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Molecular identification of Anisakis species from Pleuronectiformes off the Portuguese coast

  • J.F. Marques (a1), H.N. Cabral (a1), M. Busi (a2) and S. D'Amelio (a2)


Anisakid nematodes belonging to the Anisakis simplex complex are highly prevalent in several fish species off the coast of Portugal and are an important zoonotic problem in the Iberian Peninsula. Two reproductively isolated sibling species of the Anisakis simplex complex were identified from Pleuronectiformes inhabiting the Portuguese coast using restriction fragment length polymorphism (RFLP). Recombinant genotypes corresponding to presumptive Anisakis simplex sensu stricto and Anisakis pegreffii hybrids were also detected by this technique, as well as the species Anisakis typica. Although 25 species of Pleuronectiformes were investigated, Anisakis spp. larvae were only found in seven: Arnoglossus imperialis, Arnoglossus laterna, Lepidorhombus boscii, Citharus linguatula, Platichthys flesus, Dicologlossa cuneata and Solea senegalensis. The occurrence of hybrids in relatively sedentary fishes such as the Pleuronectiformes suggests that the Portuguese coast may constitute an area of hybridization and, therefore, is of particular interest for the study of the process of hybridization and speciation for these anisakids.


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Abollo, E., D'Amelio, S. & Pascual, S. (2001) Fitness of the marine parasitic nematode Anisakis simplex, s.str. in temperate waters of the NE Atlantic. Diseases of Aquatic Organisms 45, 131139.
Abollo, E., Paggi, L., Pascual, S. & D'Amelio, S. (2003) Occurrence of recombinant genotypes of Anisakis simplex, s.s. and Anisakis pegreffii (Nematoda: Anisakidae) in an area of sympatry. Infection, Genetics and Evolution 3, 175181.
Audicana, M.T., Ansotegui, I.J., Fernández de Corres, L. & Kennedy, M.W. (2002) Anisakis simplex: dangerous dead and alive? Trends in Parasitology 18, 2025.
Berendzen, P.B. & Dimmick, W.W. (2002) Phylogenetic relationships of Pleuronectiformes based on molecular evidence. Copeia 3, 642652.
Berland, B. (1961) Nematodes from some Norwegian marine fishes. Sarsia 2, 150.
Briggs, J.C. (1974) Marine zoogeography, London, McGraw-Hill.
D'Amelio, S., Mathiopoulos, K.D., Santos, C.P., Pugachev, O.N., Webb, S.C., Picanço, M. & Paggi, L. (2000) Genetic markers in ribosomal DNA for the identification of members of the genus Anisakis (Nematoda: Asacaridoidea) defined by polymerase chain reaction-based restriction fragment length polymorphism. International Journal for Parasitology 30, 223226.
de Corres, L.F., Del Pozo, M.D. & Aizpuru, F. (2001) Prevalence of sensitization to Anisakis simplex in three Spanish areas, regarding the rates of fish consumption. Relevance of Anisakis simplex allergy. Alergologia Immunologia Clinica 16, 337346.
Ekman, S. (1953) Zoogeography of the sea. London, Sidgwick & Jackson.
Kuris, A.M., Blaustein, A.R. & Alió, J.J. (1980) Hosts as islands. American Naturalist 116, 570586.
MacArthur, R.H. & Wilson, E.O. (1967) The theory of island biogeography. Monographs in Population Biology, No. 1, Princeton, Princeton University Press.
Mattiucci, S., Nascetti, G., Cianchi, R., Paggi, L., Arduino, P., Margolis, L., Brattey, J., Webb, S., D'Amelio, S., Orecchia, P. & Bullini, L. (1997) Genetic and ecological data on the Anisakis simplex complex, with evidence for a new species (Nematoda, Ascaridoidea, Anisakidae). Journal of Parasitology 83, 401416.
Mattiucci, S., Paggi, L., Nascetti, G., Santos, S.P., Costa, G., Di Beneditto, A.P., Ramos, R., Argyrou, M., Cianchi, R. & Bullini, L. (2002) Genetic markers in the study of Anisakis typica (Diesing, 1860): larval identification and genetic relationships with other species of Anisakis Dujardin, 1845 (Nematoda: Anisakidae). Systematic Parasitology 51, 159170.
Mattiucci, S., Abaunza, P., Ramadori, L. & Nascetti, G. (2004) Genetic identification of Anisakis larvae in European hake from Atlantic and Mediterranean waters for stock recognition. Journal of Fish Biology 65, 495510.
Nascetti, G., Paggi, L., Orecchia, P., Mattiucci, S. & Bullini, L. (1983) Two sibling species within Anisakis simplex (Ascaridida: Anisakidae). Parassitologia 25, 239241.
Nascetti, G., Paggi, L., Orecchia, P., Smith, J., Mattiucci, S. & Bullini, L. (1986) Electrophoretic studies on the Anisakis simplex complex (Ascaridida: Anisakidae) from the Mediterranean and North-east Atlantic. International Journal for Parasitology 16, 633640.
Rosales, M.J., Mascaró, C., Fernandez, C., Luque, F., Moreno, M.S., Parras, L., Cosano, A. & Muñoz, J.R. (1999) Acute intestinal anisakiasis in Spain: a fourth-stage Anisakis simplex larva. Memórias do Instituto Oswaldo Cruz 94, 823826.
Svetovidov, A.N. (1984) Gadidae. pp. 680710in Whitehead, P.J.P., Bauchot, M.L., Hureau, J.C., Nielsen, J. & Tortonese, E.(Eds) Fishes of the North-eastern Atlantic and Mediterranean. Paris, UNESCO.
Zhu, X.Q., Gasser, R.B., Podolska, M. & Chilton, N.B. (1999) Characterization of anisakid nematodes with zoonotic potential by nuclear ribosomal DNA sequences. International Journal for Parasitology 28, 19111921.
Zhu, X.Q., D'Amelio, S., Paggi, L. & Gasser, R.B. (2000) Assessing sequence variation in the internal transcribed spacers of ribosomal DNA within and among members of the Contracaecum osculatum complex (Nematoda: Ascaridoidea: Anisakidae). Parasitology Research 86, 677683.

Molecular identification of Anisakis species from Pleuronectiformes off the Portuguese coast

  • J.F. Marques (a1), H.N. Cabral (a1), M. Busi (a2) and S. D'Amelio (a2)


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