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New species of Parasaccocoelium (Haploporidae) and new genus Pseudohaplosplanchnus (Haplosplanchnidae) from mullet fish in the Far East of Russia and Vietnam: morphological and molecular data

Published online by Cambridge University Press:  14 May 2020

D.M. Atopkin Open the ORCID record for D.M. Atopkin [Opens in a new window] ,
V.V. Besprozvannykh ,
D.N. Ha ,
V.H. Nguyen  and
V.T. Nguyen
D.M. Atopkin*
Affiliation:
Federal Scientific Center of East Asia Terrestrial Biodiversity, Far Eastern Branch of Russian Academy of Sciences, Vladivostok690022, Russia Department of Cell Biology and Genetics, Far Eastern Federal University, Ajax-10 str., Vladivostok690051, Russia
V.V. Besprozvannykh
Affiliation:
Federal Scientific Center of East Asia Terrestrial Biodiversity, Far Eastern Branch of Russian Academy of Sciences, Vladivostok690022, Russia
D.N. Ha
Affiliation:
Institute of Ecology and Biological Resources, Vietnamese Academy of Sciences and Technology, Hanoi, Vietnam
V.H. Nguyen
Affiliation:
Institute of Ecology and Biological Resources, Vietnamese Academy of Sciences and Technology, Hanoi, Vietnam
V.T. Nguyen
Affiliation:
Hai Duong Medical Technical University, Vietnam
*
Author for correspondence: D.M. Atopkin, E-mail: atop82@gmail.com
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Abstract

A description and the molecular characterization of two new species in the Haploporidae and Haplosplanchnidae families are provided herein. Parasaccocoelium armatum n. sp. was collected from the intestine of a Mugil cephalus Linnaeus, 1758 from the Primorsky region, Russia, and Pseudohaplosplanchnus catbaensis n. g. n. sp. was collected from Moolgarda seheli (Forsskål, 1775) in the coastal waters of Cat Ba Island, Vietnam. The morphological features of P. armatum n. sp. closely resemble those of Parasaccocoelium polyovum, but these species differ from one another by hermaphroditic sac and vitellaria area length and by maximal egg size. The main difference between P. armatum n. sp. and P. polyovum is the presence of an armed hermaphroditic duct in the new species. Molecular data support the case for inclusion of the studied trematodes in P. armatum n. sp. Worms P. catbaensis n. g. n. sp. from the mullet from Vietnam are morphologically close to Haplosplanchnus (Haplosplosplanchninae). The only difference between P. catbaensis n. g. n. sp. and species of Haplosplanchnus is the presence of few (1–7) large eggs, measuring 135–142 × 92–104 μm, versus numerous small eggs with a maximal size of 75 × 50 μm. Phylogenetic analysis showed that there is a contradiction between the morphological similarity of the worms and their position in the Haplosplanchnidae system, based on the genetic data. Results of this study indicate that P. catbaensis n. g. n. sp. is genetically distant from other representatives of Haplosplanchnus, despite their morphological similarity. According to the molecular data, P. catbaensis n. g. n. sp. is close to Hymenocotta mulli Manter, 1961 (Hymenocottinae). However, these species are considerably different to each other morphologically. Molecular data argue for the possibility of establishing a new subfamily for P. catbaensis n. g. n. sp. However, considering earlier studies of Haplosplanchnidae, we support the view that creating new subfamilies within this family is unreasonable because of the lack of molecular data for most haplosplanchnid species, which are necessary to resolve the problematic systematics and phylogeny of this family.

Keywords

HaplosplanchnidaeHaploporidaeParasaccocoeliumPseudohaplosplanchnusmulletmolecular dataRussian Far EastVietnamphylogenetic relationships
Type
Research Paper
Information
Journal of Helminthology , Volume 94 , 2020 , e154
Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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