Skip to main content Accessibility help
×
Home

Identities of two Paragonimus species from Sri Lanka inferred from molecular sequences

  • M. Iwagami (a1), R.P.V.J. Rajapakse (a2), W. Paranagama (a2) and T. Agatsuma (a1)

Abstract

Metacercariae of Paragonimus spp. were obtained from field-collected freshwater crabs in Sri Lanka. Genomic DNA was extracted from single metacercariae. Two gene regions (partial mitochondrial cytochrome c oxidase subunit 1 (CO1) and the second internal transcribed spacer of the nuclear ribosomal gene repeat (ITS2)) were amplified using the polymerase chain reaction. Two differing sequences were obtained for each of these gene regions. Phylogenetic analyses placed the type 1 sequences as sister to a clade containing P. westermani and P. siamensis whereas the type 2 sequences were close to published sequences of P. siamensis from Thailand. The possible taxonomic status of these two types are discussed. This is the first report of molecular data about Paragonimus from Sri Lanka.

Copyright

Corresponding author

*Author for correspondence Fax: +81 88 880 2535 E-mail: agatsuma@med.kochi-ms.ac.jp

References

Hide All
Blair, D., Agatsuma, T., Watanobe, T., Okamoto, M. & Ito, A. (1997) Geographical genetic structure within the human lung fluke, Paragonimus westermani, detected from DNA sequences. Parasitology 115, 411417.
Blair, D., Le, T.H., Després, L. & McManus, D.P. (1999) Mitochondrial genes of Schistosoma mansoni. Parasitology 119, 303313.
Blair, D., Wu, B., Chang, Z.S., Gong, X., Agatsuma, T., Zhang, Y.N., Chen, S.H., Lin, J.X., Chen, M.G., Waikagul, J., Guevara, A.G., Feng, Z. & Davis, G.M. (1999) A molecular perspective on the genera Paragonimus Braun, Euparagonimus Chen and Paragonimus Chen. Journal of Helminthology 73, 295299.
Blair, D., Xu, Z.B. & Agatsuma, T. (1999) Paragonimiasis and genus Paragonimus. Advances in Parasitology 42, 113222.
Blair, D., Davis, G.M. & Wu, B. (2001) Evolutionary relationships between trematodes and snails emphasizing schistosomes and paragonimids. Parasitology 123 (Suppl), S229S243.
Bowles, J., Hope, M., Tiu, W.U., Liu, X.S. & McManus, D.P. (1993) Nuclear and mitochondrial genetic markers highly conserved between Chinese and Philippine Schistosoma japonicum. Acta Tropica 55, 217229.
Bowles, J., Blair, D. & McManus, D.P. (1995) A molecular phylogeny of the human schistosomes. Molecular Phylogenetics and Evolution 4, 103109.
Dissanaike, A.S. & Paramananthan, D.C. (1962) Paragonimus infection in wild carnivores in Ceylon. Ceylon Journal of Medical Science (D) 11, 2945.
Iwagami, M., Ho, L.Y., Su, K., Lai, P.F., Fukushima, M., Nakano, M., Blair, D., Kawashima, K. & Agatsuma, T. (2000) Molecular phylogeographic studies on Paragonimus westermani in Asia. Journal of Helminthology 74, 315322.
Kannangara, D.W.W. (1969) Occurrence of the lung fluke Paragonimus macrorchis Chen, 1962 in Ceylon. Ceylon Journal of Medical Science 18, 3338.
Kannangara, D.W.W. & Karunaratne, G.M.S. (1969) Paragonimus siamensis – the fourth lung fluke reported from Ceylon. Ceylon Journal of Medical Science 18, 6165.
Kawashima, K., Sugiyama, H. & Punsin, K. (1989) Paragonimus infection in crabs in Thailand. pp. 7579. in Kawashima, K., Ed.) Paragonimus in Asia: biology, genetic variation and speciation (II). Fukuoka, Shunpposha Photographic Printing Co. Ltd.
Kumar, S., Tamura, K. & Nei, M. (1993) MEGA (Molecular Evolutionary Genetic Analysis) ver. 1.01. Pennsylvania State University.
Kumar, S., Tamura, K., Jakobsen, I.B. & Nei, M. (2001) MEGA (Molecular Evolutionary Genetic Analysis) ver. 2. Bioinformatics.
Miyazaki, I. & Wykoff, D.E. (1965) On a new lung fluke Paragonimus siamensis n. sp found in Thailand. Japanese Journal of Parasitology 14, 251257.
Yaemput, S., Dekumyoy, P. & Visiassuk, K. (1994) The natural first intermediate host of Paragonimus siamensis (Miyazaki and Wykoff, 1965) in Thailand. Southeast Asian Journal of Tropical Medicine and Public Health 25, 284290.

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed