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Echinococcus granulosus from Mexican pigs is the same strain as that in Polish pigs

  • A. Cruz-Reyes (a1), C.C. Constantine (a2), A.C. Boxell (a3), R.P. Hobbs (a3) and R.C.A. Thompson (a3)...

Abstract

Samples of Echinococcus granulosus from seven pigs from Mexico were compared with isolates of the parasite from pigs in Poland and representative strains and species of Echinococcus. Isolates from pigs in Mexico were found to be genetically identical to E. granulosus from Polish pigs and distinct from other major genotypes by sequencing part of the mitochondrial cytochrome c oxidase I (COI) mtDNA locus, restriction fragment length polymorphism (RFLP) of the polymerase chain reaction (PCR) amplified rDNA internal transcribed spacer (ITS) 1 using five different enzymes, and random amplified polymorphic DNA (RAPD) analysis. These results were complemented by data on hook morphology and together strengthen the view that Echinococcus maintained in a cycle involving pigs and dogs is a distinct strain that is conserved genetically in different geographical areas. The present study supports the close relationship of the cervid, camel and pig strains and raises the question of their taxonomic status.

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Corresponding author

*Fax: +61 89310 4144 E-mail: a.thompson@murdoch.edu.au

References

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Bowles, J. & McManus, D.P. (1993) Rapid discrimination of Echinococcus species and strains using a polymerase chain reaction-based RFLP method. Molecular and Biochemical Parasitology 57, 231240.
Bowles, J., Blair, D. & McManus, D.P. (1992) Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Molecular and Biochemical Parasitology 54, 165174.
Cruz-Reyes, A. & Martinez-Lopez, A. (1990) Desarrollo de Echinococcus granulosus a partir de quistes de cerdos infectados naturalmente, Zacatecas, Mexico. Revista Mexicana de Parasitologia 3, 359.
Eckert, J., Thompson, R.C.A., Michael, S.A., Kumaratilake, L.K. & El-Sawah, H.M. (1989) Echinococcus granulosus of camel origin: development in dogs and parasite morphology. Parasitology Research 75, 536544.
Eckert, J., Schantz, P.M., Gasser, R.B., Torgerson, P.R., Bessonov, A.S., Movsessian, S.O., Thakur, A., Grimm, F. & Nikogossian, M.A. (2001) Geographic distribution and prevalence. pp. 101–143 in Eckert, J., Gemmell, M.A., Meslin, F.-X. & Pawlowski, Z.S. (Eds) Manual on Echinococcus in humans and animals a public health problem of global concern. Paris, WHO/OIE; Geneva, World Health Organisation.
Harandi, F.M., Hobbs, R.P., Adams, P.J., Mobedi, I., Morgan-Ryan, U.M. & Thompson, R.C.A. (2002) Molecular and morphological characterisation of Echinococcus granulosus of human and animal origin in Iran. Parasitology 125, 367373.
Hobbs, R.P., Lymbery, A.J. & Thompson, R.C.A. (1990) Rostellar hook morphometry of Echinococcus granulosus (Batsch, 1786) from natural and experimental Australian hosts, and its implications for strain recognition. Parasitology 101, 273–281.
Kumaratilake, L.M., Thompson, R.C.A. & Eckert, J. (1986) Echinococcus granulosus of equine origin from different countries possess uniform morphological characteristics. International Journal for Parasitology 16, 529540.
Lavikainen, A., Lehtinen, M.J., Meri, T., Hirvelä-Koski, V. & Meri, S. (2003) Molecular genetic characterisation of the Fennoscandian cervid strain, a new genotypic group (G10) of Echinococcus granulosus. Parasitology 127, 207–215.
Lavikainen, A., Lehtinen, M.J., Laakonen, S., Agren, E., Oksanen, A. & Meri, S. (2006) Molecular characterisation of Echinococcus isolates of cervid origin from Finland and Sweden. Parasitology 133, 565570.
Lopez-Neyra, C.R. & Soler Planas, M.A. (1943) Revision del genero Echinococcus Rud y description de una especie nuéva Parasita intestinal del porro en Almeria. Revista Ibérica Parasitologia 3, 169–194.
Martinez-Lopez, A., Jaramillo, C. & Cruz-Reyes, A. (1990) Equinococcosis/hidatidosis, un estudio retrospectivo en Mexico. Revista Mexicana de Parasitologia 3, 362.
Maravilla, P., Thompson, R.C.A., Palacios-Ruiz, J.A., Estcourt, A., Ramirez-Solis, E., Mondragon-de-la-Pena, C., Moreno-Miller, M., Cardenas-Mejia, A., Mata-Miranda, P., Aguirre-Alcantara, M-T., Bonilla-Rodriguez, C. & Flisser, A. (2004) Echinococcus granulosus cattle strain identification in an autochthonous case of cystic echinococcosis in central Mexico. Acta Tropica 92, 231236.
McManus, D.P. & Thompson, R.C.A. (2003) Molecular epidemiology of cystic echinococcosis. Parasitology 127, S37–S51.
Obwaller, A., Schneider, R., Walochnik, J., Gollackner, B., Deutz, A., Janitschke, K., Aspöck, H. & Auer, H. (2004) Echinococcus granulosus strain differentiation based on sequence heterogeneity in mitochondrial genes of cytochrome c oxidase-1 and NADH dehydrogenase-1. Parasitology 128, 569575.
Salinas-López, N., Jiménez-Guzmán, F. & Cruz-Reyes, A. (1996) Presence of Echinococcus oligarthrus (Diesing, 1863) Lühe, 1910 in Lynx rufus texensis Allen, 1895 from San Fernando, Tamaulipas State, in North-East Mexico. International Journal for Parasitology 26, 793796.
Schantz, P.M., Chai, J., Craig, P.S., Eckert, J., Jenkins, D.J., Macpherson, C.N.L. & Thakur, A. (1995) Epidemiology and control of hydatid disease. pp. 233–331 in Thompson, R.C.A. & Lymbery, A.J. (Eds) Echinococcus and hydatid disease. CAB International, Wallingford, Oxon, UK.
Sweatman, G.K. & Williams, R.J. (1963) Comparative studies on biology and morphology of. Echinococcus granulosus from domestic livestock, moose and reindeer. Parasitology 53, 339–390.
Tajima, F. & Nei, M. (1984) Estimation of evolutionary distance between nucleotide sequences. Molecular Biology and Evolution 1, 269285.
Thompson, J.D., Higgins, D.G. & Gibsan, T.J. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight martix choice. Nucleic Acids Research 22, 46734680.
Thompson, R.C.A. (2001) Echinococcosis. pp. 595612in Gillespie, S.H. & Pearson, R.D. (Eds) Principles and practices of clinical parasitology. Wiley, Sussex, UK.
Thompson, R.C.A. & Lymbery, A.J. (1988) The nature, extent and significance of variation within the genus Echinococcus. Advances in Parasitology 27, 210–263.
Thompson, R.C.A. & McManus, D.P. (2001) Aetiology: parasites and life cycles. pp. 1–19 in Eckert, J., Gemmell, M.A., Meslin, F.-X. & Pawlowski, Z.S. (Eds) Manual on Echinococcus in humans and animals a public health problem of global concern. Paris, WHO/OIE; Geneva, World Health Organisation.
Thompson, R.C.A. & McManus, D.P. (2002) Towards a taxonomic revision of the genus Echinococcus. Trends in Parasitology 18, 452457.
Thompson, R.C.A., Lymbery, A.J. & Constantine, C.C. (1995) Variation in Echinococcus: towards a taxonomic revision of the genus. Advances in Parasitology 35, 145176.
Thompson, R.C.A., Boxell, A.C., Ralston, B.J., Constantine, C.C., Hobbs, R.P., Shury, T. & Olson, M.E. (2006) Molecular and morphological characterisation of Echinococcus in cervids from North America. Parasitology 132, 439–447.
Van De Peer, Y. & De Wachter, R. (1993) TREECON: a software package for the construction and drawing of evolutionary trees. Computational Applications for Biosciences 9, 177182.
Vargas-Rivera, I., Martinez-Maya, J.J. & Jaramillo-Arango, C.J. (1995) Epidemiology of porcine hydatidosis at the abattoir of Los Paz in Mexico. Veterinaria Mexico 26, 365–368.
Xiao, N., Qiu, J., Nakao, M., Tiaoying, L., Yang, W., Chen, X., Schautz, P.M., Craig, P.S. & Ito, A. (2005) Echinococcus shiquicus n. sp., a taeniid cestode from Tibotan fox and plateau pika in china. International Journal for Parasitology 35, 693701.

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