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Exploring the homolog of a novel proinflammatory microfilarial sheath protein (MfP) of Wuchereria bancrofti in the adult-stage bovine filarial parasite Setaria cervi

Published online by Cambridge University Press:  27 November 2018

S. Mukherjee Open the ORCID record for S. Mukherjee [Opens in a new window] ,
N. Joardar Open the ORCID record for N. Joardar [Opens in a new window]  and
S.P. Sinha Babu Open the ORCID record for S.P. Sinha Babu [Opens in a new window]
S. Mukherjee
Affiliation:
Parasitology Laboratory, Department of Zoology (Centre for Advanced Studies), Visva-Bharati University, Santiniketan-731 235, India
N. Joardar
Affiliation:
Parasitology Laboratory, Department of Zoology (Centre for Advanced Studies), Visva-Bharati University, Santiniketan-731 235, India
S.P. Sinha Babu*
Affiliation:
Parasitology Laboratory, Department of Zoology (Centre for Advanced Studies), Visva-Bharati University, Santiniketan-731 235, India
*
Author for correspondence: S.P. Sinha Babu E-mail: spsinhababu@gmail.com
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Abstract

A novel microfilarial sheath protein (MfP) of the human filarial parasite Wuchereria bancrofti and its proinflammatory activity on host macrophages were identified recently. MfP is a homolog of the nematode bestrophin-9 superfamily that acts as a ligand of macrophage Toll-like receptor 4 (TLR4) to induce inflammation through NF-κB activation. Therefore, the presence and functional implication of this novel protein in adult-stage parasites were open questions to answer. In this study, the bovine filarial parasite Setaria cervi was used to simulate adult W. bancrofti. We detected the presence of MfP in adult-stage S. cervi through clear immunological cross-reactivity and immunolocalization employing an anti-MfP antibody developed in mice. Therefore, our findings put forward S. cervi as a cost-effective source of immunodominant filarial antigen MfP to simulate its future utilization in the immunotherapeutic intervention of lymphatic filariasis.

Keywords

filarial parasitemicrofilarial sheath protein (MfP)Setaria cervi
Type
Short Communication
Information
Journal of Helminthology , Volume 94 , 2020 , e15
Copyright
Copyright © Cambridge University Press 2018 

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Footnotes

2

Parasitology & Toxicology Laboratory, Department of Zoology, North-Eastern Hill University, Shillong-793022, Meghalaya, India (Current address)

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