Hostname: page-component-8448b6f56d-42gr6 Total loading time: 0 Render date: 2024-04-20T14:56:53.668Z Has data issue: false hasContentIssue false

Efficacy of the nematode-trapping fungus Duddingtonia flagrans against infections by Haemonchus and Trichostrongylus species in lambs at pasture

Published online by Cambridge University Press:  01 December 2007

R.A. Rocha*
Affiliation:
UNESP, São Paulo State University, Instituto de Biociências, Departamento de Parasitologia, Caixa Postal 510, CEP 18618-000, Botucatu-SP, Brazil
J.V. Araújo
Affiliation:
Departamento de Veterinária, Universidade Federal de Viçosa, CEP 36570-000, Viçosa, MG, Brazil
A.F.T. Amarante
Affiliation:
UNESP, São Paulo State University, Instituto de Biociências, Departamento de Parasitologia, Caixa Postal 510, CEP 18618-000, Botucatu-SP, Brazil
*
*Author for correspondence E-mail: rrabdallah@hotmail.com

Abstract

The efficacy of the nematode-trapping fungus Duddingtonia flagrans against infections by trichostrongyle nematodes in sheep was assessed throughout 6 months. Twenty Ile de France lambs were divided into two groups (control and treated groups), which were kept in separate pastures. Animals of the treated group were fed with D. flagrans twice a week (Tuesdays and Fridays). Pellets were prepared with the fungus mycelia in liquid culture medium and contained approximately 20% fungus. They were mixed with the animals' diet at a concentration of 1 g pellet per 10 kg live weight. Faecal egg counts (FEC), packed cell volume (PCV), total serum protein and the animals' body weight were determined fortnightly from 7 October 2005 to 24 March 2006. Comparison of such parameters between groups showed no significant differences (P > 0.05), except on 10 February 2006, when the control group presented a higher mean FEC than the treated group (P <  0.05). Feeding sheep with pellets containing D. flagrans had no benefit to the prophylaxis of nematode infections under the experimental conditions used in the present study.

Type
Research Papers
Copyright
Copyright © Cambridge University Press 2007

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Alves, P.H., Araújo, J.V., Guimarães, M.P., Assis, R.C.L., Sarti, P. & Campos, A.K. (2003) Aplicação de formulação do fungo predador de nematóides Monacrosporium thaumasium (Dreschsler, 1937) no controle de nematóides de bovinos. Arquivo Brasileiro de Medicina Veterinária e Zootecnia 55, 568573.Google Scholar
Amarante, A.F.T. & Barbosa, M.A. (1995) Seasonal variations in populations of infective larvae on pasture and nematode faecal egg output in sheep. Veterinária e Zootecnia 7, 127133.Google Scholar
Amarante, A.F.T., Craig, T.M., Ramsey, W.S., El-Sayed, N.M., Desouki, A.Y. & Bazer, F.W. (1999) Comparison of naturally acquired parasite burdens among Florida Native, Rambouillet and crossbreed ewes. Veterinary Parasitology 85, 6169.Google Scholar
Araújo, J.V., Guimarães, M.P., Campos, A.K., , N.C., Sarti, P. & Assis, R.C.L. (2004) Control of bovine gastrointestinal nematode parasites using pellets of the nematode-trapping fungus Monacrosporium thaumasium. Ciência Rural 34, 457463.CrossRefGoogle Scholar
Araújo, J.V., Freitas, B.W., Vieira, T.C. & Campos, A.K. (2006) Avaliação do fungo predador de nematóides Duddingtonia flagrans sobre larvas infectantes de Haemonchus contortus e Strongyloides pappillosus de caprinos. Revista Brasileira de Parasitologia Veterinária 15, 7679.Google Scholar
Chandrawathani, P., Jamnah, O., Waller, P.J., Larsen, M., Gillespie, A.T. & Zahari, W.M. (2003) Biological control of nematode parasites of small ruminants in Malaysia using the nematophagous fungus Duddingtonia flagrans. Veterinary Parasitology 117, 173183.Google Scholar
Duddington, C.L. (1955) Notes on the technique of handling predaceous fungi. Transactions of the British Mycological Society 38, 97103.Google Scholar
Eysker, M., Bakker, N., Kooyman, F.N.J., Olthuis, S.O. & Ploeger, H.W. (2006) Effect of biological control through the daily application of spores of Duddingtonia flagrans in lambs kept under an evasive grazing system in the Netherlands. Veterinary Parasitology 140, 312320.Google Scholar
Faedo, M., Barnes, E.H., Dobson, R.J. & Waller, P.J. (1998) The potential of nematophagous fungi to control the free-living stages of nematode parasites of sheep: Pasture plot study with Duddingtonia flagrans. Veterinary Parasitology 76, 129135.CrossRefGoogle ScholarPubMed
Fontenot, M.E., Miller, J.E., Peña, M.T., Larsen, M. & Gillespie, A. (2003) Efficiency of feeding Duddingtonia flagrans chlamydospores to grazing ewes on reducing availability of parasitic nematode larvae on pasture. Veterinary Parasitology 118, 203213.CrossRefGoogle ScholarPubMed
Gómez-Rincón, C., Uriarte, J. & Valderrábano, J. (2006) Efficiency of Duddingtonia flagrans against Trichostrongyle infections of sheep on mountain pastures. Veterinary Parasitology 141, 8490.Google Scholar
Graminha, E.B.N., Monteiro, A.C., Silva, H.C., Oliveira, G.P. & Costa, A.J. (2005) Controle de nematóides parasitos gastrintestinais por Arthrobotrys musiformis em ovinos naturalmente infestados mantidos em pastagens. Pesquisa Agropecuária Brasileira 40, 927933.Google Scholar
Keith, R.K. (1953) The differentiation of infective larvae of some common nematode parasites of cattle. Australian Journal of Zoology 1, 223235.CrossRefGoogle Scholar
Lackey, B.A., Muldoon, A.E. & Jaffe, B.A. (1993) Alginate pellet formulation of Hirsutella rossiliensis for biological control of plant-parasitic nematodes. Biological Control 3, 155160.Google Scholar
Larsen, M. (1999) Biological control of helminths. International Journal for Parasitology 29, 139146.Google Scholar
Larsen, M., Faedo, M., Waller, P.J. & Hennessy, D.R. (1998) The potential of nematophagous fungi to control the free-living stages of nematode parasites of sheep: studies of Duddingtonia flagrans. Veterinary Parasitology 76, 121128.CrossRefGoogle ScholarPubMed
Paraud, C., Cabaret, J., Pors, I. & Chartier, C. (2005) Impact of the nematophagous fungus Duddingtonia flagrans on Muellerius capillaries larvae in goat faeces. Veterinary Parasitology 131, 7178.CrossRefGoogle Scholar
Roberts, F.H.S. & O'Sullivan, S.P. (1950) Methods for egg counts and larval cultures for strongyles infesting the gastrointestinal tract of cattle. Australian Journal of Agricultural Research 1, 99102.Google Scholar
Santos, M.A., Ferraz, S. & Muchovej, J. (1991) Detection and ecology of nematophagous fungi from Brazilian soils. Nematologia Brasileira 15, 121134.Google Scholar
Taylor, E.L. (1939) Technique for the estimation of pasture infestation by strongyloid larvae. Parasitology 31, 473478.CrossRefGoogle Scholar
Tembely, S., Lahlou-Kassi, A., Rege, J.E.O., Mukasa-Mugerwa, E., Anindo, D., Sovani, S. & Baker, R.L. (1998) Breed and season effects on periparturient rise in nematode egg output in indigenous ewes in a cool tropical environment. Veterinary Parasitology 77, 123132.CrossRefGoogle Scholar
Terrill, T.H., Larsen, M., Samples, O., Husted, S., Miller, J.E., Kaplan, R.M. & Gelaye, S. (2004) Capability of the nematode-trapping fungus Duddingtonia flagrans to reduce infective larvae of gastrointestinal nematodes in goat feces in the southeastern United States: dose titration and dose time interval studies. Veterinary Parasitology 120, 285296.Google Scholar
Walker, H.L. & Connick, W.J. (1983) Sodium alginate for production and formulation of mycoherbicides. Weed Science 31, 333338.CrossRefGoogle Scholar
Waller, P.J. (2006) From discovery to development: Current industry perspectives for the development of novel methods of helminth control in livestock. Veterinary Parasitology 139, 114.Google Scholar
Waller, P.J., Knox, M.R. & Faedo, M. (2001) The potential of nematophagous fungi to control the free-living stages of nematode parasites of sheep: feeding and block studies with Duddingtonia flagrans. Veterinary Parasitology 102, 321330.Google Scholar