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Immune responses of the argasid tick Ornithodoros moubata moubata induced by infection with the filarial worm Acanthocheilonema viteae

  • D. Hutton (a1), A.P. Reid (a1) and S. Townson (a1)

Abstract

Investigations were undertaken to determine whether the tick Ornithodoros moubatamoubata mounted a detectable immune response to primary and secondary infections with Acanthocheilonema viteae. Uninfected control tick survival rate was 70%, but only 45% in the primary infection group. Post-secondary infection survival rate (82%) was comparable to controls, indicating that these selected ticks had some protective advantage. Mean A. viteae infective larvae recovery from ticks with secondary infections was 31.4% lower than expected, suggesting the development of immunity. SDS–PAGE of haemolymph for proteins induced post-primary infection yielded a stronger signal at 45 kDa than controls, which was further elevated post-secondary infection. Proteins at 48, 22 and 16 to 18 kDa were detected in haemolymph from infected ticks but not seen from controls. The direct effect of haemolymph on microfilarial viability was examined using a novel in vitro assay; in these preliminary trials no differences were observed in parasite viability when exposed to haemolymph from infected or uninfected groups of ticks.

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*Address for correspondence: Tropical Parasitic Diseases Unit, Northwick Park Institute for Medical Research, Northwick Park and St Mark's Hospitals, Watford Road, Harrow, Middlesex, HA1 3UJ, UK Fax: 020 8869 3270 E-mail: s.townson@ic.ac.uk

References

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Binnington, K.C. & Obenchain, F.D. (1982) Structure and function of the circulatory, nervous and neuroendocrine systems of ticks. pp. 351398 in Physiology of ticks. Pergamon Press.
Boman, H.G. & Hultmark, D. (1987) Cell-free immunity in insects. Annual Review of Microbiology 41, 103126.
Chalk, R., Townson, H., Natori, S., Desmond, H. & Ham, P.J. (1994) Purification of an insect defensin from the mosquito, Aedes aegypti. Insect Biochemistry and Molecular Biology 24, 403410.
Chalk, R., Townson, H. & Ham, P.J. (1995) Brugia pahangi: the effects of cecropins on microfilariae in vitro and in Aedes aegypti. Experimental Parasitology 80, 401406.
Croft, S.L., East, J.S. & Molyneux, D.H. (1982) Anti-trypanosomal factor in the haemolymph of Glossina. Acta Tropica 39, 293302.
Grubhoffer, L., Veres, J. & Dusábek, F. (1991) Lectins as the molecular factors of recognition and defence reaction of ticks. pp. 381388 in Modern acarology, Vol. 2. Prague, Academia, and The Hague, SPB Academic Publishing bv.
Ham, P.J., Zulu, M.B. & Zahedi, M. (1988) In vitro haemagglutination and attenuation of microfilarial motility by haemolymph from individual blackflies (Simulium ornatum) infected with Onchocerca lienalis. Medical and Veterinary Entomology 2, 718.
Ham, P.J. (1992) Immunity in haematophagous insect vectors of parasitic infection. pp. 101149 in Advances in disease vector research. New York, Springer-Verlag.
Ham, P.J., Albuquerque, C., Baxter, A.J., Chalk, R. & Hagen, H.E. (1994) Approaches to vector control: new and trusted. 1. Humoral immune responses in blackfly and mosquito vectors of filariae. Transactions of the Royal Society of Tropical Medicine and Hygiene 88, 132135.
Hoffmann, J.A. (1995) Innate immunity of insects. Current Opinion in Immunology 7, 410.
Lackie, A.M. (1988) Immune mechanisms in insects. Parasitology Today 4, 98105.
Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) SDS-polyacrylamide gel electrophoresis of proteins. pp. 18.4718.57 in Molecular cloning. A laboratory manual. Cold Spring Harbor Publications.
Schwan, E.V., Hutton, D., Shields, K.J.B. & Townson, S. (1991) Artificial feeding and successful reproduction in Ornithodoros moubata moubata (Murray, 1877) (Acarina: Argasidae). Experimental and Applied Acarology 13, 107115.
Singh, D.P., Chatterjee, R.K. & Sen, A.B. (1988) Studies on susceptibility of Ornithodoros moubata as a vector of Dipetalonema viteae. Journal of Communicable Diseases 20, 220225.
Townson, H. & Chaithong, U. (1991) Mosquito host influences on development of filariae. Annals of Tropical Medicine and Parasitology 85, 149163.
Townson, S., Tagboto, S.K., Castro, J., Lujan, A., Awadzi, K. & Titanji, V.P.K. (1994) Comparison of the sensitivity of different geographical races of Onchocerca volvulus microfilariae to ivermectin: studies in vitro. Transactions of the Royal Society of Tropical Medicine and Hygiene 88, 101106.
Veres, J. & Grubhoffer, L. (1990) Detection and partial characterisation of a new plasma lectin in the haemolymph of the tick Ornithodoros tartakovskyi. Microbios Letters 45, 6164.

Immune responses of the argasid tick Ornithodoros moubata moubata induced by infection with the filarial worm Acanthocheilonema viteae

  • D. Hutton (a1), A.P. Reid (a1) and S. Townson (a1)

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