Skip to main content Accessibility help

Morphological and molecular characterization of Megalobatrachonema hainanensis sp. nov. (Nematoda: Ascaridida), with phylogenetic position of Megalobatrachonema in Cosmocercoidea

  • H.-X. Chen (a1), L.-P. Zhang (a1) and L. Li (a1)


The genus Megalobatrachonema is a rare group of nematode parasites within Ascaridida. The systematic status of Megalobatrachonema in the superfamily Cosmocercoidea (Ascaridida) has long been controversial. The relationship of Megalobatrachonema and Chabaudgolvania remains unsolved. In the present study, a new species of Megalobatrachonema, M. hainanensis sp. nov., was described based on specimens collected in Amolops hainanensis (Boulenger) and Hylarana spinulosa (Smith) (Amphibia: Anura) from Hainan Island, China. The large ribosomal DNA (28S) and internal transcribed spacer (ITS1-5.8S-ITS2) were also sequenced for molecular identification and phylogenetic study. Phylogenetic analyses using maximum likelihood (ML) inference and Bayesian inference (BI) based on 28S and ITS1 sequence data, respectively, supported that Megalobatrachonema is a member of the family Kathlaniidae. In addition, the genetic comparison and phylogenetic results based on ITS1 sequence data also supported that the genus Chabaudgolvania should be considered as a synonym of Megalobatrachonema.


Corresponding author

Author for correspondence: L. Li, E-mail:


Hide All
Baker, MR (1980) Reclassification of Oxysomatium inglisi Anderson, 1964 and Aplectana gigantica Olsen, 1938 (Nematoda: Cosmocercoidea) from North American frogs. Systematic Parasitology 1, 245253.
Baker, MR (1986) Redescription of Megalobatrachonema (Chabaudgolvania) elongata (Baird, 1858) n. comb. (Nematoda: Kathlaniidae) parasitic in North American salamanders. Canadian Journal of Zoology 64, 15731575.
Bursey, CR, Goldberg, SR and Kraus, F (2012) A new species of Megalobatrachonema (Nematoda: Kathlaniidae) in Fojia bumui (Squamata: Scincidae) from Papua New Guinea. Journal of Parasitology 98, 973976.
Chabaud, AG (1978) CIH Keys to the nematode parasites of vertebrates. No. 6. Keys to genera of the superfamilies Cosmocercoidea, Seuratoidea, Heterakoidea and Subuluroidea. Farnham Royal: Commonwealth Agricultural Bureaux, 71 pp.
Freitas, JFT (1958) Estudos sôbre “Oxyascarididae” (Travassos, 1920) (Nematoda, Subuluroidea). Memórias do Instituto Oswaldo Cruz 56, 489559.
Hartwich, G (1960) Uber Megalobatrachonema terdentatum (Linstow, 1890) nov. comb. und die Stellung von Megalobatrachonema Yamaguti, 1941 im System der Ascaridina (Nematoda). Zeitschrift Für Parasitenkunde 19, 606616.
Hillis, D and Bull, MJJ (1993) An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis. Systematic Biology 42, 182192.
Kumar, S, Stecher, G and Tamura, K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33, 18701874.
Nadler, SA and Hudspeth, DSS (1998) Ribosomal DNA and phylogeny of the Ascaridoidea (Nemata: Secernentea): implications for morphological evolution and classification. Molecular Phylogenetics and Evolution 10, 221236.
Olsen, OW (1938) Aplectana gigantica (Cosmocercidae), a new species of nematode from Rana pretiosa. Transactions of the American Microscopical Society 57, 200203.
Posada, D and Crandall, KA (2001) Selecting the best-fit model of nucleotide substitution. Systematic Biology 50, 580601.
Richardson, JPM (1988) Phylogenetic Analysis of the Genus Megalobatrachonema Yamaguti, 1941 (Ascaridida: Cosmocercoidea: Kathlaniidae) with Field and Laboratory Observations of M. waldent (MSc thesis). University of British Columbia, Vancouver, Canada.
Richardson, JPM and Adamson, ML (1988a) Megalobatrachonema (Chabaudgolvania) waldeni n. sp. (Nematoda: Kathlaniidae) from the intestine of the northwestern salamander, Ambystoma gracile (Baird). Canadian Journal of Zoology 66, 15021505.
Richardson, JPM and Adamson, ML (1988b) A new Kathlaniidae (Cosmocercoidea; Nematoda), Megalobatrachonema (Chabaudgolvanid) moraveci sp. n. from the intestine of the rough-skinned newt, Taricha granulosa. Proceedings of the Helminthological Society of Washington 55, 155159.
Richardson, JPM and Adamson, ML (1990) Phylogenetic analysis of Megalobatrachonema Yamaguti, 1941 (Ascaridida: Cosmocercoidea: Kathlaniidae): reinstatement of Chabaudgolvania Freitas, 1958. Canadian Journal of Zoology 68, 531538.
Ronquist, F et al. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61, 539542.
Skrjabin, I, Shikhobalovna, P and Lagodovskaey, AA (1964) Essentials of Nematodology: Oxyurata of Animals and Man. Part 3. Vol. 13. (In Russian) Moscow: Academy of Sciences of the USSR, 686 pp. (translated by the Israel Program for Scientific Translations, Jerusalem, 1976.)
Tamura, K et al. (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution 30, 27252729.
Yamaguti, S (1941) Studies on the helminth fauna of Japan. Part 34. Amphibian nematodes II. Japanese Journal of Zoology 9, 397408.
Zhu, X et al. (2000) Assessing sequence variation in the internal transcribed spacers of ribosomal DNA within and among members of the Contracaecum osculatum complex (Nematoda: Ascaridoidea: Anisakidae). Parasitology Research 86, 677683.


Morphological and molecular characterization of Megalobatrachonema hainanensis sp. nov. (Nematoda: Ascaridida), with phylogenetic position of Megalobatrachonema in Cosmocercoidea

  • H.-X. Chen (a1), L.-P. Zhang (a1) and L. Li (a1)


Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed.