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Morphometric and phylogenetic analyses of Serpentirhabdias viperidicus n. sp. (Nematoda: Rhabdiasidae) from the lancehead snake Bothrops moojeni Hoge, 1966 (Reptilia: Serpentes: Viperidae) in Brazil

  • D.H. Morais (a1), A. Aguiar (a1), M.I. Müller (a1), R.B. Narciso (a1), L.A.F. da Silva (a1) and R.J. da Silva (a1)...


Serpentirhabdias viperidicus n. sp. (Nematoda: Rhabdiasidae) is described from the lungs of the ‘Brazilian lancehead’ Bothrops moojeni (Hoge, 1966) from the savannah in São Paulo State, Brazil. The new species is the eighth species of Serpentirhabdias described in the Neotropical region, and differs from other species mainly by a combination of characters: lips slightly notable, presence of fine striations at posterior ends, presence of two parallel lines with intercalated pores, a pore-shaped phasmid situated at the level of the anal aperture and another two in the posterior half of the tail. It is the first species of Serpentirhabdias reported in this snake host and the second species of this genus found parasitizing South American viperidian snakes. Molecular phylogenetic analysis using ribosomal (ITS and 28S partial) genes confirms Serpentirhabdias viperidicus n. sp. as a new species that clustered in the Serpentirhabdias clade, sister taxon to Serpentirhabdias fuscovenosa and Serpentirhabdias elaphe. This is the first description of Serpentirhabdias species from Brazil using molecular approaches and morphological characters to confirm the monophyly of this recent genus.


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Allison, V., Ubelaker, J., Webster, R. Jr & Riddle, J. (1972) Preparations of helminths for scanning electron microscopy. Journal of Parasitology 58, 414416.
Barrella, T.H., Santos, K.R. & Silva, R.J. (2010) Rhabdias filicaudalis n. sp. (Nematoda: Rhabdiasidae) from the snake Spilotes pullatus (Serpentes: Colubridae) in Brazil. Journal of Helminthology 84, 292296.
Borges, R.C. & Araújo, A.F.B. (1998) Selecão de habitat entre duas espécies de jararaca (Bothrops moojeni Hoge and B. neuwiedi Wagler) (Serpentes: Viperidae). Revista Brasileira de Biologia 58, 591601.
Campbell, J.A. & Lamar, W.W. (1989) The venomous reptiles of Latin America. 1st edn. 440 pp. Ithaca, Comstock Publishing Associates.
Carrasco, P.A., Mattoni, C.I., Leynaud, G.C. & Scrocchi, G.J. (2012) Morphology, phylogeny and taxonomy of South American bothropoid pitvipers (Serpentes, Viperidae). Zoologica Scripta 41, 109124.
Cipriani, P., Mattiucci, S., Paoletti, M., Santoro, M. & Nascetti, G. (2012) Rhabdias esculentarum n. sp. (Nematoda: Rhabdiasidae) from green frogs of the Rana esculenta species complex in Italy: molecular evidence, morphological description and genetic differentiation from its congeners in frogs and toads. Systematic Parasitology 82, 131146.
Costa, H.C. & Bernis, R.S. (Org.) (2014) Brazilian reptiles – list of species. Electronic database available at (accessed 20 November 2015).
Dare, O.K., Nadler, S.A. & Forbes, M.R. (2008) Nematode lungworms of two new species of anuran amphibians: evidence for co-adaptation. International Journal for Parasitology 38, 17291736.
Drummond, A.J., Suchard, M.A., Xie, D. & Rambaut, A. (2012) Bayesian phylogenetics with BEAUti and the BEAST 1.7. Molecular Biology and Evolution 29, 19691973.
Duellman, W.E. (1978) The biology of an equatorial herpetofauna in amazonian Ecuador. Miscellaneous publication – University of Kansas, Museum of Natural History 65, 1352.
Fenker, J., Tedeschi, L., Pyron, R.A. & Nogueira, C.N. (2014) Phylogenetic diversity, habitat loss and conservation priorities in South American pitvipers (Crotalinae: Bothrops and Bothrocophias). Diversity and Distributions 20, 11081119 (supporting information: Appendix S1, Appendix S2, Appendix S3).
Folmer, O., Black, M., Hoeck, W., Lutz, R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3, 294299.
Guindon, S., Dufayard, J.F., Lefort, V., Anisimova, M., Hosdijk, W. & Gascuel, O. (2010) New algorithms and methods to estimate Maximum-Likelihood Phylogenies: assessing the performance of PhyML 3.0. Systematic Biology 59, 307321.
Henderson, R.W., Dixon, J.R. & Soini, P. (1979) Resource partitioning in amazonian snake communities. Contributions in biology and geology/Milwaukee Public Museum 22, 111.
Kimura, M. (1980) A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16, 111120.
Kuzmin, Y. (2013) Review of Rhabdiasidae (Nematoda) from the Holarctic. Zootaxa 3639, 176.
Kuzmin, Y. & Tkach, V.V. (2015) Rhabdias. World Wide Web electronic publication. Available at (accessed 10 January 2016).
Kuzmin, Y., Tkach, V.V. & Brooks, D.R. (2007) Rhabdias alabialis sp. nov. and R. pseudosphaerocephala sp. nov. (Nematoda: Rhabdiasidae) in the marine toad, Bufo marinus (Lissamphibia: Anura: Bufonidae) in Central America. Journal of Parasitology 93, 159165.
Kuzmin, Y., Melo, F.T.V. & Santos, J.N. (2014) A new species of Serpentirhabdias Tkach, Kuzmin & Snyder, 2014 (Nematoda: Rhabdiasidae) parasitic in the brown ground snake Atractus major Boulenger (Reptilia: Serpentes: Dipsadidae) in Brazil. Systematic Parasitology 89, 101106.
Kuzmin, Y., Giese, E.G., Melo, F.T.V., Costa, P.A.F.B, Maschio, G.F. & Santos, J.N. (2016) Description of Serpentirhabdias atroxi n. sp. (Nematoda: Rhabdiasidae), a parasite of Brothrops atrox (Linnaeus) (Reptilia: Serpentes: Viperidae) in Brazilian Amazonia. Systematic Parasitology 93, 3745.
Langford, G.J. & Janovy, J. Jr (2013) Host specificity of the North American Rabdias spp. (Nematoda: Rhabdiasidae): combined field data and experimental infections with a molecular phylogeny. Journal of Parasitology 99, 277286.
Larkin, M.A., Blackshields, G., Brown, N.P., Chenna, R., McGettigan, P.A., McWilliam, H., Valentin, F., Wallace, I.M., Wilm, A., Lopez, R., Thompson, J.D., Gibson, T.J. & Higgins, D.G. (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23, 29472948.
Leloup, P. (1984) Various aspects of venomous snake breeding on a large scale. Acta Zoologica et Pathologica Antverpiensia 78, 77198.
Martínez-Salazar, E.A. & León-Régagnon, V. (2006) Rhabdias lamothei n. sp. (Nematoda: Rhabdiasidae) from Leptodeira maculata (Colubridae) in Mexico, including new records of R. fuscovenosa (Railliet, 1899) Goodey, 1924. Zootaxa 1257, 2748.
Nogueira, C., Sawaya, R. & Martins, M. (2003) Ecology of the pitviper, Bothrops moojeni, in the Brazilian Cerrado. Journal of Herpetology 37, 653659.
Pereira, C. (1927) Fauna helminthologica de ophidios brasileiros. Boletim Biologico 35, 179185.
Pereira, C. (1928) Fauna helmintologica dos ophidios brasileiros (2o). Rhabdias vellardi n. sp. Boletim Biologico 11, 1322.
Posada, D. (2008) jModelTest: phylogenetic model averaging. Molecular Biology and Evolution 25, 12531256.
Pyron, A.R, Burbrink, F.T. & Wiens, J.J. (2013) A phylogeny and revised classificaton of Squamata, including 4161 species of lizards and snakes. BioMed Central Evolutionary Biology 13, 153.
Rambaut, A. (2009) Molecular evolution, phylogenetics and epidemiology: Fig-Tree. Available at http// (accessed 10 November 2015).
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. & Kumar, S. (2011) MEGA5: Molecular Evolutionary Genetics Analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution 28, 27312739.
Tkach, V.V., Kuzmin, Y. & Snyder, S.D. (2014) Molecular insight into systematics, host associations, life cycles and geographic distribution of the nematode family Rhabdiasidae. International Journal for Parasitology 44, 273284.
Vicente, J.J., Rodrigues, H.O., Gomes, D.C. & Pinto, R.M. (1991) Nematóides do Brasil 2 parte: nematóides de Anfíbios. Revista Brasileira de Zoologia 7, 549626.
Vitt, L.J. (1987) Communities. pp. 335365 in Seigel, R.A., Collins, J.T. & Novak, S.S. (Eds) Snakes: ecology and evolutionary biology. New York, MacMillan.


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