Hostname: page-component-78c5997874-t5tsf Total loading time: 0 Render date: 2024-11-18T18:52:18.810Z Has data issue: false hasContentIssue false
  • English
  • Français

Modulation of liver fibrosis and pathophysiological changes in mice infected with Mesocestoides corti (M. vogae) after administration of glucan and liposomized glucan in combination with vitamin C

Published online by Cambridge University Press:  12 April 2024

G. Ditteová ,
S. Velebný  and
G. Hrčkova
G. Ditteová
Affiliation:
Parasitological Institute, Slovak Academy of Sciences, Hlinkova 3, 040 01 Košice, Slovak Republic
S. Velebný
Affiliation:
Parasitological Institute, Slovak Academy of Sciences, Hlinkova 3, 040 01 Košice, Slovak Republic
G. Hrčkova*
Affiliation:
Parasitological Institute, Slovak Academy of Sciences, Hlinkova 3, 040 01 Košice, Slovak Republic
*
*Fax: 00421 55-6331414 E-mail: hrcka@saske.sk
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The effects of glucan and liposomized glucan, alone or co-administered with vitamin C, and empty liposomes on hepatic fibrosis in mice infected with Mesocestoides corti (M. vogae) tetrathyridia were studied. Preparations were administered every third day from day 7 to day 31 post-infection (p.i.), nine doses in total. Activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and cholesterol levels were measured in sera collected on days 11, 15, 21, 28, 32, 42, 50 and 65 p.i. Liver fibrosis was studied on the same days by measuring hydroxyproline concentration, which is considered a marker for collagen content. Larvicidal effects of the glucan and liposome preparations were estimated on day 65 p.i. in the liver and peritoneal cavity. Glucan formulations significantly enhanced collagen content, most prominently after administration of liposomized glucan in combination with vitamin C. Activities of both enzymes and cholesterol levels were slightly modified after administration of glucan alone. Liposomized glucan with vitamin C significantly increased ALT and AST activity and cholesterol levels up to days 28–32 p.i., after which they plateaued or declined. The most pronounced decrease was after administration of liposomized glucan and vitamin C. The same pattern of biochemical parameters in serum was observed after administration of empty liposomes, however, collagen content was not modified significantly. Larval counts in the liver and the peritoneal cavity were significantly reduced after treatment with either glucan formulation, but were unaffected following treatment with empty liposomes. In summary, intense fibrosis in the liver of mice treated with liposomized glucan and vitamin C did not result in the most extensive parenchymal cell injury but, rather in the highest efficacy of treatment. Liposomal lipids were probably utilized in the reparation of the damaged parenchymal cells, while glucan stimulated phagocytic cells.

Type
Review Article
Information
Journal of Helminthology , Volume 77 , Issue 3 , September 2003 , pp. 219 - 226
Copyright
Copyright © Cambridge University Press 2003

References

Alving, C.R. (1983) Delivery of liposome-encapsulated drugs to macrophages. Pharmacology and Therapeutics 22, 407–424.CrossRefGoogle ScholarPubMed
Aramaki, Y., Murai, M. & Tsuchiya, S. (1994) Activation of Fc receptor-mediated phagocytosis by mouse peritoneal macrophages following the intraperitoneal administration of liposomes. Pharmaceutical Research 11, 518–521.CrossRefGoogle ScholarPubMed
Bangham, D.A., Standish, M.M. & Watkins, J.C. (1965) Diffusion of univalent ions across the lamellae of swollen phospholipids. Journal of Molecular Biology 13, 238–252.CrossRefGoogle ScholarPubMed
Benková, M., Borošková, Z., Šoltýs, J., Dubaj, J. & Szechényi, Š. (1992) Effect of glucan preparation on immunocompetent cells and phagocytic ability of blood leucocytes in experimental ascariosis of pigs. Veterinary Parasitology 41, 157–166.CrossRefGoogle ScholarPubMed
Bresson-Hadni, S., Petitjean, O., Monnet-Jacquard, B., Heyd, B., Kantelip, B., Deschaseaux, M., Racadot, E. & Vuitton, D.A. (1994) Cellular localisations of interleukin–1beta, interleukin-6 and tumor necrosis factor-alpha mRNA in a parasitic granulomatous disease of the liver, alveolar echinococcosis. European Cytokine Network 5, 461–468.Google Scholar
Burt, A.D. & Oakley, C.L. (1993) Cellular and molecular aspects of hepatic fibrosis. Journal of Pathology 170, 105–114.CrossRefGoogle ScholarPubMed
Etges, F.J. (1991) The proliferative tetrathyridium of Mesocestoides vogae sp.n. (Cestoda). Proceedings of the Helminthological Society of Washington 58, 181–185.Google Scholar
Fidler, J.J. & Kleinerman, E.S. (1994) Clinical application of phospholipid liposomes containing macrophage activators for therapy of cancer metastasis. Advanced Drug Delivery Reviews 13, 325–340.CrossRefGoogle Scholar
Friedman, S.L. (1993) Seminars in medicine of the Beth Israel Hospital, Boston. The cellular basis of hepatic fibrosis. Mechanisms and treatment strategies. New England Journal of Medicine 328, 1828–1835.Google Scholar
Gallin, E.K., Green, S.W. & Patchen, M.L. (1992) Comparative effects of particulate and soluble glucan on macrophages of C3H/Hen and C3H/HeJ mice. International Journal for Immunopharmacology 14, 173–183.CrossRefGoogle ScholarPubMed
George, J. & Chandrakasan, G. (2000) Biochemical abnormalities during the progression of hepatic fibrosis induced by dimethylnitrosamine. Clinical Biochemistry 33, 563–570.CrossRefGoogle ScholarPubMed
Gregoriadis, G (1988) Liposomes as drug carriers. Recent trends and progress. Chichester, New York: John Wiley & Sons Ltd.Google Scholar
Guerret, S., Vuitton, D.A., Liance, M., Pater, C. & Carbillet, J.P. (1998) Echinococcus multilocularis: relationship and liver fibrogenesis in experimental mice. Parasitology Research 84, 657–667.CrossRefGoogle ScholarPubMed
Hoffmann, K.F., Caspar, P., Cheever, A.W. & Wynn, T. (1998) IFN-γ, IL-12, and TNF-α are required to maintain reduced liver pathology in mice vaccinated with Schistosoma mansoni eggs and IL-12. Journal of Immunology 161, 4201–4210.CrossRefGoogle ScholarPubMed
Hrčkova, G. & Velebný, S. (1995) Effects of free and liposomised praziquantel on worm burden and antibody response in mice infected with Mesocestoides corti tetrathyridia. Journal of Helminthology 69, 213–221.CrossRefGoogle Scholar
Hrčkova, G., Velebný, S. & Dezfuli, B.S. (1997) Albendazole treatment and liver fibrosis in mice infected with Mesocestoides vogae (syn M. corti) tetrathyridia (Cestoda): a preliminary study. Helminthologia 34, 197–205.Google Scholar
Jenkins, P., Dixon, J.B., Rakha, N.K. & Carter, S.D. (1990) Regulation of macrophage-mediated larvicidal activity in Echinococcus granulosus and Mesocestoides corti (Cestoda) infection in mice. Parasitology 100, 309–315.CrossRefGoogle ScholarPubMed
Jenkins, P., Spiers, S., Dixon, J.B., Carter, S.D. & May, S. (1992) The effects of tumour necrosis factor on host–parasite relations in murine Mesocestoides corti (Cestoda) infection. Parasitology 105, 453–459.CrossRefGoogle ScholarPubMed
Kadian, S.K., Dixon, J.B., Green, J.R., Carter, S.D. & Jenkins, P. (1994) Modification of macrophage–T cell interaction during infection of mice with Mesocestoides corti (Cestoda). Parasitology 109, 591–597.CrossRefGoogle ScholarPubMed
Kidd, P.M. (2000) The use of mushroom glucans and proteoglycans in cancer treatment. Alternative Medicine Review 5, 4–27.Google ScholarPubMed
Mallat, A., Preaux, A.-M., Blazejewski, S., Rosenbaum, J., Dhumeaux, D. & Mavier, P. (1995) Interferon alfa and gamma inhibit proliferation and collagen synthesis of human Ito cells in culture. Hepatology 21, 1003–1010.CrossRefGoogle ScholarPubMed
Mayell, M. (2001) Maitake extracts and their therapeutic potential. Alternative Medicine Review 6, 48–60.Google ScholarPubMed
Mizuno, T., Saito, H. & Nishitoba, T. (1995) Antitumor-active substances from mushrooms. Food Reviews International 11, 23–61.CrossRefGoogle Scholar
Murad, S., Sivarajah, A. & Pinnell, S.R. (1981) Regulation of prolyl and lysyl hydroxylase activities in cultured skin fibroblasts by ascorbic acid. Biochemical and Biophysical Research Communications 14, 101 Google Scholar
Musil, J. (1990) Bases of the biochemistry of the morbid processes (in Czech). Prague: Avicenum.Google Scholar
Pollacco, S., Nicholas, W.L., Mitchell, G.F. & Stewart, A.C. (1978) T-cell dependent collagenous encapsulating response in the mouse liver to Mesocestoides corti (Cestoda). International Journal for Parasitology 8, 457–462.CrossRefGoogle ScholarPubMed
Rasmussen, L.T. & Seljelid, R. (1991) Novel immunomodulators with pronounced in vivo effects caused by stimulation of cytokine release. Journal of Cellular Biochemistry 46, 60–68.CrossRefGoogle ScholarPubMed
Sakurai, T., Kaise, T., Yadomae, T. & Matsubara, C. (1997) Different role of serum components and cytokines on alveolar macrophage activation by soluble fungal (1→3)-beta-D-glucan. European Journal of Pharmacology 334, 255–263.CrossRefGoogle Scholar
Scherphof, G., Roerdink, F., Dijkstra, J., Ellens, H., deZanger, R. & Wisse, E. (1983) Uptake of liposomes by rat and mouse hepatocytes and Kupffer cells. Biology of the Cell and Biologie Cellulaire 47, 47–58.Google Scholar
Schuppan, D., Schmid, M., Somasudaram, R., Ackermann, R., Ruehl, M., Nakamura, T. & Riecken, E.-O. (1998) Collagens in the liver extracellular matrix bind hepatocyte growth factor. Gastroenterology 114, 139–152.CrossRefGoogle ScholarPubMed
Specht, D. & Voge, M. (1965) Asexual multiplication of Mesocestoides tetrathyridia in laboratory animals. Journal of Parasitology 51, 268–272.CrossRefGoogle ScholarPubMed
Specht, D. & Widmer, E.A. (1972) Response of mouse liver to infection with tetrathyridia of Mesocestoides (Cestoda). Journal of Parasitology 58, 431–437.CrossRefGoogle ScholarPubMed
Steadman, R., Petersen, M.M., Topley, N., Williams, D., Matthews, N., Spur, B. & Williams, J.D. (1990) Differential augmentation by recombinant human tumor necrosis factor-alpha of neutrophil responses to particulate zymosan and glucan. Journal of Immunology 144, 2712–2718.CrossRefGoogle ScholarPubMed
Straubinger, R.M., Hong, K., Friend, D.S. & Papahadjopoulos, D. (1983) Endocytosis of liposomes and intracellular fate of encapsulated molecules: encounter with a low pH compartment after internalisation in coated vesicles. Cell 32, 1069–1079.CrossRefGoogle Scholar
Šoltýs, J., Borošková, Z., Dubinský, P., Tomašovičová, O., Auer, H. & Aspöck, H. (1996) Effect of glucan immunomodulator on the immune response and larval burdens in mice with experimental toxocarosis. Applied Parasitology 37, 161–167.Google ScholarPubMed
Takeishi, T., Hirano, K., Kobayashi, T., Hasegawa, G., Hatakeyama, K. & Naito, M. (1999) The role of Kupffer cells in liver regeneration. Archives of Histology and Cytology 62, 413–422.CrossRefGoogle ScholarPubMed
Thornton, B.P., Větvička, V., Pitman, M., Goldman, R.C. & Ross, G.D. (1996) Analysis of the sugar specificity and molecular location of the binding lectin site of complement receptor type 3 (CD11b/CD18). Journal of Immunology 156, 1235–1246.CrossRefGoogle ScholarPubMed
Tonks, A., Cooper, R.A., Price, A.J., Molan, P.C. & Jones, K.P. (2001) Stimulation of TNF-alpha release in monocytes by honey. Cytokine 14, 240–242.CrossRefGoogle ScholarPubMed
Topley, N., Steadman, R., Petersen, M.M., Spur, B. & Williams, J.D. (1987) Phagocytosis, the respiratory burst and the 5-lipooxygenase pathway are independently regulated by human polymorphonuclear leukocytes. Advances in the Biosciences 66, 123–129.Google Scholar
Tsukamoto, H. (1999) Cytokine regulation of hepatic stellate cells in liver fibrosis. Alcoholism: Clinical and Experimental Research 23, 911–916.CrossRefGoogle ScholarPubMed
Velebný, S. & Hrčkova, G. (1995) The effect of free and liposome-entrapped praziquantel on serum activity of aminotransferases in mice infected with Mesocestoides corti tetrathyridia. Helminthologia 32, 193–199.Google Scholar
Velebný, S., Tomašovičová, O., Hrčkova, G. & Dubinský, P.(1997) Toxocara canis in mice: are liposomes and immunomodulator able to enhance the larvicidal effect of the anthelminthic?. Helminthologia 34, 147–153.Google Scholar
Wassef, N.M. & Alving, C.R. (1987) Complement-dependent phagocytosis of liposomes by macrophages. Methods in Enzymology 149, 124–134.CrossRefGoogle ScholarPubMed
White, T.R., Thompson, R.C.A., Penhale, W.J., Pass, D.A. & Mills, J.N. (1982) Pathophysiology of Mesocestoides corti infection in the mouse. Journal of Helminthology 56, 145–153.CrossRefGoogle ScholarPubMed
White, T.R., Thompson, R.C.A., Penhale, W.J. & Chihara, G. (1988) The effect of lentinan on the resistance of mice to Mesocestoides corti . Parasitology Research 74, 563–568.CrossRefGoogle ScholarPubMed
WHO (1984) Guidelines for surveillance, prevention and control of echinococcosis/hydatidosis. 2nd edn, Geneva: World Health Organization.Google Scholar
Williams, D.L., Mueller, A. & Browder, W. (1996) Glucan-based macrophage stimulators. Clinical Immunotherapy 5, 392–399.CrossRefGoogle Scholar
Woessner, J.F. (1961) The determination of hydroxyproline in tissue and protein samples containing small proportion of this imino acid. Archives of Biochemistry and Biophysics 93, 440–447.CrossRefGoogle ScholarPubMed
Zilva, J.F. & Pannall, P.R. (1984) Plasma enzymes in diagnosis. pp 366–385 in Zilva, J.F. & Pannall, P.R.. Clinical chemistry in diagnosis and treatment. London: Lloyd-Luke (medical books) Ltd.Google Scholar
Zimmermann, T., Franke, H., Peuker, M. & Dargel, R. (1992) Quantitative studies on fatty acid metabolism in isolated parenchymal cells from normal and cirrhotic livers in rats. Journal of Hepatology 15, 10–16.CrossRefGoogle ScholarPubMed