Skip to main content Accessibility help
×
Home

Humoral immune responses induced by Gymnorhynchus gigas extracts in BALB/c mice

  • M. Rodero (a1) and C. Cuéllar (a1)

Abstract

The aim of this study was to determine if the plerocercoid larvae of Gymnorhynchus gigas, a common cestode of the ray’s bream (Brama raii), possess antigenic compounds potentially capable of provoking anaphylactic episodes. A murine experimental model, using BALB/c mice, was developed to study the humoral immune response induced by G. gigas extracts. A highly specific humoral immune response was detected and cross-reactions were not observed between parasite and host antigens. The presence of IgM and IgG3 levels suggest the presence of thymus-independent antigens in the parasitic extract. The IgG antibody class showed the highest levels, with the IgG1 the predominant subclass. These IgG1 levels are in accordance with the supposed presence of a type I allergic reaction after the ingestion of G. gigas plerocercoids parasitizing fish, as well as inducing anaphylaxia in fish. These results indicate that somatic products released from ingested larvae of G. gigas could induce the development of a Th2 response capable of causing allergic disorders.

Copyright

Corresponding author

*Author for correspondence: +Fax: 394 18 15 E-mail: cuellarh@eucmax.sim.ucm.es

References

Hide All
Audícana, M., Fernández, L., Muñoz, M., Fernández, E., Navarro, J. & Del Pozo, D. (1995) Recurrent anaphylaxis caused by Anisakis simplex parasitizing fish. Journal of Allergy and Clinical Immunology 96, 558560.
Cuéllar, C., Fenoy, S. & Guillén, J.L. (1992) Cross-reactions of sera from Toxocara canis infected mice with Toxascaris leonina and Ascaris suum antigens. International Journal for Parasitology 22, 301307.
Cuéllar, C., Fenoy, S. & Guillén, J.L. (1995) Cross-reactions of sera from Toxascaris leonina and Ascaris suum infected mice with Toxocara canis, Toxascaris leonina and Ascaris suum antigens. International Journal for Parasitology 25, 731739.
Cuéllar, C., Rodero, M., Bolás, F. & Martínez, A.R. (1997) The effects of Polypodium leocotomos extract on the specific antibody production patterns in BALB/c mice immunized with third stage larvae antigens of Anisakis simplex. International Journal of Pharmacognosy 35, 153160.
ENNA 1991 (1995) Encuesta de Presupuestos Familiares 1990–91. Estudio Nacional de Nutrición y Alimentación 1991. I.N.I., Madrid.
Flores-Romo, l., Millsum, M., Stubbs, P., Sykes, C. & Gordon, J. (1990) Immunoglobulin isotype production by cycling human B lymphocytes in response to recombinant cytokines and anti-IgM. Immunology 69, 342347.
Janeway, C. & Travers, P. (1994) Immunobiology. The immune system in health and disease. London, Current Biology Ltd.
Kasuya, S., Hamano, H. & Izumi, S. (1990) Mackerel-induced urticaria and Anisakis. Lancet 335, 665.
Mahanty, S., Day, K., Alpers, M. & Kazura, J. (1994) Antifilarial IgG4 antibodies in children from filaria-endemic areas correlate with duration of infection and are dissociated from antifilarial IgE antibodies. Journal of Infectious Diseases 170, 13391343.
Minniti, A., Micali, G., Lanuzza, F. & Panebianco, A. (1995) Determination of histamine in common sea fish parasites: Anisakis simplex and Gymnorhynchus gigas. Italian Journal of Food Science 3, 305309.
Möller, H. & Andres, K. (1986) Diseases and parasites of marine fishes. Kiel, Verlag.
Montoro, A., Perteguer, M.J., Chivato, T., Laguna, R. & Cuéllar, C. (1997) Recidivous acute urticaria caused by Anisakis simplex. Allergy 52, 985991.
Mosmann, T., Cherwinski, H., Bond, M., Giedlin, M. & Coffman, R. (1986) Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins. Journal of Immunology 136, 23482357.
Oswald, I., Gazzinelli, R., Sher, A. & James, L. (1992) IL-10 synergizes with IL-4 and transforming growth factor-β to inhibit macrophage cytotoxic activity. Journal of Immunology 148, 35783582.
Pellegrini, N., Taccini, E., Macri, B., Panebianco, A. & Gori, S. (1984) Gymnorhynchus gigas infection in Brama raji. Anatomo-histopathology and pathogenicity. Annali della Facolta di Medicina Veterinaria di Pisa 37, 191196.
Snapper, C. & Mond, J. (1993) Towards a comprehensive view of immunoglobulin class switching. Immunology Today 14, 1517.
Snapper, C., Finkelman, F. & Paul, M. (1988) Differential regulation of IgG1 and IgE synthesis by interleukin 4. Journal of Experimental Medicine 167, 183189.
Welch, J.S., Symons, M.H. & Dobson, C. (1983) Immunodiagnosis of parasitic zoonosis: purification of Toxocara canis antigens by affinity chromatography. International Journal for Parasitology 13, 171178.

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed