133, 137148.
Genchi, M., Prego, G. & Genchi, C. (2010) Efficacy of moxidectin microsphere sustained release formulation for the prevention of subcutaneous filarial (Dirofilaria repens) infection in dogs. Veterinary Parasitology 170, 167169.
Genchi, C., Genchi, M., Petry, G., Kruedewagen, E.M. & Schaper, R. (2013) Evaluation of the efficacy of imidacloprid 10%/moxidectin 2.5% (Advocate®, Advantage® Multi, Bayer) for the prevention of Dirofilaria repens infection in dogs. Parasitology Research 112, 8189.
Kirchner, M., Brunner, A., Edelhofer, R. & Joachim, A. (2008) Vector-borne parasites of dogs on the islands of Cabo Verde. Wiener Klinische Wochenschrift 120, 4953.
Liotta, J.L., Sandhu, G.K., Rishniw, M. & Bowman, D. (2013) Differentiation of the microfilariae of Dirofilaria immitis and Dirofilaria repens in stained blood films. Journal of Parasitology 99, 421425.
Maia, J.P., Harris, D.J., Carranza, S. & Gómez-Díaz, E. (2014) A comparison of multiple methods for estimating parasitemia of hemogregarine hemoparasites (Apicomplexa: Adeleorina) and its application for studying infection in natural populations. PloS One 9, e95010.
McCall, J.W., Genchi, C., Kramer, L.H., Guerrero, J. & Venco, L. (2008) Heartworm disease in animals and humans. Advances in Parasitology 66, 193285.
Montoya-Alonso, J.A., Carretón, E., Corbera, J.A., Juste, M.C., Mellado, I., Morchón, R. & Simón, F. (2011) Current prevalence of Dirofilaria immitis in dogs, cats and humans from the Island of Gran Canaria, Spain. Veterinary Parasitology 176, 291294.
Otranto, D., Brianti, E., Dantas-Torres, F., Weigl, S., Latrofa, M.S., Gaglio, G., Cauquil, L., Giannetto, S. & Bain, O. (2011) Morphological and molecular data on the dermal microfilariae of a species of Cercopithifilaria from a dog in Sicily. Veterinary Parasitology 182, 221229.
Pantchev, N., Etzold, M., Daugschies, A. & Dyachenko, V. (2011) Diagnosis of imported canine filarial infections in Germany 2008–2010. Parasitology Research 109, S61S76.
Pascucci, I., Fico, R., DÁngelo, A.R., Serini, S. & Cammà, C. (2007) First notification in Italy of cardiopulmonary filarioses (heartworm disease) in a wolf (Canis lupus). Veterinaria Italiana 43, 851858.
Pereira, P., Almeida, C., Malta, M., Vilaça, R. & Payo-Puente, P. (2013) First report of Dirofilaria immitis in the Republic of Cape Verde. Veterinary Parasitology 192, 290291.
Petry, G., Genchi, M., Schmidt, H., Schaper, R., Lawrenz, B. & Genchi, C. (2015) Evaluation of the adulticidal efficacy of imidacloprid 10%/moxidectin 2.5% (w/v) spot-on (Advocate®, Advantage® Multi) against Dirofilaria repens in experimentally infected dogs. Parasitology Research 114, S131S144.
Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular cloning: a laboratory manual. 545 pp. New York, Cold Spring Harbor Laboratory Press.
Schwan, E.V. (2009) Filariosis of domestic carnivores in Gauteng, KwaZulu-Natal and Mpumalanga provinces, South Africa, and Maputo Province, Mozambique. PhD Thesis, University of Pretoria, South Africa.
Silbermayr, K., Eigner, B., Duscher, G.G., Joachim, A. & Fuehrer, H.P. (2014) The detection of different Dirofilaria species using direct PCR technique. Parasitology Research 113, 513516.
Simón, F., Siles-Lucas, M., Morchón, R., González-Miguel, J., Mellado, I., Carretón, E. & Montoya-Alonso, J. (2012) Human and animal dirofilariasis: the emergence of a zoonotic mosaic. Clinical Microbiology Reviews 25, 507544.
Siwila, J., Mwase, E.T., Nejsum, P. & Simonsen, P. (2015) Filarial infections in domestic dogs in Lusaka, Zambia. Veterinary Parasitology 210, 250254.
Suzuki, J., Kobayashi, S., Okata, U., Matsuzaki, H., Mori, M., Chen, K.R. & Iwata, S. (2015) Molecular analysis of Dirofilaria repens removed from a subcutaneous nodule in a Japanese woman after a tour to Europe. Parasite 22, 21.
Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution 30, 27252759.

The occurrence of the filarial nematode Dirofilaria repens in canine hosts from Maio Island, Cape Verde

  • R. Marcos (a1), C. Pereira (a1), J.P. Maia (a2) (a3), M. Santos (a1), C. Luzzago (a4), S. Lauzi (a4), C. Genchi (a4), A. Faustino (a1) and P. Puente-Payo (a1)...

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