Skip to main content Accessibility help

Echinococcus granulosus strain differentiation in Iran based on sequence heterogeneity in the mitochondrial 12S rRNA gene

  • M. Rostami Nejad (a1), E. Nazemalhosseini Mojarad (a1), Z. Nochi (a1), M. Fasihi Harandi (a2), K. Cheraghipour (a3), G.R. Mowlavi (a4) and M.R. Zali (a1)...


Parasite strain characterization is essential for the establishment of a prevention and control strategy in any endemic area. The aim of this study was to characterize different Echinococcus granulosus isolates from Iran by using DNA sequences of the mitochondrial 12S rRNA gene. Thirty livers and lungs of cattle, sheep and goats naturally infected with E. granulosus were collected from abattoirs in northern and western Iran between June and October 2007. These samples yielded 18 fertile cysts which we used for the genetic work. We designed and tested two new primer pairs which specifically amplify portions of the mitochondrial 12S rRNA gene of the two strains (G1 and G6) of E. granulosus known to occur in Iran. One primer pair amplified a fragment of 259 base pairs (bp) from only the G1 strain. The second pair amplified a fragment of 676 bp from the G6 strain. The G1 genotype was identified in all fertile cyst samples, in agreement with previous studies in Iran. Ten of our samples and a single reference sample of the G6 strain were sequenced and compared with the G1 and G6 sequences deposited in GenBank.


Corresponding author

*Fax: +98 21 22432517 E-mail:


Hide All
Ahmadi, N. & Dalimi, A. (2006) Characterization of Echinococcus granulosus isolates from human, sheep and camel in Iran. Infection, Genetics and Evolution 6, 8590.
Bowles, J., Blair, D. & McManus, D.P. (1992) Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Molecular and Biochemical Parasitology 54, 169174.
Craig, P.S., Rogan, M.T. & Allan, J.C. (1996) Detection, screening and community epidemiology of taeniid cestode zoonoses in cystic echinococcosis, alveolar echinococcosis and neurocysticercosis. Advances in Parasitology 38, 169250.
Dalimi, A., Motamedi, Gh., Hosseini, M., Mohammadian, B., Malaki, H., Ghamari, Z. & Ghaffari-far, F. (2002) Echinococcosis/hydatidosis in Western Iran. Veterinary Parasitology 105, 161171.
Euzeby, J. (1991) The epidemiology of hydatidosis with special reference to the Mediterranean area. Parasitologia 33, 2539.
Fasihi Harandi, M., Hobbs, R.P., Adams, P.J., Mobedi, I., Morgan-Ryan, U.M. & Thompson, R.C.A. (2002) Molecular and morphological characterization of Echinococcus granulosus of human and animal origin in Iran. Parasitology 125, 367373.
McManus, D.P. & Thompson, R.C.A. (2003) Molecular epidemiology of cystic echinococcosis. Parasitology 127, S37S51.
Mehrabani, D., Oryan, A. & Sadjjadi, S.M. (1999) Prevalence of Echinococcus granulosus infection in stray dogs and herbivores in Shiraz, Iran. Veterinary Parasitology 86, 217220.
Mobedi, I., Madadi, H. & Arfaa, F. (1970) Camel, Camelus dromedarius, as intermediate host of Echinococcus granulosus in Iran. Journal of Parasitology 56, 1255.
Moghaddar, N., Oryan, A. & Hanifpour, M.R. (1992) Helminthes recovered from the liver and lungs of camel with special reference to their incidence and pathogenesis in Shiraz, Islamic Republic of Iran. Indian Journal of Animal Science 62, 10181023.
Oryan, A., Moghadar, N. & Gaur, S.N.S. (1994) Metacestodes of sheep with special reference to their epidemiological status, pathogenesis and economic implications in Fars province, Iran. Veterinary Parasitology 51, 231240.
Pearson, M., Le, T.H., Zhang, L.H., Blair, D., Dai, T.H.N. & McManus, D.P. (2002) Molecular taxonomy and strain analysis in Echinococcus. pp. 205219in Craig, P. & Pawlowski, Z. (Eds) Cestode Zoonoses: Echinococcosis and Cysticercosis. An emergent and global problem. NATO Science Series. Vol. 341, Amsterdam, IOS Press.
Rahimi, H.R., Kia, E.B., Mirhendi, S.H., Talebi, A., Fasihi Harandi, M., Jalali-zand, N. & Rokni, M.B. (2007) A new primer pair in ITS1 region for molecular studies on Echinococcus granulosus. Iranian Journal of Public Health 36, 4549.
Rostami Nejad, M., Hoseinkhan, N., Nazemalhosseini, E., Cheraghipour, K., Abdinia, E. & Zali, M.R. (2007a) An analysis of hydatid cyst surgeries in patients referred to hospitals in Khorram-Abad, Lorestan during 2002–06. Iranian Journal of Parasitology 3, 2933.
Rostami Nejad, M., Abdinia, E. & Cheraghipour, K. (2007b) A survey on liver and lung hydatid cyst of slaughtered animals in Aleshtar. Lorestan province of Iran during 2002-06. Journal of Yasoj University of Medical Science 2, 59 (in Persian).
Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular cloning, a laboratory manual. 2nd ed.New York, Cold Spring Harbor Laboratory Press.
Schantz, P. (1997) Echinococcus granulosus, E. multilocularis, and E. vogeli (agents of cystic, alveolar, and polycystic Echinococcosis). pp. 14881492in Long, S.S. & Prober, C.G. (Eds) Pediatric infectious diseases. New York, Churchill Livingstone.
Scott, J.C., Stefaniak, J., Pawlowski, Z.S. & McManus, D.P. (1996) Molecular genetic analysis of human cystic hydatid cases from Poland: Identification of a new genotypic group (G9) of Echinococcus granulosus. Parasitology 114, 3743.
Thompson, R.C.A. (2001) Echinococcosis. pp. 595612in Gillespie, S.H. & Pearson, R.D. (Eds) Principles and practice of clinical parasitology. Sussex, Wiley.
Zhang, L.H., Eslami, A., Hosseini, S.H. & McManus, D.P. (1998) Indication of the presence of two distinct strains of E. granulosus in Iran by mitochondrial DNA markers. American Journal of Medical Hygiene 59, 171174.

Related content

Powered by UNSILO

Echinococcus granulosus strain differentiation in Iran based on sequence heterogeneity in the mitochondrial 12S rRNA gene

  • M. Rostami Nejad (a1), E. Nazemalhosseini Mojarad (a1), Z. Nochi (a1), M. Fasihi Harandi (a2), K. Cheraghipour (a3), G.R. Mowlavi (a4) and M.R. Zali (a1)...


Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed.