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Morphometric and molecular analyses of two digenean species from the mullet: Skrjabinolecithum spinosum n. sp. from the Russian southern Far East and Unisaccus tonkini n. sp. from Vietnam

Published online by Cambridge University Press:  17 October 2017

V.V. Besprozvannykh ,
D.M. Atopkin ,
H.D. Ngo ,
N.V. Ha ,
N.V. Tang  and
A.Yu. Beloded
V.V. Besprozvannykh
Affiliation:
Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the Russian Academy of Sciences
D.M. Atopkin*
Affiliation:
Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the Russian Academy of Sciences Department of Cell Biology and Genetics, Far Eastern Federal University, Vladivostok, Russia
H.D. Ngo
Affiliation:
Institute of Ecology and Biodiversity, Vietnamese Academy of Sciences and Technology, Hanoi, Vietnam
N.V. Ha
Affiliation:
Institute of Ecology and Biodiversity, Vietnamese Academy of Sciences and Technology, Hanoi, Vietnam
N.V. Tang
Affiliation:
Institute of Ecology and Biodiversity, Vietnamese Academy of Sciences and Technology, Hanoi, Vietnam
A.Yu. Beloded
Affiliation:
Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the Russian Academy of Sciences
*
Author for correspondence: D.M. Atopkin, Fax: +7 4232310193 E-mail: atop82@gmail.com
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Abstract

Adults of Skrjabinolecithum spinosum n. sp. were discovered in Mugil cephalus from the Gulf of Peter the Great in southern Far-East Russia. Additionally, adults of Unisaccus tonkini n. sp. were found in the intestine of Moolgarda cunnesius and Moolgarda seheli from the coastal waters of Cat Ba Island, Tonkin Bay, northern Vietnam. Skrjabinolecithum spinosum n. sp. possesses a larger body, and ventral and oral sucker size in comparison with Skrjabinolecithum vitellosum, a smaller pharynx size and body length/width rate ratio in comparison to Skrjabinolecithum pyriforme, a smaller body length and prepharynx size in comparison to Skrjabinolecithum lobolecitum and a smaller pharynx length and egg size in comparison to Skrjabinolecithum indicum and S. lobolecitum. The new species also differs from S. indicum, S. lobolecitum and S. vitellosum by the form of the testis, and from the last two species by the presence of a two-branched intestine. The morphometric parameters of S. spinosum n. sp. are similar to those of Skrjabinolecithum spasskii. However, S. spinosum n. sp., unlike S. spasskii, has an armed hermaphroditic duct. Unisaccus tonkini n. sp. is similar to Unisaccus spinosus (Martin, 1973), Unisaccus brisbanensis (Martin, 1973) and Unisaccus overstreeti (Ahmad, 1987) in body size but differs in oral sucker, pharynx and hermaphroditic sac size from U. spinosus, and in ventral sucker and ovary size from U. brisbanensis and U. overstreeti. Bayesian phylogenetic analysis, based on combined data of internal transcribed spacer 2 (ITS2) and partial 28S rRNA gene sequences, confirmed the validity of S. spinosum n. sp. and U. tonkini n. sp. Analysis of interrelationships of the family Haploporidae, including molecular data on new species, showed that the Waretrematinae subfamily is more heterogeneous in comparison with Haploporinae and Forticulcitinae, and includes U. tonkini n. sp.

Type
Research Paper
Information
Journal of Helminthology , Volume 92 , Issue 6 , November 2018 , pp. 713 - 724
Copyright
Copyright © Cambridge University Press 2017 

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