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Evaluation of excretory/secretory products of larval Taenia solium as diagnostic antigens for porcine and human cysticercosis

Published online by Cambridge University Press:  05 June 2009

R.C. Ko  and
T.F. Ng
R.C. Ko*
Affiliation:
Department of Zoology, The University of Hong Kong, Hui Oi Chow Science Building, Pokfulam Road, Hong Kong, China
T.F. Ng
Affiliation:
Department of Zoology, The University of Hong Kong, Hui Oi Chow Science Building, Pokfulam Road, Hong Kong, China
*
*Fax: 852 2 5599114 E-mail: rcko@hkucc.hku.hk
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Abstract

Excretory/secretory antigens (ES) of larval Taenia solium were obtained by maintaining the bladder worms in Medium 199 for 3 days. Analsysis by SDS-PAGE showed that ES antigens consisted of at least 19 polypeptides, with Mrranging from 14–116 kDa. Analytical isoelectric focusing revealed eight bands with acidic pI. An immunocytolocalization study using the peroxidase method demonstrated the presence of ES epitopes on the tegument of the wall of the spiral canals of bladder worms. The specificity of ES antigens was evaluated by EITB, ELISA and FAST-ELISA using antisera against the common parasites of Chinese pigs and man. ES antigens cross-reacted with the antiserum against larval T. hydatigena of pigs. However, these antigens were generally more specific in diagnosing human cysticercosis. Three host-like molecules with molecular masses 43, 58 and 66 kDa were present in the ES products.

Type
Research Papers
Information
Journal of Helminthology , Volume 72 , Issue 2 , June 1998 , pp. 147 - 154
Copyright
Copyright © Cambridge University Press 1998

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References

Cheng, R.W.K. & Ko, R.C. (1991) Cross-reactions between crude antigens of larval Taenia solium and other helminths of pigs. Veterinary Parasitology 39, 161–170.CrossRefGoogle ScholarPubMed
Diaz, F., Garcia, H., Gilman, R.H., Gonzles, A.E., Castron, M., Tsang, V.C.W., Pilcher, J.B., Vasquez, L.E., Lescano, M., Carcamo, C., Madico, G., Miranda, E. & The Cysticercosis Working Group in Peru (1992) Epidemiology of taeniasis and cysticercosis in a Peruvian village. American Journal of Epidemiology 135, 875–882.Google Scholar
Ellis, L.A., Reason, A.J., Morris, H.R., Dell, A., Iglesias, R., Ubeira, F.M. & Appleton, L.A. (1994) Glycans as targets for monoclonal antibodies that protect rats against Trichinella spiralis. Glycobiology 4, 585–592.Google Scholar
Fagbemi, B.O. & Hillyer, G.V. (1990) Immunodiagnosis of dracunculiasis by falcon assay screening test-enzyme-linked immunosorbent assay (FAST-ELISA) and by enzyme-linked immunoelectrotransfer blot (EITB) technique. American Journal of Tropical Medicine and Hygiene 43, 665–668.CrossRefGoogle ScholarPubMed
Gonzalez, A.E., Cama, V., Gilman, R.H., Tsang, V.C., Pilcher, J.B., Chavera, A., Castro, M., Montenegro, T., Verastegui, M., Miranda, E. & Bazalar, H. (1990) Prevalence and comparison of serologic assays, necropsy, and tongue examination for the diagnosis of porcine cysticercosis in Peru. American Journal of Tropical Medicine and Hygiene 43, 194–199.CrossRefGoogle ScholarPubMed
Hancock, K. & Tsang, V.C.W. (1986) Development and optimization of the FAST-ELISA for detecting antibodies to Schistosoma mansoni. Journal of Immunology 92, 167–176.Google ScholarPubMed
Joshua, G.W.P., Harrison, L.J.S. & Sewell, M.M.H. (1990) Protein antigens in the cyst fluid of Taenia saginata cysticerci. Parasitology 100, 463–467.Google Scholar
Kamango-Sollo, E.I.P., Lindquist, K.J. & Gathuma, J.M. (1985) Bovine serum components in Taenia saginata cysticerci (Cysticercus bovis). Bulletin of Animal Health and Products of Africa 33, 1–3.Google Scholar
Ko, R.C. & Yeung, M.H.F. (1989) Specificity of ES antigens in detection of Trichinella spiralis antibodies in Chinese pigs. Tropical Biomedicine 6, 99–111.Google Scholar
Ko, R.C. & Yeung, M.H.F. (1991) Isolation of specific antigens from Trichinella spiralis by the rotating horizontal ampho line column method. Parasitology Research 77, 255–259.Google Scholar
Kwa, B.H. & Liew, F.Y. (1978) Studies on the mechanism of long term survival of Taenia taeniaeformis in rats. Journal of Helminthology 52, 1–6.CrossRefGoogle ScholarPubMed
Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.CrossRefGoogle ScholarPubMed
Landa, A., Merchant, M.T., Willms, K. & Laclette, J.P. (1994) Purification and ultrastructural localization of surface glycoproteins of Taenia solium (Cestoda) cysticerci. International Journal for Parasitology 24, 265–269.Google Scholar
Machnicka, B. & Grzybowski, J. (1986) Host serum proteins in Taenia saginata metacestode fluid. Veterinary Parasitology 19, 7–54.Google Scholar
Pammenter, M.D., Efstein, S.R. & Rees, R.T. (1992) Cross-reactions in the immunodiagnosis of schistosomiasis and cysticercosis by a cerebrospinal fluid enzyme-linked immunosorbent assay. Transactions of the Royal Society of Tropical Medicine and Hygiene 86, 51–52.Google Scholar
Plancarte, A., Fexas, M. & Flisser, A. (1994) Reactivity in ELISA and dot-blot of GP24, an immunodominant antigens of Taenia solium, for the diagnosis of human cysticercosis. International Journal for Parasitology 24, 733–738.Google Scholar
Reason, A.J., Ellis, L.A., Appleton, J.A., Widnewski, N., Grieve, R.B., McNeil, M., Wassom, D.L., Morris, H.R. & Dell, A. (1994) Novel tyvelose-containing tri- and tetraantennary N-glycans in the immuno-dominant antigens of the intracellular parasite Trichinella spiralis. Glycobiology 4, 593–603.Google Scholar
Tsang, V.C.W., Brand, J.A. & Boyer, A.E. (1989) An enzyme-linked immunoelectrotransfer blot assay and glycoprotein antigens for diagnosing human cysticercosis (Taenia solium). Journal of Infectious Disease 159, 50–59.CrossRefGoogle ScholarPubMed
Vidor, E., Piens, M.A. & Garin, J.P. (1987) Host serum protein levels in cysts of human hydatidosis. Transactions of the Royal Society of Tropical Medicine and Hygiene 81, 699–671.CrossRefGoogle ScholarPubMed
Willms, K., Laclette, J.P., Arcos, L. & Merchant, M. (1986) The origin of porcine IgG epitopes on the external surface of Taenia solium metacestodes. Abstracts of the Sixth International Congress of Parasitology, Brisbane, Australia, p. 258.Google Scholar