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Growth and development of Gnathostoma spinigerum early third-stage larvae in vitro

Published online by Cambridge University Press:  05 June 2009

W. Maleewong ,
P.M. Intapan ,
K. Ieamviteevanich ,
C. Wongkham  and
N. Morakote
W. Maleewong
Affiliation:
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
P.M. Intapan
Affiliation:
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
K. Ieamviteevanich
Affiliation:
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
C. Wongkham
Affiliation:
Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
N. Morakote
Affiliation:
Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand
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Abstract

Early third-stage larvae of Gnathostoma spinigerum were cultured in RPMI-1640 supplemented with 25mM NaHCO3,100 units/ml penicillin G, 100 μg/ml of streptomycin, 5 μg/ml of amphotericin B and 10% foetal calf serum at 37°C under 5% CO2 in air for 60 days. After 3 days of cultivation, the larvae moulted. Body sizes increased from 0.49 ± 0.09 × 0.07 ± 0.01 mm in length and width to 4.08 ± 0.48 × 0.32 ± 0.04 mm after 60 days of cultivation. The maximum body length and width of these larvae were 4.94 mm and 0.35 mm, respectively. The survival rate (67.5 %) of the worms was observed at the end of cultivation. The addition of foetal calf serum was found to be essential for growth and development.

Type
Research Note
Information
Journal of Helminthology , Volume 71 , Issue 1 , March 1997 , pp. 69 - 72
Copyright
Copyright © Cambridge University Press 1997

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