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Levels of ecdysteroid-like material in adults of Nippostrongylus brasiliensis during the intestinal phase

  • B. Nembo (a1), P. Duie (a2), M. Garcia (a2), P. Breton (a1), P. Gayral (a1), P. Porcheron (a2) and F. Goudey-Perriere (a1)...


Total ecdysteroid-like immunoreactive material was assayed and quantified in adults of the parasitic nematode N. brasiliensis during the intestinal phase in the rat in order to detect possible physiological fluctuations in titre. Worms of the same sex isolated from one rat were pooled in order to quantify ecdysteroids using enzyme immunoassay (EIA). The concentration of ecdysteroids fluctuated during adult life according to the sex and the age of the parasite. Important differences of levels of ecdysteroid-like compounds between the two sexes of parasites were noted particularly at 128 and 168 h post-infection. The peak at 128 h, present in female, but not in male worms, corresponds to the time of egg-laying. Following HPLC-EIA analysis, the presence of ecdysone, 20-hydroxyecdysone and apolar ecdysteroid-like material was ascertained during this peak. The physiological role and the possible origin of ecdysteroids in this nematode are discussed.


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Barker, G. C., Chitwood, D. J. & Rees, H. H. (1990a) Ecdysteroids in helminths and annelids. Invertebrate Reproduction and Development, 18, 111.
Barker, G. C., Mercer, J. G., Rees, H. H. & Howells, R. E. (1990b) Functional roles of ecdysteroids in the filarial nematodes Dirofilaria immitis and Brugia pahangi. Invertebrate Reproduction and Development, 18, 103104.
Barker, G. C., Mercer, J. G., Rees, H. H. & Howells, R. E. (1991) The effects of the ecdysteroids on the microfilarial production by Brugia pahangi and on the control of meiotic reinitiation in the oocytes of Dirofilaria immitis. Parasitology Research, 77, 6571.
Barreteau, H., Trouvin, J. H., Goudey-Perriere, F., Jacquot, C. & Gayral, p. (1991) Biogenic amines and GABA in the larval and adult forms of the nematode Nippostrongylus brasiliensis. Comparative Biochemistry and Physiology, 100C, 445449.
Baswaid, S. (1990) Les ecdystéroïdes ches les Mammifères—Recherche sur leur origine, leur nature et leur signification clinique à l'intérieur ou non du contexte parasitaire. Thèse d'université, Montpellier. pp. 84.
Bottjer, K. P. & Bone, L. W. (1985) Nippostrongylus brasiliensis: Feeding activity in the mouse. International Journal for Parasitology, 15, 914.
Bottjer, K. P., Whisenton, L. R. & Weinstein, P. P. (1984) Ecdysteroid-like substance in Nippostrongylus brasiliensis. Journal of Parasitology, 70, 986987.
Cleator, M., Delves, C. J., Howells, R. E. & Rees, H. H. (1987) Identity and tissue localization of free and conjugated ecdysteroids in adults of Dirofilaria immitis and Ascaris suum. Molecular Biochemistry and Parasitology, 25, 93105.
Delves, C. J., Rees, H. H. & Howells, R. E. (1989) Egg production in Brugia pahangi (Nematoda, Filarioidea). Parasitology, 98, 105113.
Fleming, M. W. (1985) Ascaris suum: role of ecdysteroids in moulting. Experimental Parasitology, 60, 207210.
Fleming, M. W. (1987) Ecdysteroids during embryonation of eggs of Ascaris suum. Comparative Biochemistry and Physiology, 87A, 803805.
Fleming, M. W. (1990) Occurrence and function of ecdysteroids in parasitic nematodes. Invertebrate Reproduction and Development, 18, 113.
Garcia, M. (1991) Recherches sur l'origine, métabolisme et fonction des ecdystéroïdes chez les lnvertébrés non-Arthropodes. Doctorat ès Sciences Université Pierre et Marie-Curie, Paris pp. 232.
Golvan, Y. V. (1990) Les Nouvelles Techniques en Parasitologie. (editor, Flammarion, ) Paris, 306 pp.
Goudey-Perriere, F., Fokam-Simo, B., Maccario, J., Perriere, C. & Gayral, P. (1992) Effects of ecdysteroids on reproductive physiology of Nippostrongylus brasiliensis (Nematoda) in vivo. Comparative Biochemistry and Physiology, 103C, 105109.
Karlson, P. (1980) Ecdysone in retrospect and prospect. In: Progress in Ecdysone Research (editor, Hoffman, J. A.), Developments in Endocrinology, Vol. 7, Elsevier/North Holland Biomedical: Amsterdam. pp. 111.
Kassai, T. (1982) Handbook of Nippostrongylus brasiliensis. Commonwealth Agricultural Bureaux, Budapest, 275 pp.
Koolman, J., Walter, J. & Zahner, H. (1984) Ecdysteroids in helminths. In Biosynthesis, Metabolism and Mode of Action of Invertebrate Hormones (editors, Hoffman, J. A. & Porchet, M.), pp. 323330, Springer-Verlag: Berlin, Heidelberg, New York, Tokyo.
Lafont, R. (1991) Reverse endocrinology, or ‘hormones’ seeking functions. Insect Biochemistry, 21, 697721.
Mercer, J. G., Munn, A. E. & Rees, H. H. (1988) Caenorhabditis elegans: Occurrence and metabolism of ecdysteroids in adults and dauer larvae. Comparative Biochemistry and Physiology, 90B, 261267.
Mendis, A. H. W., Rose, M. E. and Goodwin, T. W. (1983) Ecdysteroids of the nematode Dirofilaria immitis. Molecular and Biochemical Parasitology, 9, 209229.
O'hanlon, G. M., Howarth, O. W. & Rees, H. H. (1987) Identification of ecdysone-25-O-β-D-glucopyranoside as a new metabolite of ecdysone in the nematode Parascaris equorum. Biochemical Journal, 248, 305307.
O'hanlon, G. M., Cleator, M., Mercer, J. G., Howells, R. E. & Rees, H. H. (1991) Metabolism and fate of ecdysteroids in the nematodes Ascaris suum and Parascaris equorum. Molecular and Biochemical Parasitology, 47, 179188.
Porcheron, P., Moriniere, M., Grassi, J. & Pradelles, P. (1989) Development of an enzyme immunoassay for ecdysteroids using acetylcholinesterase as label. Insect Biochemistry, 19, 117122.
Rees, H. H. (1989) Pathways of biosynthesis of ecdysone. In: Ecdysone (editor, Koolman, K.), Thieme: Stuttgart, pp. 152160.
Rees, H. H. & Mendis, A. H. W. (1984) The occurrence and possible physiological significance of ecdysteroids during nematode and cestode development. In: Biosynthesis, Metabolism and Mode of Action of Invertebrate Hormones (editors Hoffmann, J. and Porchet, M.), Springer-Verlag. Berlin, pp. 338345.
Swanson, J. A., Falvo, R. & Bone, L. W. (1984) Nippostrongylus brasiliensis: Effects of testosterone on reproduction and establishment. International Journal for Parasitology, 14, 241247.
Willet, J. D. (1980) Control mechanisms in nematodes. In: Nematodes as Biological Models (editor, Zuckerman, B. M.), Academic Press: New York, pp. 197225.
Yokogawa, S. (1922) The development of Heligmosomum muris, a nematode from intestine of the wild rat. Parasitology, 14, 127166.


Levels of ecdysteroid-like material in adults of Nippostrongylus brasiliensis during the intestinal phase

  • B. Nembo (a1), P. Duie (a2), M. Garcia (a2), P. Breton (a1), P. Gayral (a1), P. Porcheron (a2) and F. Goudey-Perriere (a1)...


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