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Co-occurrence of swine cysticercosis due to Taenia solium and Taenia hydatigena in ethnic minority villages at the Thai–Myanmar border

  • K. Chaisiri (a1), T. Kusolsuk (a1), N. Homsuwan (a1), S. Sanguankiat (a1), P. Dekumyoy (a1), G. Peunpipoom (a2), S. Khiriphattharaphon (a3), Y. Sako (a4), T. Yanagida (a5), M. Okamoto (a6) and A. Ito (a4)...


As part of the international joint projects working towards the control of taeniosis/cysticercosis in Asia Pacific, epidemiological studies on Taenia solium cysticercosis have been carried out in high-incidence populations, such as minority groups in Thailand. To assess the epidemiology of cysticercotic infections in pigs in the hill-tribe minority villages (Karen) in Tak province, Thailand, we conducted serological screening and necropsies. The patterns of antibody response to T. solium antigens were then investigated using immunoblot assays. Of the 188 pig serum samples tested for antibody responses to partially purified low-molecular-weight antigens of T. solium cyst fluid, positive responses were detected in 37 samples (19.7%). Based on these results, 16 pigs (10 seropositive and 6 seronegative) were necropsied for investigation of cysticerci and intestinal parasites. All seropositive pigs were coinfected with both T. solium and Taenia hydatigena cysticerci, except one, which was infected with T. hydatigena alone. Three of the six seronegative pigs were confirmed to be infected with T. hydatigena. Pigs infected with T. solium showed much stronger antibody responses than those infected with T. hydatigena. Our results demonstrate the co-occurrence of two swine cysticercoses due to T. solium and T. hydatigena in the studied areas. This study also reveals the importance of direct confirmation of the presence of cysticerci by necropsy after serological screening. In addition to the prevalence of swine cysticercosis in these endemic areas, our findings also reveal potential implications for the development of serological diagnostic assays for swine cysticercosis.


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Author for correspondence: K. Chaisiri, E-mail: A. Ito, E-mail:


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Abuseir, S et al. (2013) An immunoblot for detection of Taenia saginata cysticercosis. Parasitology Research 112, 20692073.
Anantaphruti, MT et al. (2007) Sympatric occurrence of Taenia solium, T. saginata, and T. asiatica, Thailand. Emerging Infectious Diseases 13, 14131416.
Anantaphruti, MT et al. (2010) Molecular and serological survey on taeniasis and cysticercosis in Kanchanaburi Province, Thailand. Parasitology International 59, 326330.
Braae, UC et al. (2015) Taenia hydatigena cysticercosis in slaughtered pigs, goats, and sheep in Tanzania. Tropical Animal Health and Production 47, 15231530.
Chaisiri, K et al. (2017) Gastrointestinal helminths and Taenia spp. in parenteral tissues of free-roaming pigs (Sus scrofa indicus) from hill-tribe village at the western border of Thailand. Tropical Biomedicine 34, 464470.
Conlan, JV et al. (2012) A cross-sectional study of Taenia solium in a multiple taeniid-endemic region reveals competition may be protective. American Journal of Tropical Medicine and Hygiene 87, 281291.
Davaasuren, A et al. (2017) Neurocysticercosis: a case study of a Mongolian traveler who visited China and India with an updated review in Asia. Travel Medicine and Infectious Disease 20, 3136.
Deckers, N and Dorny, P (2010) Immunodiagnosis of Taenia solium taeniosis/cysticercosis. Trends in Parasitology 26, 137144.
Dermauw, V et al. (2016) Taenia hydatigena in pigs in Burkina Faso: a cross-sectional abattoir study. Veterinary Parasitology 230, 913.
Dorny, P et al. (2004) A Bayesian approach for estimating values for prevalence and diagnostic test characteristics of porcine cysticercosis. International Journal for Parasitology 34, 569576.
Garcia, HH et al. (2016) Elimination of Taenia solium transmission in northern Peru. New England Journal of Medicine 374, 23352344.
Ishida, MM et al. (2003) Helminth antigens (Taenia solium, Taenia crassiceps, Toxocara canis, Schistosoma mansoni and Echinococcus granulosus) and cross-reactivities in human infections and immunized animals. Acta Tropica 89, 7384.
Ito, A (2013) Nothing is perfect! Trouble-shooting in immunological and molecular studies of cestode infections. Parasitology 140, 15511565.
Ito, A and Budke, CM (2014) Culinary delights and travel? A review of zoonotic cestodiases and metacestodiases. Travel Medicine and Infectious Disease 12, 582591.
Ito, A and Craig, PS (2003) Immunodiagnostic and molecular approaches for the detection of taeniid cestode infections. Trends in Parasitology 19, 377381.
Ito, A, Takami, T and Itoh, M (1991) Vaccine effect of intact metacestodes of Taenia crassiceps against T. taeniaeformis infection in rats. American Journal of Tropical Medicine and Hygiene 44, 696701.
Ito, A et al. (1998) Novel antigens for neurocysticercosis: simple method for preparation and evaluation for serodiagnosis. American Journal of Tropical Medicine and Hygiene 59, 291294.
Ito, A et al. (1999) ELISA and immunoblot using purified glycoproteins for serodiagnosis of cysticercosis in pigs naturally infected with Taenia solium. Journal of Helminthology 73, 183186.
Ito, A et al. (2002) Dogs as alternative intermediate hosts of Taenia solium in Papua (Irian Jaya), Indonesia confirmed by highly specific ELISA and immunoblot using native and recombinant antigens and mitochondrial DNA analysis. Journal of Helminthology 76, 311314.
Ito, A, Nakao, M and Wandra, T (2003) Human taeniasis and cysticercosis in Asia. Lancet 362, 19181920.
Ito, A et al. (2014) The present situation of human taeniases and cysticercosis in Asia. Recent Patents on Anti-Infective Drug Discovery 9, 173185.
Ito, A, Yanagida, T and Nakao, M (2016) Recent advances and perspectives in molecular epidemiology of Taenia solium cysticercosis. Infection, Genetics and Evolution 40, 357367.
Kobayashi, K et al. (2013) Rare case of disseminated cysticercosis and taeniasis in a Japanese traveller after returning from India. American Journal of Tropical Medicine and Hygiene 89, 5862.
Krecek, RC et al. (2008) Prevalence of Taenia solium cysticercosis in swine from a community-based study in 21 villages of the Eastern Cape Province, South Africa. Veterinary Parasitology 154, 3847.
Kungu, JM et al. (2017) Sero-prevalence of Taenia spp. cysticercosis in rural and urban smallholder pig production settings in Uganda. Acta Tropica 165, 110115.
Li, T et al. (2006) Taeniasis/cysticercosis in a Tibetan population in Sichuan Province, China. Acta Tropica 100, 223231.
Li, T et al. (2007) Taeniasis/cysticercosis in China. Southeast Asian Journal of Tropical Medicine and Public Health 38 (Suppl 1), 131139.
Lightowlers, MW (2010) Fact or hypothesis: concomitant immunity in taeniid cestode infections. Parasite Immunology 32, 582589.
Lightowlers, MW (2013) Control of Taenia solium taeniasis/cysticercosis: past practices and new possibilities. Parasitology 140, 15661577.
Lightowlers, MW et al. (2015) Sensitivity of partial carcass dissection for assessment of porcine cysticercosis at necropsy. International Journal for Parasitology 45, 815818.
Lightowlers, MW et al. (2016) Monitoring the outcomes of interventions against Taenia solium: options and suggestions. Parasite Immunology 38, 158169.
McCleery, EJ et al. (2015) Taeniasis among refugees living on Thailand–Myanmar border. Emerging Infectious Diseases 21, 18241826.
Muro, C et al. (2017) Porcine cysticercosis: possible cross-reactivity of Taenia hydatigena to GP50 antigen in the enzyme-linked immunoelectrotransfer blot assay. American Journal of Tropical Medicine and Hygiene 97, 18301832.
Nguyen, MT et al. (2016) A systematic review on the global occurrence of Taenia hydatigena in pigs and cattle. Veterinary Parasitology 226, 97103.
Pawlowski, Z (2016) Taeniosis/neurocysticercosis control as a medical problem—a discussion paper. World Journal of Neuroscience 6, 165170.
Phiri, IK et al. (2002) The prevalence of porcine cysticercosis in eastern and southern provinces of Zambia. Veterinary Parasitology 108, 3139.
R Core Team (2015) R: A Language and Environment for Statistical Computing. Available from: [accessed 19 November 2017].
Rózsa, L, Reiczigel, J and Majoros, G (2000) Quantifying parasites in samples of hosts. Journal of Parasitology 86, 228232.
Sako, Y et al. (2013) Simple and reliable preparation of immunodiagnostic antigens for Taenia solium cysticercosis. Parasitology 140, 15891594.
Sato, MO et al. (2003) Evaluation of tongue inspection and serology for diagnosis of Taenia solium cysticercosis in swine: usefulness of ELISA using purified glycoproteins and recombinant antigen. Veterinary Parasitology 111, 309322.
Sato, MO et al. (2018) Taenia solium, Taenia saginata, Taenia asiatica its hybrids and other helminthic infections occurring in a Neglected Tropical Diseases’ highly endemic area in Lao PDR. PLoS Neglected Tropical Diseases 12, e0006260.
Schantz, PM, Wilkins, PP and Tsang, VCW (1998) Immigrants, imaging, and immunoblots: the emergence of neurocysticercosis as a significant public health problem. Emerging Infections 2, 213242.
Sciutto, E et al. (1998) Limitation of current diagnostic procedures for the diagnosis of Taenia solium cysticercosis in rural pigs. Veterinary Parasitology 79, 299313.
Sikasunge, CS et al. (2008) Prevalence of Taenia solium porcine cysticercosis in the eastern, southern and western provinces of Zambia. Veterinary Journal 176, 240244.
Subahar, R et al. (2001) Taenia solium infection in Irian Jaya (West Papua), Indonesia: a pilot serological survey of human and porcine cysticercosis in Jayawijaya District. Transactions of the Royal Society of Tropical Medicine and Hygiene 95, 388390.
Swastika, K et al. (2016) Swine cysticercosis in the Karangasem district of Bali, Indonesia: an evaluation of serological screening methods. Acta Tropica 163, 4653.
Wandra, T et al. (2016) Neurocysticercosis diagnosed in a patient with Taenia saginata taeniasis after administration of praziquantel: a case study and review of the literature. Primary Healthcare: Open Access 6, 14.
WHO (World Health Organization) (2016) Landscape Analysis: Management of Neurocysticercosis with an Emphasis on Low- and Middle-income Countries. Geneva: World Health Organization.
Wu, HW et al. (2017) Cysticercosis/taeniasis endemicity in Southeast Asia: current status and control measures. Acta Tropica 165, 121132.
Yang, HJ et al. (1998) Immunoblot analysis of a 10 kDa antigen in cyst fluid of Taenia solium metacestodes. Parasite Immunology 20, 483488.



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