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Identification and characterization of immunodominant linear epitopes on the antigenic region of a serine protease in newborn Trichinella larvae

Published online by Cambridge University Press:  20 May 2015

Y. Yang ,
I. Vallée ,
S.A. Lacour ,
P. Boireau ,
S.P. Cheng  and
M.Y. Liu
Y. Yang*
Affiliation:
Key Lab of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun130062, PR China JRU BIPAR, Animal Health Laboratory, ANSES, Maisons-Alfort, France State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, PR China
I. Vallée
Affiliation:
JRU BIPAR, Animal Health Laboratory, ANSES, Maisons-Alfort, France
S.A. Lacour
Affiliation:
JRU BIPAR, Animal Health Laboratory, ANSES, Maisons-Alfort, France
P. Boireau
Affiliation:
Key Lab of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun130062, PR China JRU BIPAR, Animal Health Laboratory, ANSES, Maisons-Alfort, France
S.P. Cheng*
Affiliation:
State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, PR China
M.Y. Liu*
Affiliation:
Key Lab of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun130062, PR China Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yang zhou, 225009, PR China
*
*E-mail: yongyang811128@163.com, liumy@jlu.edu.cn and tcscsp@126.com
*E-mail: yongyang811128@163.com, liumy@jlu.edu.cn and tcscsp@126.com
*E-mail: yongyang811128@163.com, liumy@jlu.edu.cn and tcscsp@126.com
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Abstract

An immunodominant serine protease of Trichinella spiralis named NBL1 showed encouraging potential in early diagnosis of trichinellosis in pigs and elicited protective immune responses during infection of animals. To further define serological reagents for diagnostic use, the specific epitopes on NBL protein recognized by the antibody responses of different susceptible hosts need to be defined. The present study described comprehensive mapping of immunodominant linear epitopes in the antigenic region (NBL-C, the C-terminal part of the protein) using various serum samples obtained from three kinds of hosts – pig, wild boar and mice. We identified six peptides which were commonly recognized by sera from pigs experimentally infected with Trichinella and pigs immunized with rNBL1-C; five and four peptides were recognized by sera from wild boars and mice infected with Trichinella, respectively. Three peptides (NBL1-6, -7 and -9) were commonly recognized by antisera in all three hosts, which share the sequence PSSGSRPTYP. We also found that one peptide (NBL1-12) was only recognized by antibodies from pigs immunized with rNBL1-C. The identification of specific epitopes targeted by the host antibody response is important both for understanding the natural response to infection and for the development of subunit vaccines and diagnostic tools for trichinellosis.

Type
Research Papers
Information
Journal of Helminthology , Volume 90 , Issue 2 , March 2016 , pp. 232 - 237
Copyright
Copyright © Cambridge University Press 2015 

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