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Methods of preservation and flotation for the detection of nematode eggs and coccidian oocysts in faeces of the forest musk deer

Published online by Cambridge University Press:  12 November 2015

X.L. Hu
Affiliation:
Laboratory of Non-invasive Research Technology for Endangered Species, College of Nature Conservation, Beijing Forestry University, No. 35 Tsinghua East Road, Haidian District, Beijing100083, China
G. Liu
Affiliation:
Institute of Wetland Research, Chinese Academy of Forestry, Haidian District, Beijing100091, China
W.X. Wang
Affiliation:
Laboratory of Non-invasive Research Technology for Endangered Species, College of Nature Conservation, Beijing Forestry University, No. 35 Tsinghua East Road, Haidian District, Beijing100083, China
R. Zhou
Affiliation:
Laboratory of Non-invasive Research Technology for Endangered Species, College of Nature Conservation, Beijing Forestry University, No. 35 Tsinghua East Road, Haidian District, Beijing100083, China
S.Q. Liu
Affiliation:
Laboratory of Non-invasive Research Technology for Endangered Species, College of Nature Conservation, Beijing Forestry University, No. 35 Tsinghua East Road, Haidian District, Beijing100083, China
L.H. Li*
Affiliation:
Laboratory of Non-invasive Research Technology for Endangered Species, College of Nature Conservation, Beijing Forestry University, No. 35 Tsinghua East Road, Haidian District, Beijing100083, China State Forestry Planning and Design Institute of Forest Products Industry, State Forestry Administration, Chaoyang District, Beijing100013, China
D.F. Hu*
Affiliation:
Laboratory of Non-invasive Research Technology for Endangered Species, College of Nature Conservation, Beijing Forestry University, No. 35 Tsinghua East Road, Haidian District, Beijing100083, China

Abstract

Parasitic infections influence the health of captive forest musk deer (Moschus berezovskii) and affect population increases. Nevertheless, there are few quantitative studies regarding forest musk deer parasites, and there is no common preservation method or flotation solution used for detection of faecal parasites because of the biology of the worms and the host physiological state. The objective of this study was to evaluate preservation and flotation methods for the detection of nematode eggs and coccidian oocysts in faeces of the forest musk deer. The McMaster technique was used to count nematode eggs and coccidian oocysts in 33 samples of faeces. For the nematode eggs, the differences among flotation solutions were significant (P< 0.01), with sodium nitrate being the best flotation solution, and the combination of freezing and sodium nitrate resulted in the greatest number of eggs per gram (EPG = 209.4 ± 67.8). For the coccidian oocysts, the interaction between preservation method and flotation solution was significant (P< 0.01), and the combination of formalin and sodium chloride yielded the greatest number of oocysts per gram (OPG = 1010.7 ± 162.3). The forest musk deer had a high prevalence of parasitic infections, with the parasite load of coccidia (96.4%) significantly greater than that of nematodes (71.9%, P< 0.01). These results confirm that captive forest musk deer suffer from serious parasitic invasions and demonstrate that the novel method described here could be utilized for parasitological diagnosis, detection and prevention in species of Moschidae and Cervidae.

Type
Research Papers
Copyright
Copyright © Cambridge University Press 2015 

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References

Amila, A., Risalat, T., Aysajan, T., Rizihan, A., Zhou, C. & Mahmut, H. (2014) Intestinal parasites of Tianshan red deer in Nanshan Mountains areas in Xinjiang. Acta Theriologica Sinica 34, 8792 (in Chinese).Google Scholar
Bowman, D.D. (2014) Georgis’ parasitology for veterinarians. Philadelphia, Elsevier Health Sciences.Google Scholar
Comer, K., Coles, G.C. & Hillyer, M. (2001) A national survey for anthelmintic resistant nematodes in thoroughbreds. Eighteenth International Conference of the World Association for the Advancement of Veterinary Parasitology, Stresa, Italy, August, vol. 26.Google Scholar
Cringoli, G. (2006) FLOTAC, a novel apparatus for a multivalent faecal egg count technique. Parassitologia 48, 381384.Google Scholar
Cringoli, G., Rinaldi, L., Veneziano, V., Capelli, G. & Scala, A. (2004) The influence of floatation solution, sample dilution and the choice of McMaster slide area (volume) on the reliability of the McMaster technique in estimating the faecal egg counts of gastrointestinal strongyles and Dicrocoelium dendriticum in sheep. Veterinary Parasitology 123, 121131.CrossRefGoogle ScholarPubMed
Cringoli, G., Rinaldi, L., Maurelli, M.P. & Utzinger, J. (2010) FLOTAC: new multivalent techniques for qualitative and quantitative copromicroscopic diagnosis of parasites in animals and humans. Nature Protocols 5, 503515.CrossRefGoogle Scholar
Dytham, C. (2003) Scheirer–Ray–Hare test. Choosing and using statistics: A biologist's guide. pp. 145150. Chichester, UK, John Wiley and Sons.Google Scholar
Flohr, C., Tuyen, L.N., Lewis, S., Minh, T.T., Campbell, J., Britton, J., Williams, H., Hien, T.T., Farrar, J. & Quinnell, R.J. (2007) Low efficacy of mebendazole against hookworm in Vietnam: two randomized controlled trials. American Journal of Tropical Medicine and Hygiene 76, 732736.Google Scholar
Foreyt, W.J. (1986) Recovery of nematode eggs and larvae in deer: evaluation of fecal preservation methods. Journal of the American Veterinary Medical Association 189, 10651067.Google Scholar
Foreyt, W.J. (2001) Veterinary parasitology reference manual. Ames, Iowa State University Press.Google Scholar
García-López, H.L., Rodríguez-Tovar, L.E. & Medina-De la Garza, C.E. (1996) Identification of Cyclospora in poultry. Emerging Infectious Diseases 2, 356.CrossRefGoogle ScholarPubMed
Gawor, J. (2006) Studies on small strongyles (Cyathostominae) and their eradication in riding horses with special reference to drug resistance. Warszawa, Instytut Parazytologii, Polska Akademia Nauk.Google Scholar
Gordon, H. & Whitlock, H.V. (1939) Helminth diagnostic techniques. Journal of the Council for Scientific and Industrial Research in Australia 12, 5052.Google Scholar
Haug, A., Williams, R.B. & Larsen, S. (2006) Counting coccidial oocysts in chicken faeces: a comparative study of a standard McMaster technique and a new rapid method. Veterinary Parasitology 136, 233242.Google Scholar
He, L., Wang, W.X., Li, L.H., Liu, B.Q., Liu, G., Liu, S.Q., Qi, L. & Hu, D.F. (2014) Effects of crowding and sex on fecal cortisol levels of captive forest musk deer. Biological Research 1, 4748.Google Scholar
Hobbs, R.P., Twigg, L.E., Elliot, A.D. & Wheeler, A.G. (1999) Factors influencing the fecal egg and oocyst counts of parasites of wild European rabbits Oryctolagus cuniculus (L.) in Southern Western Australia. Journal of Parasitology 85, 796802.CrossRefGoogle ScholarPubMed
Jagła, E., Śpiewak, J., Zaleśny, G. & Popiołek, M. (2013) Effect of storage and preservation of horse faecal samples on the detectability and viability of Strongylid nematode eggs and larvae. Bulletin of the Veterinary Institute in Pulawy 57, 161165.Google Scholar
Lang, D.M. (2013) Non-invasive study of steroid hormone and immune globulin in captive musk deer. Beijing, Beijing Forestry University (in Chinese).Google Scholar
Liu, C.Y. (2008) A study on reasons of death and physiological features for breeding forest musk deer. Shanghai, East China Normal University (in Chinese).Google Scholar
Liu, Z.B., Cheng, J.G. & Dai, X.Y. (2002) Regional distribution and measures of coccidiosis of captive forest musk deer. China Herbivores 22, 46 (in Chinese).Google Scholar
Lu, X.H., Qiao, J.Y., Wu, X.M. & Su, L.N. (2009) A review of main effects of musk-deer diseases: purulent, respiratory system and parasitic diseases. Journal of Economic Animal 13, 104107 (in Chinese).Google Scholar
Lu, X.H., Qiao, J.Y., Wu, X.M., Wang, J., Hu, H., Pei, J.F., Zhang, H.F., Feng, T., Wang, Q.J., Kong, F. & Li, D.F. (2010) An investigation on GI parasites of alpine musk deer in Qinghai province. Chinese Journal of Veterinary Medicine 12, 5657 (in Chinese).Google Scholar
McKenna, P.B. (1998) The effect of previous cold storage on the subsequent recovery of infective third stage nematode larvae from sheep faeces. Veterinary Parasitology 80, 167172.Google Scholar
Nielsen, M.K., Vidyashankar, A.N., Andersen, U.V., DeLisi, K., Pilegaard, K. & Kaplan, R.M. (2010) Effects of fecal collection and storage factors on strongylid egg counts in horses. Veterinary Parasitology 167, 5561.Google Scholar
Oppliger, A., Clobert, J., Lecomte, J., Lorenzon, P., Boudjemadi, K. & John-Alder, H.B. (1998) Environmental stress increases the prevalence and intensity of blood parasite infection in the common lizard Lacerta vivipara . Ecology Letters 1, 129138.Google Scholar
Pereckienė, A., Kaziūnaitė, V., Vyšniauskas, A., Petkevičius, S., Malakauskas, A., Šarkūnas, M. & Taylor, M.A. (2007) A comparison of modifications of the McMaster method for the enumeration of Ascaris suum eggs in pig faecal samples. Veterinary Parasitology 149, 111116.Google Scholar
Ran, X.L. (2013) Stress response and intestinal parasites in Taihangshan macaques within tourism area, Jiyuan. Zhengzhou, Zhengzhou University (in Chinese).Google Scholar
Rinaldi, L., Coles, G.C., Maurelli, M.P., Musella, V. & Cringoli, G. (2011) Calibration and diagnostic accuracy of simple floatation, McMaster and FLOTAC for parasite egg counts in sheep. Veterinary Parasitology 177, 345352.Google Scholar
Roepstorff, A. & Nansen, P. (1998) Epidemiology, diagnosis and control of helminth parasites of swine. Vol. 3. Rome, FAO.Google Scholar
Rupasinghe, D. & Ogbourne, C.P. (1978) Laboratory studies on the effect of temperature on the development of the free-living stages of some strongylid nematodes of the horse. Parasitology Research 55, 249253.Google Scholar
Sha, G.R., Zhang, H.X., Cai, Y.H. & Wang, H.Y. (1994) The first detecting of Strongyloides papillosus in domestic Moschus berezovskii . Animal Husbandry and Veterinary Medicine 1, 1012 (in Chinese).Google Scholar
Sha, G.R., Zhang, H.X., Cai, Y.H., Wang, H.Y. & Liu, H.Y. (1995) A survey on parasites of domestic Moschus berezovskii in Sichuan province. Chinese Journal of Veterinary Parasitology 1 (in Chinese).Google Scholar
Sha, G.R., Yang, G.Y. & Cai, Y.H. (1998) Studies on epidemiology of Moniezia sichuanensis in domestic Moschus berezovskii . Journal of China Agricultural University S2, 146146 (in Chinese).Google Scholar
Vadlejch, J., Petrtýl, M., Lukešová, D., Čadková, Z., Kudrnáčová, M., Jankovská, I. & Langrová, I. (2013) The concentration McMaster technique is suitable for quantification of coccidia oocysts in bird droppings. Pakistan Veterinary Journal 33, 291295.Google Scholar
Ward, M.P., Lyndal-Murphy, M. & Baldock, F.C. (1997) Evaluation of a composite method for counting helminth eggs in cattle faeces. Veterinary Parasitology 73, 181187.CrossRefGoogle ScholarPubMed
Wetzel, R. (1951) Verbesserte McMaster-Kammer zum Auszählen von Wurmeiern. Tierärztl Umsch 6, 209210.Google Scholar
Wu, J., Jiang, X.L., Yu, J.F., Ma, F.H. & Wang, S.Z. (1983) A research on parasites of forest musk deer. Chinese Wildlife 3(013), 3538 (in Chinese).Google Scholar
Wunderlin, E., Wild, P. & Eckert, J. (1997) Comparative reproduction of Cryptosporidium baileyi in embryonated eggs and in chickens. Parasitology Research 83, 712715.Google Scholar
Yang, G.Y., Sha, G.R., Cai, Y.H. & Cheng, J.G. (2001) Pathogenic morphological observation of Moniezia sichuanensis in domestic Moschus berezovskii . Livestock and Poultry Industry 3, 49 (in Chinese).Google Scholar
Ying, Z.H. (1992) One case of parasite invasions of forest musk deer. Chinese Journal of Veterinary Science and Technology 4, 36 (in Chinese).Google Scholar
Zajicek, D. (1978) Comparison of efficiency of 2 quantitative ovoscopical methods. Veterinarni Medicina 23, 275282.Google Scholar
Zhu, C.S. (1991) A preliminary survey on parasites of forest musk deer in Zhenping farm of Shaanxi province. Journal of Animal Science and Veterinary Medicine 3(011), 2628 (in Chinese).Google Scholar