Skip to main content Accessibility help
×
Home

Genetic characteristics of Chinese isolates of the tapeworm Taenia pisiformis based on two mitochondrial genes

  • D.Y. Yang (a1), Y.J. Ren (a2), Y. Fu (a1), Y. Xie (a1), X. Nong (a1), X.B. Gu (a1), S.X. Wang (a1), X.R. Peng (a3) and G.Y. Yang (a1)...

Abstract

Cysticercosis is caused by infections with embryonated eggs of the tapeworm Taenia pisiformis. Knowledge of the genetic characteristics of T. pisiformis could be applied to study the epidemiology and transmission of this parasite. In this study, 61 isolates of intraperitoneal cysticerci from eight geographically distinct regions in Sichuan province, China, were subjected to a molecular analysis in order to determine their intra-regional genetic characteristics. Partial sequences of the mitochondrial cytochrome c oxidase subunit I (cox1, 1427 bp) and NADH dehydrogenase 1 (nad1, 738 bp) were concatenated. Five haplotypes were identified, and 89.04% of total genetic variation was found in collections of T. pisiformis isolates from a single region. According to the phylogenetic reconstruction, the T. pisiformis isolates from eight regions did not form geographical clusters. Our study highlights the genetic characteristics of T.pisiformis with the aim of accelerating the genetic research and control of cysticercosis.

Copyright

Corresponding author

References

Hide All
Bagrade, G., Kirjusina, M., Vismanis, K. & Ozoliņs, J. (2009) Helminth parasites of the wolf Canis lupus from Latvia. Journal of Helminthology 83, 6368.
Baird, H.P., Miller, K.J. & Stark, J.S. (2012) Genetic population structure in the antarctic benthos: insights from the widespread amphipod, Orchomenella franklin. PLoS One 7, e34363.
Bandelt, H.J., Forster, P. & Röhl, A. (1999) Median-joining networks for inferring intraspecific phylogenies. Molecular Biology Reports 16, 3748.
Barbosa, A.M., Thode, G., Real, R., Feliu, C. & Vargas, J.M. (2012) Phylogeographic triangulation: using predator–prey–parasite interactions to infer population history from partial genetic information. PLoS One 7, e50877.
Burland, T.G. (2000) DNASTAR's Lasergene sequence analysis software. Methods in Molecular Biology 132, 7191.
Excoffier, L., Laval, G. & Schneider, S. (2007) Arlequin (version 3.0): an integrated software package for population genetics data analysis. Evolutionary Bio-informatics Online 1, 4750.
Hamrick, J.L., Godt, M.J.W. & Sherman-Broyles, L.S. (1995) Gene flow among plant populations: evidence from genetic markers. pp. 215232in Hoch, P.C. & Stephnon, A.G. (Eds) Experimental and molecular approaches to plant biosystematics. St. Louis, USA, Missouri Botanical Garden.
Huelsenbeck, J.P. & Ronquist, F. (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17, 754755.
Hüttner, M., Nakao, M., Wassermann, T., Siefert, L., Boomker, J.D., Dinkel, A., Sako, Y., Mackenstedt, U., Romig, T. & Ito, A. (2008) Genetic characterization and phylogenetic position of Echinococcus felidis (Cestoda: Taeniidae) from the African lion. International Journal for Parasitology 38, 861868.
Jeon, H.K., Kim, K.H. & Eom, K.S. (2007) Complete sequence of the mitochondrial genome of Taenia saginata: comparison with T. solium and T. asiatica. Parasitology International 56, 243246.
Jia, W.Z., Yan, H.B., Guo, A.J., Zhu, X.Q., Wang, Y.C., Shi, W.G., Chen, H.T., Zhan, F., Zhang, S.H., Fu, B.Q., Littlewood, D.T. & Cai, X.P. (2010) Complete mitochondrial genomes of Taenia multiceps, T. hydatigena and T. pisiformis: additional molecular markers for a tapeworm genus of human and animal health significance. BMC Genomics 11, 447.
Lahmar, S., Sarciron, M.E., Rouiss, M. & Mensi, M. (2008) Echinococcus granulosus and other intestinal helminths in semi-stray dogs in Tunisia: infection and re-infection rates. Tunisie Médicale 86, 657664.
Liu, G.H., Lin, R.Q., Li, M.W., Liu, W., Liu, Y., Yuan, Z.G., Song, H.Q., Zhao, G.H., Zhang, K.X. & Zhu, X.Q. (2011) The complete mitochondrial genomes of three cestode species of Taenia infecting animals and humans. Molecular Biology Reports 38, 22492256.
Martínez-Moreno, F.J., Hernández, S., López-Cobos, E., Becerra, C., Acosta, I. & Martínez-Moreno, A. (2007) Estimation of canine intestinal parasites in Córdoba (Spain) and their risk to public health. Veterinary Parasitology 143, 713.
Morjan, C.L. & Rieseberg, L.H. (2004) How species evolve collectively: implications of gene flow and selection for the spread of advantageous alleles. Molecular Ecology 13, 13411356.
Nakao, M., Xiao, N., Okamoto, M. & Yanagida, T. (2009) Geographic pattern of genetic variation in the fox tapeworm Echinococcus multilocularis. Parasitology International 58, 384389.
Neigel, J.E. & Abise, J.C. (1993) Application of random walk model to geographic distribution of animal mitochondrial DNA variation. Genetics 135, 12091220.
Rogers, A.R. & Harpending, H. (1992) Population growth makes waves in the distribution of pairwise genetic differences. Molecular Phylogenetics and Evolution 9, 552569.
Rozas, J., Sánchez-DelBarrio, J.C., Messeguer, X. & Rozas, R. (2003) DnaSP, DNA polymorphism analyses by the coalescent and other methods. Bioinformatics 19, 24962497.
Saeed, I., Maddox-Hyttel, C., Monrad, J. & Kapel, C.M. (2006) Helminths of red foxes (Vulpes vulpes) in Denmark. Veterinary Parasitology 139, 13.
Sambrook, J., Fristsch, E.F. & Maniatis, T. (2001) Molecular cloning – A laboratory manual. 3rd edn. pp. 463470. New York, Cold Spring Harbor Laboratory Press.
Tamura, K., Dudley, J., Nei, M. & Kumar, S. (2007) MEGA 4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Molecular Phylogenetics and Evolution 24, 15961599.
Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. & Higgins, D.G. (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 25, 48764882.
Tsubota, K., Nakatsuji, S., Matsumoto, M., Fujihira, S., Yoshizawa, K., Okazaki, Y., Murakami, Y., Anagawa, A., Oku, Y. & Oishi, Y. (2009) Abdominal cysticercosis in a Cynomolgus monkey. Veterinary Parasitology 161, 339341.
Yang, D.Y., Fu, Y., Wu, X.H., Xie, Y., Nie, H.M., Chen, L., Nong, X., Gu, X.B., Wang, S.X., Peng, X.R., Yan, N., Zhang, R.H., Zheng, W.P. & Yang, G.Y. (2012) Annotation of the transcriptome from Taenia pisiformis and its comparative analysis with three Taeniidae species. PLoS One 7, e32283.
Zhou, Y.X., Du, A.F., Zhang, X.J., Wu, Y.M., Tong, F.Y. & Wu, G.Y. (2008) Research on harmfulness of rabbit Cysticercus pisiformis. Journal of Zhejiang Agricultural Science 3, 372373.
Zink, R.M. & Barrowclough, G.F. (2008) Mitochondrial DNA under siege in avian phylogeography. Molecular Ecology 17, 21072121.

Metrics

Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed