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Morphological and molecular characterization of Strongyloides ophidiae (Nematoda, Strongyloididae)

Published online by Cambridge University Press:  28 August 2009

K.R. dos Santos ,
B.C. Carlos ,
K.S. Paduan ,
S.M. Kadri ,
T.H. Barrella ,
M.R.V. Amarante ,
P.E.M. Ribolla  and
R.J. da Silva
K.R. dos Santos
Affiliation:
Departamento de Medicina Veterinária, Universidade Federal do Piauí, Campus Cinobelina Elvas, Bom Jesus, Piauí, Brazil
B.C. Carlos
Affiliation:
Instituto de Biociências, UNESP, Campus de Botucatu, São Paulo, Brazil
K.S. Paduan
Affiliation:
Instituto de Biociências, UNESP, Campus de Botucatu, São Paulo, Brazil
S.M. Kadri
Affiliation:
Instituto de Biociências, UNESP, Campus de Botucatu, São Paulo, Brazil
T.H. Barrella
Affiliation:
Instituto de Biociências, UNESP, Campus de Botucatu, São Paulo, Brazil
M.R.V. Amarante
Affiliation:
Departamento de Parasitologia, Instituto de Biociências, UNESP, Campus de Botucatu, São Paulo, Brazil
P.E.M. Ribolla
Affiliation:
Departamento de Parasitologia, Instituto de Biociências, UNESP, Campus de Botucatu, São Paulo, Brazil
R.J. da Silva*
Affiliation:
Departamento de Parasitologia, Instituto de Biociências, UNESP, Campus de Botucatu, São Paulo, Brazil
*
*Fax: 55 14 38153744 E-mail: reinaldo@ibb.unesp.br
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Abstract

The aim of the present study is to report morphological data from parasitic female, rhabditoid and filarioid larvae, free-living female worms and eggs of Strongyloides ophidiae (Nematoda, Strongyloididae). In addition, a molecular DNA analysis was carried out using a pool of eight S. ophidiae parasitic females. Samples were obtained from the small intestine of Oxyrhopus guibei (Serpentes, Colubridae) collected in the municipality of Lençóis Paulista, State of São Paulo, Brazil. DNA amplification by polymerase chain reaction (PCR) resulted in a 350 bp band for samples containing S. ophidiae and Strongyloides venezuelensis DNA. Strongyloides ophidiae nucleotide sequence analysis showed 98% similarity with Strongyloides procyonis and 97% with Strongyloides cebus, Strongyloides stercoralis, Strongyloides fuelleborni and Strongyloides sp. from snakes.

Type
Research Papers
Information
Journal of Helminthology , Volume 84 , Issue 2 , June 2010 , pp. 136 - 142
Copyright
Copyright © Cambridge University Press 2009

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