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Experimental infection of selected arthropods with spirurid nematodes Spirocerca lupi Railliet & Henry, 1911 and Gongylonema ingluvicola Molin, 1857

  • S. Mukaratirwa (a1), E. Pillay (a1) and K. Munsammy (a1)


Gongylonema ingluvicola and Spirocerca lupi are spirurid nematodes that require arthropod intermediate hosts in order to complete their life cycle. Beetles of the family Scarabaeidae are reported to serve as intermediate hosts for both these parasites. In this study selected species of beetles of the family Scarabaeidae as well as other groups of arthropods were screened for susceptibility to infection with S. lupi and G. ingluvicola. Arthropods were exposed to infective eggs of both parasites for a determined period of time and dissected/digested to determine the presence or absence of pre-infective and infective larvae. All the five species of dung beetles exposed to infection with S. lupi, namely, Pachylomerus femoralis, Scarabaeus rugosus, Gymnopleurus humanus, Kheper nigroaeneus and Anachalcos convexus were susceptible and, of the two species exposed to G. ingluvicola, only Gy. humanus was susceptible. Spirocerca lupi eggs developed in millipede species, Daratoagonus cristulatus, and remained as encysted larvae, while in Orthoporoides kyrhocephalus no development was observed. Spirocerca lupi larvae were not detected in the cricket species Gryllus assimilis, or the cockroach species Periplaneta americana, and, similarly, G. ingluvicola larvae were not detected in the millipede species O. kyrhocephalus. The difference in the susceptibility of the arthropods to the two parasite species may depend on their feeding biology.


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Anantaraman, M. & Sent, K. (1966) Experimental spirocercosis in dogs with larvae from a paratenic host, Calotes versicolor, the common garden lizard in Madras. Journal of Parasitology 52, 911912.
Anderson, R.C. (1988) Nematode transmission patterns. Journal of Parasitology 74, 3045.
Bailey, W.S. (1972) Spirocerca lupi: a continuing inquiry. Journal of Parasitology 58, 322.
Bailey, W.S., Cabrera, D.J. & Diamond, D.L. (1963) Beetles of the family Scarabaeidae as intermediate hosts for Spirocerca lupi. Journal of Parasitology 49, 485488.
Chhabra, R.C. & Singh, K.S. (1973) A study of the life-history of Spirocerca lupi: intermediate hosts and their biology. Indian Journal of Animal Science 43, 4954.
Chitwood, B.G. & Chitwood, M.B. (1974) Introduction to nematology. Farnham Royal, Bucks, UK, Commonwealth Agricultural Bureau.
Dixon, K. & McCue, J.F. (1967) Further observations on the epidemiology of Spirocerca lupi in the south eastern United States. Journal of Parasitology 53, 10741075.
du Toit, C.A., Scholtz, C.H. & Nyman, W.B. (2008) Prevalence of the dog nematode Spirocerca lupi in populations of its intermediate dung beetle host in the Tshwane (Pretoria) Metropole, South Africa. Onderstepoort Journal of Veterinary Research 75, 315321.
Dvir, E., Kirberger, R.M., Mukorera, V., van der Merwe, L.L. & Clift, S.J. (2008) Clinical differentiation between dogs with benign and malignant spirocercosis. Veterinary Parasitology 155, 8088.
Faust, E.C. (1928) The life cycle of Spirocerca sanguinolenta – a natural nematode parasite of the dog. Science 68, 407409.
Fincher, G.T., Stewart, T.B. & Davis, R. (1970) Attraction of coprophagous beetles to feces of various animals. Journal of Parasitology 56, 378383.
Gorochov, A.V. & Mostovski, M.B. (2008) Apterous crickets of the tribe Gryllini from South Africa and Namibia (Orthoptera: Gryllidae). African Invertebrates 49, 109121.
Holter, P. & Scholtz, C.H. (2005) Are ball-rolling (Scarabaeini, Gymnopleurini, Sisyphini) and tunnelling scarabaeine dung beetles equally choosy about the size of ingested dung particles? Ecological Entomology 30, 700705.
Holter, P., Scholtz, C.H. & Wardhaugh, K.G. (2002) Dung feeding in adult scarabaeines (tunnellers and endocoprids): even large dung beetles eat small particles. Ecological Entomology 27, 169176.
Hopkin, S.P. & Read, H.J. (1992) The biology of millipedes. 43 pp. Oxford, Oxford University Press.
Kaufmann, J. (1996) Parasitic infections of domestic animals. 423 pp. Basel, Birkhäuser Verlag.
Knight, W.B., Hiatt, R.A., Cline, B.L. & Ritchie, L.S. (1976) A modification of the formol-ether concentration technique for increased sensitivity in detecting Schistosoma mansoni eggs. American Journal of Tropical Medicine and Hygiene 25, 818823.
Lobetti, R.G. (2000) Survey of the incidence, diagnosis, clinical manifestations and treatment of Spirocerca lupi in South Africa. Journal of South African Veterinary Association 71, 4346.
Mazaki-Tovi, M., Baneth, G., Aroch, I., Harrus, S., Kass, P.H., Ben-Ari, T., Zur, G., Aizenberg, I., Bark, H. & Lavy, E. (2002) Canine spirocercosis: clinical, diagnostic, pathologic and epidemiologic characteristics. Veterinary Parasitology 107, 235250.
McCluney, K.E. & Date, R.C. (2008) The effects of hydration on growth of the house cricket, Acheta domesticus. Journal of Insect Science 8, 19.
Miller, A. (1961) The mouth parts and digestive tract of adult dung beetles (Coleoptera: Scarabaeidae), with reference to the ingestion of helminth eggs. Journal of Parasitology 47, 735744.
Minnaar, W.N. & Krecek, R.C. (2001) Helminths of dogs belonging to people in a resource-limited urban community in Gauteng, South Africa. Onderstepoort Journal of Veterinary Research 68, 111117.
Minnaar, W.N., Krecek, R.C. & Fourie, L.J. (2002) Helminths in dogs from a peri-urban resource-limited community in Free State Province, South Africa. Veterinary Parasitology 107, 343349.
Mowlavi, G., Mikaeili, E., Mobedi, I., Kia, E., Masoomi, L. & Vatandoost, H. (2009) A survey of dung beetles infected with larval nematodes with particular notice on Copris lunaris beetles as a vector for Gongylonema sp. in Iran. Korean Journal of Parasitology 47, 1317.
Musgrave, M. (2000) Insects. pp. 2859in Carruthers, V. (Ed.) The wildlife of Southern Africa, A field guide to the animals and plants of the region. Johannesburg, South Africa, Struik Publishers.
Mwabvu, T. (1998) Food preference and coprophagy in a tropical millipede. Journal of African Zoology 112, 157161.
Permin, A., Esmann, J.B., Hoj, C.H., Hove, T. & Mukaratirwa, S. (2002) Ecto, endo and haemoparasites in free range chickens in the Goromonzi district in Zimbabwe. Preventive Veterinary Medicine 54, 213224.
Poulsen, J., Permin, A., Hindsbo, O., Yelifari, L., Nansen, P. & Bloch, P. (2000) Prevalence and distribution of gastro-intestinal helminths and haemoparsites in young scavenging chickens in upper Eastern region of Ghana, West Africa. Preventive Veterinary Medicine 45, 237245.
Seurat, L.G. (1916) Contributions a l'étude des formes larvaries des nematodes parasites heteroxenes. Bulletin Scientifique de la France et de la Belgique 49, 297377.
Soulsby, E.J.L. (1965) Textbook of veterinary clinical parasitology. pp. 88910. Oxford, Blackwell Scientific Publications.
van der Merwe, L.L., Kirberger, R.M., Clift, S., Williams, M., Keller, N. & Naidoo, V. (2008) Spirocerca lupi infection in the dog: a review. The Veterinary Journal 176, 294309.
Yamaguti, S. (1971) Systema helminthum. pp. 612614. Vol. 3, New York, USA, Interscience Publishers Inc.

Experimental infection of selected arthropods with spirurid nematodes Spirocerca lupi Railliet & Henry, 1911 and Gongylonema ingluvicola Molin, 1857

  • S. Mukaratirwa (a1), E. Pillay (a1) and K. Munsammy (a1)


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