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Genotyping of Echinococcus granulosus from domestic animals and humans from Ardabil Province, northwest Iran

Published online by Cambridge University Press:  10 October 2012

A. Pezeshki ,
L. Akhlaghi ,
M. Sharbatkhori ,
E. Razmjou ,
H. Oormazdi ,
M. Mohebali  and
A.R. Meamar
A. Pezeshki
Affiliation:
Department of Parasitology and Mycology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran
L. Akhlaghi
Affiliation:
Department of Parasitology and Mycology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
M. Sharbatkhori
Affiliation:
Laboratory Science Research Center, Golestan, University of Medical Sciences, Gorgan, Iran Department of Parasitology and Mycology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran
E. Razmjou
Affiliation:
Department of Parasitology and Mycology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
H. Oormazdi
Affiliation:
Department of Parasitology and Mycology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
M. Mohebali
Affiliation:
Department of Parasitology and Mycology, School of Public health, Tehran University of Medical Sciences, Tehran, Iran
A.R. Meamar*
Affiliation:
Department of Parasitology and Mycology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
*
*Fax: +98 21 88622653 E-mail: ameamaer@sina.tums.ac.ir
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Abstract

Cystic echinococcosis is endemic in Iran, particularly in Ardabil Province, where it causes health and economic problems. The genetic pattern of Echinococcus granulosus has been determined in most parts of Iran, except in this area. In the present investigation, 55 larval isolates were collected from humans (11), sheep (19), goats (4) and cattle (21). For analysis of the genetic characteristics of E. granulosus isolates, DNA sequencing of mitochondrial cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit 1 (nad1) genes was applied. Fifty isolates were successfully analysed, with 92% (46) and 8% (4) identified as G1 and G3 genotypes, respectively. The sequence analyses of the isolates displayed nine characteristic profiles in cox1 sequences and eight characteristic profiles in nad1 sequences. Based on these results, the sheep strain (G1 genotype) was the most prevalent in humans, sheep, goats and cattle. The buffalo strain (G3 genotype) was not only demonstrated in sheep (1 isolate) and cattle (1 isolate), but also for the first time in two human isolates. These findings will provide information for local control of echinococcosis.

Type
Research Papers
Information
Journal of Helminthology , Volume 87 , Issue 4 , December 2013 , pp. 387 - 391
Copyright
Copyright © Cambridge University Press 2012 

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