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Molecular identification of Ancylostoma species from dogs and an assessment of zoonotic risk in low-income households, São Paulo State, Brazil

  • A.P. Oliveira-Arbex (a1), E.B. David (a1), T.C.G. Oliveira-Sequeira (a1), S. Katagiri (a2), S.T. Coradi (a3) and S. Guimarães (a1)...

Abstract

Hookworm infection stands out for its worldwide distribution and for its veterinary and public health relevance. Based on copromicroscopic examinations and polymerase chain reaction (PCR) amplification of the ITS1–5.8S–ITS2 region, we assessed, respectively, the prevalence of intestinal parasites and the identification of canine hookworm species in faeces recovered from 278 dogs living in households of an inland municipality of São Paulo State, Brazil. Intestinal parasites were found in 67.3% of dogs and hookworm infection was found at the highest prevalence rate (56.6%), followed by Toxocara canis (11.9%), Isospora spp. (11.9%), Giardia spp. (5.8%), Sarcocystis spp. (4.0%), ‘Hammondia-like’ (1.4%), Dipylidium caninum (1.1%) and Trichuris vulpis (0.7%). Of 158 samples positive for hookworm eggs, 106 (67.1%) were amplified by PCR and, of those, 88 (55.7%) were successfully sequenced for species identification. Single infections with Ancylostoma caninum and Ancylostoma braziliense were recorded in 61.4% and 12.5%, respectively, and mixed infections were found in 26.1%. The nucleotide sequences of both species showed high identity rates (98–100%) when compared with reference sequences. Although A. caninum was the most prevalent hookworm in the dogs assessed, the occurrence of both A. caninum and A. braziliense in single and/or mixed infections poses a potential risk for the local population in a low-income area, especially children, to acquire cutaneous larva migrans (CLM).

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Bowman, D.D., Montgomery, S.P., Zajac, A.M., Eberhard, M.L. & Kazacos, K.R. (2010) Hookworms of dogs and cats as agents of cutaneous larva migrans. Trends in Parasitology 26, 6267.
Cardoso, A.S., Costa, I.M.H., Figueiredo, C., Castro, A. & Conceição, M.A.P. (2014) The occurrence of zoonotic parasites in rural dog populations from northern Portugal. Journal of Helminthology 88, 203209.
Coelho, W.M.D., Amarante, A.F.T., Apolinário, J.D.C., Coelho, N.M.D. & Bresciani, K.D.S. (2011) Occurrence Ancylostoma in dogs, cats and public places from Andradina city, São Paulo state, Brazil. Revista do Instituto de Medicina Tropical de Sao Paulo 53, 181184.
David, E.B., Coradi, S.T., Oliveira-Sequeira, T.C.G., Ribolla, P.E.M., Katagiri, S. & Guimarães, S. (2011) Diagnosis of Giardia infections by PCR-based methods in children of an endemic area. Journal of Venomous Animals and Toxins including Tropical Diseases 17, 209215.
Fontanarrosa, M.F., Vezzani, D., Basabe, J. & Eiras, D.F. (2006) An epidemiological study of gastrointestinal parasites of dogs from Southern Greater Buenos Aires (Argentina): age, gender, breed, mixed infections, and seasonal and spatial patterns. Veterinary Parasitology 136, 283295.
Garcia, L.S. (2001) Diagnostic medical parasitology. 4th edn. 1092 pp. Washington, DC, ASM Press.
Gingrich, E.N., Scorza, A.V., Clifford, E.L., Olea-Popelka, F.J. & Lappin, M.R. (2010) Intestinal parasites of dogs on the Galapagos Islands. Veterinary Parasitology 169, 404407.
Heukelbach, J. & Feldmeier, H. (2008) Epidemiological and clinical characteristics of hookworm related cutaneous larva migrans. Lancet Infectious Diseases 8, 302309.
Heukelbach, J., Frank, R., Ariza, L., Lopes, I.S., Silva, A.D.A., Borges, A.C., Limongi, J.E., De Alencar, C.H.M. & Klimpel, S. (2012) High prevalence of intestinal infections and ectoparasites in dogs, Minas Gerais State (southeast Brazil). Parasitology Research 111, 19131921.
Katagiri, S. & Oliveira-Sequeira, T.C.G. (2008) Prevalence of dog intestinal parasites and risk perception of zoonotic infection by dog owners in Sao Paulo State, Brazil. Zoonoses Public Health 55, 406413.
Klimpel, S., Heukelbach, J., Pothmann, D. & Rückert, S. (2010) Gastrointestinal and ectoparasites from urban stray dogs in Fortaleza (Brazil): high infection risk for humans? Parasitology Research 107, 713719.
Larkin, M.A., Blackshields, G., Brown, N.P., Chenna, R., McGettigan, P.A., McWilliam, H., Valentin, F., Wallace, I.M., Wilm, A., Lopez, R., Thompson, J.D., Gibson, T.J., & Higgins, D.G. (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23, 29472948.
La Sala, L.F., Leiboff, A., Burgos, J.M. & Costamagna, S.R. (2015) Spatial distribution of canine zoonotic enteroparasites in Bahía Blanca, Argentina. Revista Argentina de Microbiologia 47, 1724.
Lesshafft, H., Schuster, A., Reichert, F., Talhari, S., Ignatius, R. & Feldmeier, H. (2012) Knowledge, attitudes, perceptions, and practices regarding cutaneous larva migrans in deprived communities in Manaus, Brazil. Journal of Infection in Developing Countries 6, 422429.
Liotta, J.L., Koompapong, K.N., Yaros, J.P., Prullage, J. & Bowman, D.D. (2012) Prevalence of Ancylostoma braziliense in cats in three northern counties of Florida, United States. Journal of Parasitology 98, 10321033.
Little, S.E., Johnson, E.M., Lewis, D., Jaklitsch, R.P., Payton, M.E., Blagburn, B.L., Bowman, D.D., Moroff, S., Tams, T., Rich, L. & Aucoin, D. (2009) Prevalence of intestinal parasites in pet dogs in the United States. Veterinary Parasitology 166, 4452.
Mahdy, M.A., Lim, Y.A., Ngui, R., Fatimah, M., Choy, S.H., Yap, N.J., Al-Mekhlafi, H.M., Ibrahim, J. & Surin, J. (2012) Prevalence and zoonotic potential of canine hookworms in Malaysia. Parasites & Vectors 5, 88.
Mandarino-Pereira, A., Souza, F.S., Lopes, C.W.G. & Pereira, M.J.S. (2010) Prevalence of parasites in soil and dog feces according to diagnostic tests. Veterinary Parasitology 170, 176181.
Marques, J.P., Guimarães, C.D.R., Boas, A.V., Carnaúba, P.U. & Moraes, J. (2012) Contamination of public parks and squares from Guarulhos (São Paulo State, Brazil) by Toxocara spp. and Ancylostoma spp. Revista do Instituto de Medicina Tropical de Sao Paulo 54, 267271.
Ngui, R., Lim, Y.A., Traub, R., Mahmud, R. & Mistam, M.S. (2012) Epidemiological and genetic data supporting the transmission of Ancylostoma ceylanicum among human and domestic animals. PLoS Neglected Tropical Diseases 6, 17.
Palmer, C.S., Traub, R.J., Robertson, I.D., Hobbs, R.P., Elliot, A., While, L., Rees, R. & Thompson, R.C.A. (2007) The veterinary and public health significance of hookworm in dogs and cats in Australia and the status of A. ceylanicum . Veterinary Parasitology 145, 304313.
Riggio, F., Mannella, R., Ariti, G. & Perrucci, S. (2013) Intestinal and lung parasites in owned dogs and cats from central Italy. Veterinary Parasitology 193, 7884.
Scorza, A.V., Duncan, C., Miles, L. & Lappin, M.R. (2011) Prevalence of selected zoonotic and vector borne agents in dogs and cats in Costa Rica. Veterinary Parasitology 183, 178183.
Soriano, S.V., Pierangeli, N.B., Roccia, I., Bergagna, H.F.J., Lazzarini, L.E., Celescinco, A., Saiz, M.S., Kossman, A., Contreras, A.P., Arias, C. & Basualdo, J.A. (2010) A wide diversity of zoonotic intestinal parasites infects urban and rural dogs in Neuquén, Patagonia, Argentina. Veterinary Parasitology 167, 8185.
Sprenger, L.K., Green, K.T. & Molento, M.B. (2014) Geohelminth contamination of public areas and epidemiological risk factors in Curitiba, Brazil. Revista Brasileira de Parasitologia Veterinária 23, 6973.
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. & Kumar, S. (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution 28 27312739.
Traub, R.J., Robertson, I.D., Irwin, P., Mencke, N. & Thompson, R.C.A. (2004a) Application of a species specific PCR-RFLP to identify Ancylostoma eggs directly from canine faeces. Veterinary Parasitology 123, 245255.
Traub, R.J., Robertson, I.D., Irwin, P., Mencke, N. & Thompson, R.C.A. (2004b) The prevalence, intensities and risk factors associated with geohelminth infection in tea-growing communities of Assam, India. Tropical Medicine and International Health 9, 688701.
Traub, R.J., Robertson, I.D., Irwin, P.J., Mencke, N. & Thompson, R.C.A. (2005) Canine gastrointestinal parasitic zoonoses in India. Trends in Parasitology 21, 4248.
Traub, R.J., Inpankaew, T., Sutthikornchai, C., Sukthana, Y. & Thompson, R.C.A. (2008) PCR-based coprodiagnostic tools reveal dogs as reservoirs of zoonotic ancylostomiasis caused by Ancylostoma ceylanicum in temple communities in Bangkok. Veterinary Parasitology 155, 6773.
Traversa, D., Frangipane di Regalbono, A., Di Cesare, A., La Torre, F., Drake, J. & Pietrobelli, M. (2014) Environmental contamination by canine geohelminths. Parasites & Vectors 7, 67.

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