Skip to main content Accessibility help
×
Home

Molecular analysis of human- and pig-derived Ascaris in Honduras

  • A. Palma (a1), B. Ortiz (a1), L. Mendoza (a1), G. Matamoros (a2), J.A. Gabrie (a3), A.L. Sánchez (a3) and G. Fontecha (a4)...

Abstract

Ascaris sp. is a soil-transmitted helminth (STH) significantly affecting the health of human and swine populations. Health inequities and poverty, with resulting deficiencies in water, sanitation and hygiene, are directly associated with Ascaris lumbricoides prevalence in humans. Resource constraints also lead to small-scale livestock production under unsanitary conditions. Free-ranging pigs, for instance, are exposed to a number of infectious agents, among which Ascaris suum is one of the most common. Under these conditions, close proximity between people and pigs can result in cross-contamination; that is, pigs harbouring human Ascaris and vice versa. Moreover, the potential interbreeding between these two Ascaris species has been demonstrated. The present study analysed Ascaris worms obtained from children and pigs in Honduras. Adult worms were collected from stool samples of children after pharmacological treatment, and from pigs’ intestines after slaughter for commercial purposes at a local abattoir. A nuclear ribosomal internal transcribed spacer (ITS) region was amplified by polymerase chain reaction (PCR) and digested with a restriction enzyme in order to separate putative human- and pig-derived Ascaris isolates. PCR products were also sequenced, and cladograms were constructed. All parasites isolated from children showed the typical human-derived genotype of Ascaris, whereas 91% of parasites from pigs showed the expected pig-derived genotype. Cross-infections between hosts were not demonstrated in this study. Nine per cent of pig-derived worms showed a restriction band pattern highly suggestive of a hybrid human–pig Ascaris genotype. These results contribute to the understanding of ascariasis epidemiology and its zoonotic potential in a highly endemic region.

Copyright

Corresponding author

Author for correspondence: G. Fontecha, E-mail: gustavo.fontecha@unah.edu.hn

References

Hide All
Anderson, TJ (1995) Ascaris infections in humans from North America: molecular evidence for cross-infection. Parasitology 110, 215219.
Anderson, TJ and Jaenike, J (1997) Host specificity, evolutionary relationships and macrogeographic differentiation among Ascaris populations from humans and pigs. Parasitology 115, 325342.
Anderson, TJ, Romero-Abal, ME and Jaenike, J (1993) Genetic structure and epidemiology of Ascaris populations: patterns of host affiliation in Guatemala. Parasitology 107, 319334.
Arizono, N, Yoshimura, Y, Tohzaka, N, Yamada, M, Tegoshi, T, Onishi, K and Uchikawa, R (2010) Ascariasis in Japan: is pig-derived Ascaris infecting humans? Japanese Jornal of Infectious Diseases 63, 447448.
Bendall, RP, Barlow, M, Betson, M, Stothard, JR and Nejsum, P (2011) Zoonotic ascariasis, United Kingdom. Emerging Infectious Diseases 17, 19641966.
Betson, M, Nejsum, P, Bendall, RP, Deb, RM and Stothard, JR (2014) Molecular epidemiology of ascariasis: a global perspective on the transmission dynamics of Ascaris in people and pigs. Journal of Infectious Diseases 210, 932941.
Brooker, S, Clements, AC and Bundy, DA (2006) Global epidemiology, ecology and control of soil-transmitted helminth infections. Advances in Parasitology 62, 221261.
Carlsgart, J, Roepstorff, A and Nejsum, P (2009) Multiplex PCR on single unembryonated Ascaris (roundworm) eggs. Parasitology Research 104, 939943.
Crewe, W and Smith, DH (1971) Human infection with pig Ascaris. (A. suum). Annals of Tropical Medicine and Parasitology 65, 85.
Criscione, CD, Anderson, JD, Raby, K, et al. (2007a) Microsatellite markers for the human nematode parasite Ascaris lumbricoides: development and assessment of utility. Journal of Parasitology 93, 704708.
Criscione, CD, Anderson, JD, Sudimack, D, Peng, W, Jha, B, Williams-Blangero, S and Anderson, TJ (2007b) Disentangling hybridization and host colonization in parasitic roundworms of humans and pigs. Proceedings. Biological Sciences 274, 26692677.
Dutto, M and Petrosillo, N (2013) Hybrid Ascaris suum/lumbricoides (Ascarididae) infestation in a pig farmer: a rare case of zoonotic ascariasis. Central European Journal of Public Health 21, 224226.
Hotez, PJ, Bottazzi, ME, Franco-Paredes, C, Ault, SK and Periago, MR (2008) The neglected tropical diseases of Latin America and the Caribbean: a review of disease burden and distribution and a roadmap for control and elimination. PLoS Neglected Tropical Diseases 2, e300.
INE (Instituto Nacional de Estadística de Honduras) (2016) Hogares en condición de pobreza. Secondary Hogares en condición de pobreza. Available at http://www.ine.gob.hn/images/Productos%20ine/Boletines/Boletin%202017/BOLETIN%20CIFRAS%20DE%20PAIS%202010%20-%202016.pdf (accessed 4 December 2017).
Iniguez, AM, Leles, D, Jaeger, LH, Carvalho-Costa, FA, Araujo, A; Amazonas Research Group (2012) Genetic characterisation and molecular epidemiology of Ascaris spp. from humans and pigs in Brazil. Transactions of the Royal Society of Tropical Medicine and Hygiene 106, 604612.
Jungersen, G, Eriksen, L, Nielsen, CG, Roepstorff, A and Nansen, P (1996) Experimental transfer of Ascaris suum from donor pigs to helminth naive pigs. Journal of Parasitology 82, 752756.
Leles, D, Araujo, A, Vicente, AC and Iniguez, AM (2010) ITS1 intra-individual variability of Ascaris isolates from Brazil. Parasitology International 59, 9396.
Leles, D, Gardner, SL, Reinhard, K, Iniguez, A and Araujo, A (2012) Are Ascaris lumbricoides and Ascaris suum a single species? Parasites & Vectors 5, 42.
Macko, JK and Dubinsky, P (1997) Taxonomic deliberations on human and pigs ascarids. Helminthologia 34, 167171.
Mejia Torres, RE, Franco Garcia, DN, Fontecha Sandoval, GA, Hernandez Santana, A, Singh, P, Mancero Bucheli, ST, Saboya, M and Paz, MY (2014) Prevalence and intensity of soil-transmitted helminthiasis, prevalence of malaria and nutritional status of school going children in Honduras. PLoS Neglected Tropical Diseases 8, e3248.
Miller, LA, Colby, K, Manning, SE, et al. (2015) Ascariasis in humans and pigs on small-scale farms, Maine, USA, 2010–2013. Emerging Infectious Diseases 21, 332334.
Nadler, SA and Hudspeth, DS (2000) Phylogeny of the Ascaridoidea (Nematoda: Ascaridida) based on three genes and morphology: hypotheses of structural and sequence evolution. Journal of Parasitology 86, 380393.
Nejsum, P, Parker, ED Jr, Frydenberg, J, Roepstorff, A, Boes, J, Haque, R, Astrup, I, Prag, J and Skov Sorensen, UB (2005) Ascariasis is a zoonosis in Denmark. Journal of Clinical Microbiology 43, 11421148.
Nejsum, P, Betson, M, Bendall, RP, Thamsborg, SM and Stothard, JR (2012) Assessing the zoonotic potential of Ascaris suum and Trichuris suis: looking to the future from an analysis of the past. Journal of Helminthology 86, 148155.
Nejsum, P, Hawash, MB, Betson, M, Stothard, JR, Gasser, RB and Andersen, LO (2017) Ascaris phylogeny based on multiple whole mtDNA genomes. Infection, Genetics and Evolution 48, 49.
Peng, W and Criscione, CD (2012) Ascariasis in people and pigs: new inferences from DNA analysis of worm populations. Infections, Genetics and Evolution 12, 227235.
Peng, W, Yuan, K, Zhou, X, Hu, M, Abs El-Osta, YG and Gasser, RB (2003) Molecular epidemiological investigation of Ascaris genotypes in China based on single-strand conformation polymorphism analysis of ribosomal DNA. Electrophoresis 24, 23082315.
Peng, W, Yuan, K, Hu, M, Zhou, X and Gasser, RB (2005) Mutation scanning-coupled analysis of haplotypic variability in mitochondrial DNA regions reveals low gene flow between human and porcine Ascaris in endemic regions of China. Electrophoresis 26, 43174326.
Peng, W, Yuan, K, Hu, M and Gasser, RB (2007) Recent insights into the epidemiology and genetics of Ascaris in China using molecular tools. Parasitology 134, 325330.
Sanchez, AL, Gabrie, JA, Usuanlele, MT, Rueda, MM, Canales, M and Gyorkos, TW (2013) Soil-transmitted helminth infections and nutritional status in school-age children from rural communities in Honduras. PLoS Neglected Tropical Diseases 7, e2378.
Sanchez, AL, Gabrie, JA, Rueda, MM, Mejia, RE, Bottazzi, ME and Canales, M (2014) A scoping review and prevalence analysis of soil-transmitted helminth infections in Honduras. PLoS Neglected Tropical Diseases 8, e2653.
Sanchez, AL, Gabrie, JA, Canales, M, Rueda, MM, Fontecha, GA, Mason, P, Bearmen, G and Steven, MP (2016) Soil-transmitted helminths, poverty, and malnutrition in Honduran children living in remote rural communities. Human Parasitic Diseases 8, 2735.
Sarti, E, Schantz, PM, Plancarte, A, Wilson, M, Gutierrez, IO, Lopez, AS, Roberts, J and Flisser, A (1992) Prevalence and risk factors for Taenia solium taeniasis and cysticercosis in humans and pigs in a village in Morelos, Mexico. American Journal of Tropical Medicine and Hygiene 46, 677685.
Shao, CC, Xu, MJ, Alasaad, S, Song, HQ, Peng, L, Tao, JP and Zhu, XQ (2014) Comparative analysis of microRNA profiles between adult Ascaris lumbricoides and Ascaris suum. BMC Veterinary Research 10, 99.
Soe, MJ, Kapel, CM and Nejsum, P (2016) Ascaris from humans and pigs appear to be reproductively isolated species. PLoS Neglected Tropical Diseases 10, e0004855.
Sparks, AM, Betson, M, Oviedo, G, Sandoval, C, Cooper, PJ and Stothard, JR (2015) Characterization of Ascaris from Ecuador and Zanzibar. Journal of Helminthology 89, 512515.
Takata, I (1951) Experimental infection of man with Ascaris of man and the pig. The Kitasato Archives of Experimental Medicine 23, 151159 (English transl., 49–59).
Umar, AS and Nura, A (2008) Training needs and risk assessment among farmers and animal handlers on zoonoses in Sokoto metropolis. The Nigerian Postgraduate Medical Journal 15, 168174.
Zhou, C, Li, M, Yuan, K, Deng, S and Peng, W (2012) Pig Ascaris: an important source of human ascariasis in China. Infection, Genetics and Evolution 12, 11721177.
Zhu, X, Chilton, NB, Jacobs, DE, Boes, J and Gasser, RB (1999) Characterisation of Ascaris from human and pig hosts by nuclear ribosomal DNA sequences. International Journal for Parasitology 29, 469478.

Molecular analysis of human- and pig-derived Ascaris in Honduras

  • A. Palma (a1), B. Ortiz (a1), L. Mendoza (a1), G. Matamoros (a2), J.A. Gabrie (a3), A.L. Sánchez (a3) and G. Fontecha (a4)...

Metrics

Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed