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Dogs are reservoir hosts for possible transmission of human strongyloidiasis in Thailand: molecular identification and genetic diversity of causative parasite species

Published online by Cambridge University Press:  17 December 2019

O. Sanpool Open the ORCID record for O. Sanpool [Opens in a new window] ,
P.M. Intapan ,
R. Rodpai ,
P. Laoraksawong ,
L. Sadaow ,
S. Tourtip ,
S. Piratae ,
W. Maleewong Open the ORCID record for W. Maleewong [Opens in a new window]  and
T. Thanchomnang Open the ORCID record for T. Thanchomnang [Opens in a new window]
O. Sanpool
Affiliation:
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen40002, Thailand Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen40002, Thailand
P.M. Intapan
Affiliation:
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen40002, Thailand Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen40002, Thailand
R. Rodpai
Affiliation:
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen40002, Thailand Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen40002, Thailand
P. Laoraksawong
Affiliation:
School of Health Science, Sukhothai Thammathirat Open University Pakkret, Nonthaburi11120, Thailand
L. Sadaow
Affiliation:
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen40002, Thailand Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen40002, Thailand
S. Tourtip
Affiliation:
Faculty of Medicine, Mahasarakham University, Maha Sarakham44000, Thailand
S. Piratae
Affiliation:
One Health Research Unit, Faculty of Veterinary Sciences, Mahasarakham University, Maha Sarakham44000, Thailand
W. Maleewong
Affiliation:
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen40002, Thailand Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen40002, Thailand
T. Thanchomnang*
Affiliation:
Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen40002, Thailand Faculty of Medicine, Mahasarakham University, Maha Sarakham44000, Thailand
*
Author for correspondence: T. Thanchomnang, E-mail: thanchomnang@yahoo.com
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Abstract

Human strongyloidiasis is a deleterious gastrointestinal disease mainly caused by Strongyloides stercoralis infection. We aimed to study the possible transmission of S. stercoralis between humans and pet animals. We isolated Strongyloides from humans and domestic dogs in the same rural community in north-east Thailand and compared the nucleotide sequences of derived worms using portions of the mitochondrial cytochrome c oxidase subunit 1 (cox1) and 18S ribosomal RNA (18S rRNA) genes. Twenty-eight sequences from the 18S rRNA gene were obtained from worms derived from humans (n = 23) and dogs (n = 5), and were identical with S. stercoralis sequences (from Thailand, Cambodia, Lao PDR and Myanmar) published in the GenBank database. The 28 cox1 sequences from humans and dogs showed high similarity to each other. The available published cox1 sequences (n = 150), in combination with our 28 sequences, represented 68 haplotypes distributed among four clusters. The 28 samples from the present study represented eight haplotypes including four new haplotypes. Dogs and humans shared the same haplotypes, suggesting the possibility of zoonotic transmission from pet dogs to humans. This is of concern since dogs and humans live in close association with each other.

Keywords

Strongyloides stercoralismolecular identificationhumandogcatzoonotic transmission
Type
Research Paper
Information
Journal of Helminthology , Volume 94 , 2020 , e110
Copyright
Copyright © Cambridge University Press 2019

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