Skip to main content Accessibility help
×
Home

Duddingtonia flagrans, Monacrosporium thaumasium and Pochonia chlamydosporia as possible biological control agents of Oxyuris equi and Austroxyuris finlaysoni

  • F.R. Braga (a1), J.V. Araújo (a1), A.R. Silva (a1), J.M. Araujo (a1), R.O. Carvalho (a1), A.K. Campos (a2), A.O. Tavela (a1), S.R. Ferreira (a1), L.N. Frassy (a1) and C.D.F. Alves (a1)...

Abstract

The action of four fungal isolates of the species Duddingtonia flagrans (AC001), Monacrosporium thaumasium (NF34a) and Pochonia chlamydosporia (VC1 and VC4) on eggs of Oxyuris equi and Austroxyuris finlaysoni was evaluated in two assays (A and B). Eggs of O. equi (Test A) and A. finlaysoni (Test B) were plated on Petri dishes with 2% water-agar with grown fungal isolates and control without fungus. After 5, 10 and 15 days, 100 eggs were collected and classified according to the following parameters: type 1 effect, physiological and biochemical effect without morphological damage to the eggshell; type 2 effect, lytic effect with morphological alteration of the eggshell and embryo; and type 3 effect, lytic effect with morphological alteration of the eggshell and embryo, hyphal penetration and internal egg colonization. Pochonia chlamydosporia isolates VC1 and VC4 showed ovicidal activity for type 1, 2 and 3 effects on eggs of O. equi and eggs of A. finlaysoni. In vitro assays A and B showed that P. chlamydosporia had a negative influence on eggs of O. equi and A. finlaysoni and can be considered as a potential biological control agent of nematodes.

Copyright

Corresponding author

References

Hide All
Araújo, J.V., Santos, M.A. & Ferraz, S. (1995) Efeito ovicida de fungos nematófagos sobre ovos embrionados de Toxocara canis. Arquivo Brasileiro de Medicina Veterinária e Zootecnia 47, 3242.
Araújo, J.V., Mota, M.A. & Campos, A.K. (2004) Controle biológico de helmintos parasitos de animais por fungos nematófagos. Revista Brasileira de Parasitologia Veterinária 13, 165170.
Araújo, J.V., Braga, F.R., Araújo, J.M., Silva, A.R. & Tavela, A.O. (2008) In vitro evaluation of the effect of the nematophagous fungi Duddingtonia flagrans, Monacrosporium sinense and Pochonia chlamydosporia on Ascaris suum eggs. Parasitology Research 102, 787790.
Araújo, J.M., Araújo, J.V., Braga, F.R., Carvalho, R.O., Silva, A.R. & Campos, A.K. (2009) Interaction and ovicidal activity of nematophagous fungus Pochonia chlamydosporia on Taenia saginata eggs. Experimental Parasitology 121, 338341.
Ayres, M., Ayres, J.R.M., Ayres, D.L. & Santos, A.S. (2003) Aplicações estatísticas nas áreas de ciências biológicas. 290 pp. Brasília, CNPq.
Bonuti, M.R., Nascimento, A.A., Mapeli, E.B. & Arantes, I.G. (2002) Gastrointestinal helminths of capybara (Hydrochoerus hydrochaeris) from the Paiaguás subregion, in the floodplain of Mato Grosso do sul, Brasil. Ciências Agrárias 23, 5762.
Bowman, D.D., Lynn, R.C., Eberhard, M. & Alacaraz, L. (2006) Parasitologia Veterinária de Georgis. pp. 115130. 8th edn.Rio de Janeiro, Editora Manole.
Braga, F.R., Araújo, J.V., Campos, A.K., Carvalho, R.O., Silva, A.R., Tavela, A.O. & Maciel, A.S. (2007) Observação in vitro da ação dos isolados fúngicos Duddingtonia flagrans, Monacrosporium thaumasium e Verticillium chlamydosporium sobre ovos de Ascaris lumbricoides (Lineu, 1758). Revista da Sociedade Brasileira de Medicina Tropical 40, 356358.
Braga, F.R., Araújo, J.V., Campos, A.K., Carvalho, R.O., Silva, A.R. & Tavela, A.O. (2008) In vitro evaluation of the effect of the nematophagous fungi Duddingtonia flagrans, Monacrosporium sinense and Pochonia chlamydosporia on Fasciola hepatica eggs. World Journal of Microbiology and Biotechnology 24, 15591564.
Burger, H.J. & Stoye, M. (1978) Parasitological problems associated with recycling of animal excretions. In Kelly, W.R. (Ed.) Animal and human hazards associated with the utilization of animal effluents. Luxembourg, Office for the Official Publications of the European Communities.
Gams, W. & Zare, R. (2001) A revision of Verticillium sect. Prostrata. III. Generic classification. Nova Hedwigia 73, 329337.
Gomes, D.C., Cruz, R.P. & Vicente, J.J. (2003) Nematode parasites of marsupials and small rodents from the Brazilian Atlantic Forest in the State of Rio de Janeiro, Brazil. Revista Brasileira de Zoologia 20, 699707.
Hugot, J.P. & Bougnoux, M.E. (1988) Estude morphologique de Austroxyuris finlaysoni (Oxyuridae, Nematoda), parasite de Petauroides volans (Petauridae, Marsupiala). Systematic Parasitology 11, 113122.
Larsen, M. (1999) Biological control of helminths. International Journal for Parasitology 29, 139146.
Lysek, H. & Sterba, J. (1991) Colonization of Ascaris lumbricoides eggs by the fungus Verticillium chlamydosporium Goddard. Folia Parasitologica 38, 255259.
Lysek, H., Fassatiová, O., Pineda, N.C. & Hernández, N.L. (1982) Ovicidal fungi in soils from Cuba. Folia Parasitologica 29, 265270.
Morgan-Jones, G., White, J.F. & Rodriguez-Kábana, R. (1983) Phytonematode pathology: ultrastructural studies. I: Parasitism of Meloidogyne arenaria eggs by Verticillium chlamydosporium. Nematropica 13, 245260.
Nordbring-Hertz, B., Jansson, H.B. & Tunlid, A. (2002) Nematophagous fungi. Encyclopedia of life sciences. 10 pp. Basingstoke, Macmillan Publishers.
Sanyal, P.K., Sarkar, A.K., Patel, N.K., Mandal, S.C. & Pal, S. (2008) Formulation of a strategy for the application of Duddingtonia flagrans to control caprine parasitic gastroenteritis. Journal of Helminthology 82, 169174.
Silva, A.V.M. & Massara, C.L. (2005) Ascaris lumbricoides. pp. 253259 in Neves, D.P. (Ed.) Parasitologia humana. Rio de Janeiro, Atheneu.
Stirling, G.R. & West, L.M. (1991) Fungal parasites of root-knot nematodes eggs from tropical and sub tropical regions of Australia. Australasian Plant Pathology Society 20, 149154.
Urquhart, G.M., Armour, J., Duncan, J.L., Dunn, A.M. & Jennings, F.W. (1998) Parasitologia Veterinária. 273 pp. 2nd edn.Rio de Janeiro, Guanabara & Koogan.

Duddingtonia flagrans, Monacrosporium thaumasium and Pochonia chlamydosporia as possible biological control agents of Oxyuris equi and Austroxyuris finlaysoni

  • F.R. Braga (a1), J.V. Araújo (a1), A.R. Silva (a1), J.M. Araujo (a1), R.O. Carvalho (a1), A.K. Campos (a2), A.O. Tavela (a1), S.R. Ferreira (a1), L.N. Frassy (a1) and C.D.F. Alves (a1)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed