Skip to main content Accessibility help

Molecular identification and seasonal infections of species of Fasciola in ruminants from two provinces in China

  • W. Yuan (a1), J.-M. Liu (a1), K. Lu (a1), H. Li (a1), M.-M. Duan (a1), J.-T. Feng (a1), Y. Hong (a1), Y.-P. Liu (a2), Y. Zhou (a3), L.-B. Tong (a4), J. Lu (a5), C.-G. Zhu (a1), Y.-M. Jin (a1), G.-F. Cheng (a1) and J.-J. Lin (a1) (a6)...


We determined the prevalence and seasonality of infections by Fasciola of goats and bovine species (cattle and water buffalo) in Hubei and Anhui provinces of China. Faecal samples were collected at 2- to 3-month intervals from 200 goats in Hubei province and from 152 bovine species in Anhui province. All faecal samples were examined for the presence of parasites. We determined the nucleotide sequences of the first and second internal transcribed spacers (ITS-1 and ITS-2) of the nuclear ribosomal DNA (rDNA) of 39 Fasciola worms from Anhui province. The prevalence of Fasciola infection in goats ranged between 3.5 and 37.0%, with mean eggs per gram (EPG) ranging between 29.0 and 166.0. Prevalence and EPG exhibited downward trends over time with significant differences. The prevalence of Fasciola infection in cattle ranged between 13.3 and 46.2% (mean EPG, 36.4–100.0), and that of water buffalo ranged between 10.3 and 35.4% (mean EPG, 25.0–89.6), with a higher prevalence of infection and EPG from June to October compared with December to March. Analysis of ITS-1 and ITS-2 sequences revealed that F. hepatica and F. gigantica were present in all bovine species of Anhui province and that F. gigantica mainly infected water buffalo. This is the first demonstration of Fasciola infection in Hubei province and detection of F. hepatica and F. gigantica in Anhui province. The present study of Hubei province shows that mass treatment of livestock with closantel sodium injections in April and August/September controlled Fasciola infection effectively.


Corresponding author

*Fax: +86 21 34293619 E-mail:


Hide All
Alasaad, S., Granados, J.E., Cano-Manuel, F.J., Meana, A., Zhu, X.Q. & Pérez, J.M. (2008) Epidemiology of fasciolosis affecting Iberian ibex (Capra pyrenaica) in southern Spain. Parasitology Research 102, 751755.
Ali, H., Ai, L., Song, H.Q., Ali, S., Lin, R.Q., Seyni, B., Issa, G. & Zhu, X.Q. (2008) Genetic characterization of Fasciola samples from different host species and geographical localities revealed the existence of F. hepatica and F. gigantica in Niger. Parasitology Research 102, 10211024.
Chen, J.X., Chen, M.X., Ai, L., Xu, X.N., Jiao, J.M., Zhu, T.J., Su, H.Y., Zhang, W., Luo, J.J., Guo, Y.H., Lv, S. & Zhou, X.N. (2013) An outbreak of human fascioliasis gigantica in southwest China. PLoS One 8, e71520.
Chen, S.B., Hasi, S.R. & Amu, G.L. (2013) Comparative analysis and digital description of the microscopic characteristics of nine kinds of helminth eggs of ruminants. Acta Zoologica Sinica 44, 931936 (in Chinese).
Dar, Y., Amer, S., Mercier, A., Courtioux, B. & Dreyfuss, G. (2012) Molecular identification of Fasciola sp. (Digenea: Fasciolidae) in Egypt. Parasite 19, 177182.
Dorny, P., Stoliaroff, V., Charlier, J., Meas, S., Sorn, S., Chea, B., Holl, D., Van Aken, D. & Vercuysse, J. (2011) Infection with gastrointestinal nematodes Fasciola and Paramphistomum in cattle in Cambodia and their association with mobidity parameters. Veterinary Parasitology 175, 293299.
Gu, Y., Shen, Y., Ferre, I., Kraemer, F. & Gonzaloz, J. (1998) Investigation and analysis of Fasciola hepatica infection of buffalo in Jiangsu and Anhui Provinces. Chinese Journal for Veterinary Science and Technology 28, 1416 (in Chinese).
Huang, D.M., Lin, X.M., Zhou, K.F., Chen, Q.Q. & Tang, C.T. (1997) Faecal examination of Fasciola spp. of bovines in Jiumu Town, Fujian Province. Journal of Tsinghua University (Science and Technology) 37, 4749 (in Chinese).
Itagaki, T., Kikawa, M., Sakaguchi, K., Shimo, J., Terasaki, K., Shibahara, T. & Fukuda, K. (2005a) Genetic characterization of parthenogenic Fasciola sp. in Japan on the basis of the sequences of ribosomal and mitochondrial DNA. Parasitology 131, 679685.
Itagaki, T., Kikawa, M., Terasaki, K., Shibahara, T. & Fukuda, K. (2005b) Molecular characterization of parthenogenic Fasciola sp. in Korea on the basis of DNA sequences of ribosomal ITS1 and mitochondrial NDI gene. Journal of Veterinary Medical Science 67, 11151118.
Khallaayoune, K.I., Stromberg, B.E., Dakkak, A. & Malone, J.B. (1991) Seasonal dynamics of Fasciola hepatica burdens in grazing Timahdit sheep in Morocco. International Journal for Parasitology 21, 307314.
Liao, D.J. (1996) Study on the new egg count technique for M. hirudinaceus and A. suum . Veterinary Parasitology 61, 113117.
Lin, R.Q., Dong, S.J. & Xue, C.X. (2004) DNA polymorphism of the first internal transcribed spacer of Fasciola spp. Chinese Journal for Veterinary Science and Technology 34, 812 (in Chinese).
Lin, R.Q., Dong, S.J., Nie, K., Wang, C.R., Li, A.X., Song, H.Q., Huang, W.Y. & Zhu, X.Q. (2007) Sequence analysis of the first internal transcribed spacer of rDNA supports the existence of an intermediate Fasciola between F. hepatica and F. gigantica in Mainland China. Parasitology Research 101, 813817.
Lin, Y.G., Sun, Y.L., Chen, C.R. & Chen, D.X. (1974) The developmental history of Fasciola gigantica and epidemiology study of Fasciola spp. Acta Zoologica Sinica 20, 378390 (in Chinese).
Luton, K., Waller, D. & Blair, D. (1992) Comparison of ribosomal internal transcribed spacers from two congeneric species of flukes (Platyhelminthes: Trematoda: Digenea). Molecular and Biochemical Parasitology 56, 323327.
Nginyi, J.M., Duncan, J.L., Mellor, D.J., Stear, L.J., Wanyangu, S.W., Bain, R.K. & Gatongi, P.M. (2001) Epidemiology of parasitic gastrointestinal nematode infections of ruminants on smallholder farms in central Kenya. Research in Veterinary Science 70, 3339.
Spithill, T.W. & Dalton, J.P. (1998) Progress in development of liver fluke vaccines. Parasitology Today 14, 224228.
Wang, D.Y., Zhang, W.Y. & Huang, W.Y. (2006) Investigation on the infection of Fasciola spp. of bovines in Guangxi Province. Veterinary Animal Husbandry in Guangxi 22, 200202 (in Chinese).
Xie, Y.J., Liu, W., Yang, J.H., Li, X.H. & Tan, X.Q. (2005) Investigation on the infection of Fasciola hepatica of bovines in Yueyang Hunan Province. Chinese Journal of Veterinary Parasitology 13, 2021 (in Chinese).
Xu, L.Q., Yu, S.H. & Xu, S.M. (1999) Distribution and pathogenic impact of human parasites in China. 928 pp. Beijing, Beijing People's Medical Publishing House.
Zumaquero-Ríos, J.L., Sarracent-Pérez, J., Rojas-García, R., Rojas-Rivero, L., Martínez-Tovilla, Y., Valero, M.A. & Mas-coma, S. (2013) Fascioliasis and intestinal parasitoses affecting school children in Atlixco, Puebla State, Mexico: epidemiology and treatment with nitazoxanide. PLoS Neglected Tropical Diseases 7, e2553.


Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed